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Biomedical subjects

S Guibaud

Publications and source records attributed to S Guibaud.

At least 55 records · Page 3Linked to original sources
PubMed1979

Alpha-fetoprotein levels in placenta, maternal, and cord blood in normal and pathologic pregnancy.

The level of placental alpha-fetoprotein (AFP) in normal pregnancy at term was found to be 5050 +/- ng/g fresh tissue. A significant increase was noted in the toxemic placenta associated with a rise in circulating maternal AFP level and a nonsignificant increase in cord blood. The increase in maternal AFP level in cases of premature delivery before 33 weeks' gestation and postmature delivery after 40 weeks' gestation predicted that maternal AFP measurement throughout the second and third trimester of pregnancy could be clinically established as an index of placental function.

Female↗
PubMed1979

[Significance and limits of alpha-fetoprotein levels in the prenatal diagnosis of malformations of the central nervous system].

Amniotic fluid or/and serum alpha-foeto protein (AFP) determination is used as a test-system for screening of neural tube defects. The physiologic basis of this screening are described taking into account the evolution of AFP concentration in foetal and maternal blood, and in amniotic fluid. As for all the biologic screening systems, the acquired experience reveals a lack of sensibility and specificity. First the authors analyse the analytic and biologic problems which have an effect upon sensibility; then, they consider the mechanisms which explain the specificity lack showing itself in some foetal malformations. Practically, interpretation of AFP results requires necessarily familial story and echography results. Taking into consideration the different problems concerning AFP, a programme to utilize the test-system is presented for prenatal diagnosis of malformations of central nervous system.

Amniotic Fluid↗
PubMed1978

Purification of placental alpha-1-foetoprotein.

Quantitation of placental alpha-1-foetoprotein was done by the radioimmunoassay technique and gave a mean value of 6060 +/- 22.2 ng/g fresh tissue. The purification process included three methods. (1) Protein precipitation was performed using ammonium sulphate at 50 and 70% saturation. Elution on a Concanavalin A-sepharose column was used to diminish the interference of albumin with alpha-foetoprotein. (2) A coupling immunoadsorption technique using CNBr-activated Sepharose 4-B, antialbumin and antitransferrin, was found to be more reproducible. (3) Counter-immunoelectrophoresis and discontinuous gel electrophoresis gave a 60% yield with a 400-fold purification.

Chromatography, Affinity↗
PubMed1978

[Copper intra-uterine devices (I.U.D.) and pregnancy: a study of the metabolism of copper and of ceruloplasmin in the mother and in the products of conception (author's transl)].

The authors studied the variations in levels of copper and ceruloplasmin in the mother and in products of conception during the first trimester of pregnancy, during the 4th month of pregnancy and near term, having a series of 12 pregnancies with the copper I.U.D. in position. No change was observed between the levels of copper and ceruloplasmin in comparison with a control series.

Abortion, Induced↗
PubMed1977

[Ethanol perfusions during threatened premature labor. Special study of oxytocinase activity].

The anti-oxytocic effect of perfusions of ethanol have been studied in 19 cases of threatened premature labour. Ethanol has a muscle-relaxing action on the uterus which was demonstrated in 8 out of the 19 cases. Blood alcohol levels equal at least to 1 gm per litre seem to be necessary to have any chance of success and furthermore action has to be rapid and given in loading doses. There are secondary effects from ethanol perfusions which, the authors consider, considerably limit the use of this product in threatened premature labour. Furthermore, when levels of the ocytocinase effect on the serum was studied in 10 cases before and after transfusing the ethanol, the results showed that there was little likelihood that overactivity of ocytocinase could explain the lowering in the levels of oxytocins brought about by the use of ethanol.

Aminopeptidases↗
PubMed1975

[Alpha fetoprotein in umbilical cord blood].

Quantitative measurement of alpha-foetoprotein was made on the cord blood of 158 new-born of gestational age ranging between 15 and 43 weeks. The technique used was that of simple radial immunodiffusion of Mancini which made possible the exact measurement of A.F.P. in all the specimens apart from 3. The correlation coefficient between the A.F.P. level in cord blood and gestational age is significant (r equals 0,85 for a risk p equals 0,001). However, the degree of difference at a given point in pregnancy and the difference in levels found in certain cases of twin pregnancies, suggest that factors other than gestational age (certain complications of pregnancy, for example) may influence foetal A,F.P.

Female↗
PubMed1975

Alpha 1-antitrypsin in amniotic fluid.

Amniotic fluid a1-antitrypsin values were determined by radial immunodiffusion of 317 samples obtained between the 10th and 43rd weeks of gestation in normal pregnant patients and those with complications. Serial determinations of a1-antitrypsin values revealed that in normal patients values decreased as pregnancy advanced while values increased as pregnancy advanced in cases in which there was severe Rh-alloimmunization and hydramnios. A wide range of values was observed for each week of gestation. Although there was a definite tendency for values to decrease as pregnancy advanced (60.8 mg/100 ml before 30 weeks and 17.9/100 ml after 40 weeks); the method must be regarded as unreliable for determining fetal maturity in clinical practice. In the case of newborn infants with hyaline membrane disease, no decrease in amniotic fluid a1-antitrypsin values has been found.

Amniotic Fluid↗