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Biomedical subjects

S I Kuznetsov

Publications and source records attributed to S I Kuznetsov.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[Treatment of acute experimental diphtheria toxemia by immunohemosorption].

The antitoxic antidiphtheric immunosorbent DATs was tested in experiments on mice and guinea-pigs using the model of acute diphtheric toxemia. In extracorporeal connecting with the circulation system of the animals the sorbent effectively bound and eliminated from the circulation molecules of diphtheric toxin and antitoxin, reduced significantly their levels in the blood after hemoperfusion. The effectiveness of the immunosorbent DATs was confirmed when it saved the ginea-pigs after injection of lethal toxin doses.

Acute Disease↗
PubMedDate not supplied

[Hemosorption in serotherapy (serosorption) in experimental acute diphtheric toxemia with the use of affinity sorbents].

In modeling acute diphtheric toxemia in guinea pigs, the authors propose to perform hemosorption using selective sorbents under continuous introduction of antitoxic immune sera into extracorporeal contour in front of sorption column. Combination of hemosorption with serotherapy in the same time interval may be denoted as serosorption. As specific sorbents, affine preparations imasorb A-700 and Imasorb G-700 were used. They selectively eliminate from the blood flow CIC produced by diphtheric toxin (anatoxin) and antitoxic rabbit and horse antibodies. Changes in the titers of the diphtheric toxin (anatoxin) and CIC in blood evidence for efficiency of selective sorbents were confirmed by immunohistochemical analysis of the preparations' granules after hemoperfusion.

Animals↗
PubMedDate not supplied

[Determination of the glucose reserves in the water of the Rybinsk reservoir].

The concentrations of glucose (Km + Sn) was determined by the method of Parson and Strickland in the water of the Rybinsk and Sheksna Reservoirs. It varied from 11 to 31 mcg C per litre and reached 380 mcg C per litre in swamp waters. The rate of glucose assimilation by the microflora varied from 0.02 to 0.56 mcg C per litre per hour and reached 5--7 mcg C per litre per hour in contaminated waters.

Glucose↗
PubMedDate not supplied

[Assessment of total complement activity of blood plasma (serum) using reader for immunoenzyme analysis].

A method for instrumental assessment of the total complementary activity of human blood plasma (serum) by the classical and alternative activation is proposed. The test is carried out by the micromethod in round-bottom 96-well plates for immunological tests. The results are read by automated analyzer for enzyme immunoassay recording at 405 nm wavelength.

Complement Activation↗
PubMedDate not supplied

[Determination of circulating immune complexes by immunoenzyme method].

Enzyme immunoassay is proposed to be used for measuring the content of circulating immune complexes (CIC) in human blood serum. S. aureus protein in a concentration of 1 to 5 micrograms/ml optimal for the sensitization of plates is used as the first sandwich layer. The method permits registration of CICs formed by immunoglobulins of different classes. Investigations of the blood sera of donors and cancer patients by the proposed method and by other methods (V. Haskova et al. and M. Digeon et al.) to assess complex formation showed a good correlation of the results of all measurements.

Antigen-Antibody Complex↗
PubMedDate not supplied

[Immune system cells as mediators in the reaction of other body systems to stress exposure].

This paper first details an acute reaction of mice to the administered syngenic and allogenic live splenocytes from the animals exposed to great emergency. The authors have called it "nonspecific immunogenic shock" as its stages and manifestations which are characteristic of shock are caused only by lymphocytes, but associated with any specific exposure. The most common sequela of nonspecific immunogenic shock may be animal's death within minutes or first days after administration of splenocytes. In this case, marked cataracts (generally after death, but occasionally in life) appear both in recipients and stress-exposed donors. The ability of splenocytes activated by stress in donors to cause sudden or delayed death of most recipients makes the author give the second term of "death transfer" to nonspecific immunogenic shock.

Animals↗
PubMedDate not supplied

[Means for the rational utilization of the bed resources under obligatory medical insurance].

Presents a multivariant system of rational use of the bed fund used in Samara Province [correction of district]. This system helped preserve the scope of hospital aid rendered to the population in 1993-1995 which was planned by the district program of obligatory medical insurance, despite cutting down about 5,000 beds in premises previously adjusted for therapeutic needs and old premises.

Bed Occupancy↗
PubMedDate not supplied

[Classification approach to prognostication of the severity of bronchial asthma].

To define the severity of bronchial asthma, to predict its subsequent course, and to prescribe adequate glucocorticosteroid therapy, a mathematical model based on the cluster analysis was used. The authors' algorithm of the classification of bronchial asthma was based on the cluster analysis was used. The authors' algorithm of the classification of bronchial asthma was based on the mean distance value between the objects characterizing patients' status and hyperspatial signs. Each patient was described using a set of 15 clinical, functional, and immunological indices which had a meaning of signs describing the objects. Three clusters corresponding to the groups identified a priori up to two patients were formed to make the classification by the algorithm proposed. The findings make it possible to efficiently analyze the baseline indices in a particular patient from his entrance into or highest relationship to a definite cluster. This may define the severity of bronchial obstruction and predict its progression.

Algorithms↗