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Biomedical subjects

S I Danev

Publications and source records attributed to S I Danev.

3 recordsLinked to original sources

[A method of determining glucose oxidase-immobilized glucose].

A method for manual measurement of glucose in biologic fluids has been developed, making use of glucose oxidase immobilized on a carbamide derivative of microcrystal cellulose; two variants are suggested: a rapid and a routine one. The method is characterized by a high analytical reliability, its results are in high correlation with the results of measurements by Beckman glucose analyzer (r = 0.92, p less than 0.001). The method is economic (glucose oxidase reagent may be used for more than 300 times), easily available, and is 3 to 6 times more rapid than the method with soluble glucose oxidase. It is particularly convenient for urgent laboratory diagnosis.

Blood Glucose↗

[A method for determining urea by using immobilized urease].

An available method was developed for manual measurements of urea in biological fluids with the use of urease immobilized in sepharose gel. The suggested technique is more reagent-saving (the same reagent may be used for up to 500 times), 3-6-fold more rapid, and twice more sensitive than the routine phenolhypochlorite method with soluble urease. The method is characterized by a high analytical reliability, its results are in high correlation with the soluble urease procedure and with the diacetylmonoxime method (r = 07988 and 0.995, respectively). It is particularly fit for rapid analysis of the urea and convenient for small laboratories.

Enzymes, Immobilized↗