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PubMed · 1691334

[A method for determining urea by using immobilized urease].

I P Ivanov·S I Danev·Ch D Palashev·D G Dimitrov
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Abstract

An available method was developed for manual measurements of urea in biological fluids with the use of urease immobilized in sepharose gel. The suggested technique is more reagent-saving (the same reagent may be used for up to 500 times), 3-6-fold more rapid, and twice more sensitive than the routine phenolhypochlorite method with soluble urease. The method is characterized by a high analytical reliability, its results are in high correlation with the soluble urease procedure and with the diacetylmonoxime method (r = 07988 and 0.995, respectively). It is particularly fit for rapid analysis of the urea and convenient for small laboratories.

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Enzymes, ImmobilizedHumansHydrolysisIndicators and ReagentsKidney DiseasesPhenolPhenolsReproducibility of ResultsSepharoseUreaUreaseComparative StudyEnglish AbstractJournal Article

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I P Ivanov, S I Danev, Ch D Palashev, D G Dimitrov. 1990. [A method for determining urea by using immobilized urease].. https://pubmed.ncbi.nlm.nih.gov/1691334/

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