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S Ino

Publications and source records attributed to S Ino.

34 records · Page 2Linked to original sources
PubMed1983

A sensitive assay of sialidase activity with fluorogenic oligosaccharide derivatives.

Fluorogenic oligosaccharide derivatives, lactityl-aminopyridine and sialyllactityl-aminopyridines, were synthesized by reductive amination of lactose and sialyllactose with 2-aminopyridine in the presence of sodium cyanoborohydride. Lactityl-aminopyridine showed bluish-white fluorescence at 390 nm by excitation with ultraviolet light at 320 nm, and the intensity of the fluorescence was proportional to the concentration of lactityl-aminopyridine within a range from 20 to 9800 pmole/ml. Two isomers of sialyllactityl-aminopyridine were enzymatically hydrolyzed to sialic acid and lactityl-aminopyridine by the action of both microbial and mammalian sialidases, and their suitability as substrates for sialidase was investigated. The result showed that sialidase activity was determined with about 55-fold higher sensitivity than that of ordinarily used colorimetric methods.

Aminopyridines↗
PubMed1981

Abnormal urinary excretion in sialoglycoconjugates in patients with mucopolysaccharidosis.

Abnormal urinary excretion of sialoglycoconjugates was observed in four patients with mucopolysaccharidosis. Urinary sialoglycoconjugates were fractionated into 8 fractions by sequential gel filtration on Sephadex G-25, G-50 and by Dowex 1 ion-exchange chromatography. The comparison of the amounts of these fractions indicated that the fraction rich in mannose and glucosamine contents contributed to the increased urinary excretion of sialoglycoconjugates in patients with mucopolysaccharidosis. The major component of this fraction was disialyl-oroso-N-octaose which was a representative oligosaccharide side chain of glycoproteins with an Asn-GlcNAc linkage. Undoubtedly this abnormality is the secondary lesion of mucopolysaccharidosis, but it is conceivable that the disturbed metabolism of sialoglycoprotein is closely related to the pathogenesis of these diseases.

Adolescent↗