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S Hauschka

Publications and source records attributed to S Hauschka.

9 recordsLinked to original sources
PubMed1991

The MCK enhancer contains a p53 responsive element.

p53 is an antioncogene that is defective or absent in a large number of human tumors. Its function in normal cells is not known. We show that co-transfection of mouse p53 with muscle-specific creatine kinase-chloramphenicol acetyltransferase reporter gene, containing 3.3 kilobase of upstream control sequence for the muscle-specific creatine kinase gene, results in a 10- to 80-fold activation. The p53 responsive element maps to a region distinguished from the known MyoD binding region. Identification of a p53 responsive element should allow a more focused analysis of the effects of p53 in controlling gene activity.

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PubMed1983

Spatial analysis of limb bud myogenesis: elaboration of the proximodistal gradient of myoblasts requires the continuing presence of apical ectodermal ridge.

Myogenic tissue from embryonic chick wing and leg buds is composed of several subpopulations of myoblasts. These clonally distinct subpopulations first appear at different developmental stages, and are distributed differently along the proximo-distal axis of the buds, giving the appearance of a gradient of myoblast cell types. This myoblast distribution pattern has been utilized to investigate the dependence of muscle tissue outgrowth and development on the presence of the apical ectodermal ridge (AER). Wing buds which have had the AER removed at stages 17-18 (2 days) subsequently develop normal proximal regions, but fail to elaborate skeletal structures distal to the humerus. The myoblast pattern of operated buds is also normal proximally, but distal portions of the pattern are not observed. Removal of the AER at stage 20 (3 days) results in buds which develop slightly more distal skeletal structures and the coinciding portions of the myoblast pattern, but in which the more distal portions of the normal myoblast gradient are truncated. These data suggest that elaboration of the myogenic pattern in early limb buds is dependent on the continuing presence of the AER, and that early removal of the AER leads to the subsequent cessation of myoblast pattern specification.

Animals↗