The MCK enhancer contains a p53 responsive element.

H Weintraub; S Hauschka; S J Tapscott

doi:10.1073/pnas.88.11.4570

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The MCK enhancer contains a p53 responsive element.

H Weintraub·S Hauschka·S J Tapscott

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Abstract

p53 is an antioncogene that is defective or absent in a large number of human tumors. Its function in normal cells is not known. We show that co-transfection of mouse p53 with muscle-specific creatine kinase-chloramphenicol acetyltransferase reporter gene, containing 3.3 kilobase of upstream control sequence for the muscle-specific creatine kinase gene, results in a 10- to 80-fold activation. The p53 responsive element maps to a region distinguished from the known MyoD binding region. Identification of a p53 responsive element should allow a more focused analysis of the effects of p53 in controlling gene activity.

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Animals Cell Line Chloramphenicol O-Acetyltransferase Creatine Kinase DNA-Binding Proteins Enhancer Elements, Genetic Genetic Vectors Humans Isoenzymes Mice Muscle Proteins Muscles MyoD Protein Plasmids Tumor Suppressor Protein p53 Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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H Weintraub, S Hauschka, S J Tapscott. 1991-06-01. The MCK enhancer contains a p53 responsive element.. https://doi.org/10.1073/pnas.88.11.4570

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