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S Inutsuka

Publications and source records attributed to S Inutsuka.

At least 37 records · Page 2Linked to original sources
PubMed1993

Inhibition of PHA induced mononuclear cell proliferation by FK506 in combination with cyclosporine, methylprednisolone, 6-mercaptopurine and mycophenolic acid.

In vitro inhibition of human peripheral blood mononuclear cell (PBMC) proliferation by immunosuppressive drugs has been shown to correlate with clinical outcomes in kidney transplant patients. The aim of our study was to analyse the degree of variability of in vitro interactions of FK506 (FK) with combinations of cyclosporin A (CyA), methylprednisolone (MP), 6-mercaptopurine (6ME), and mycophenolic acid (MPA) using phytohaemagglutinin (PHA) stimulated PBMCs. Our hypotheses were: that a wide range of interindividual variation would be detected; and that certain combinations of drugs would have a synergistic inhibitory effect on PHA stimulated PBMCs. Cells were cultured in supplemented RPMI 1640 for 65 hours at 37 degrees C in humidified 5% CO2 - 95% air and proliferative responses measured by 3H-thymidine incorporation over a further 24 hours. Inhibition of PBMC proliferation was assessed over 10 FK concentrations ranging from 1 x 10(-8) to 10 micrograms/ml using both volunteer controls (n = 51) and dialysis patients (n = 23). A wide range of interindividual variability of FK inhibition occurred throughout the range of FK concentrations tested, becoming less variable at the higher concentrations. The interactions of FK with CyA, MP, 6ME and MPA were assessed using PBMCs from 12 volunteer controls. For FK at 1 x 10(-8) micrograms/ml; CyA, MP, 6ME and MPA were used at 0.01 microgram/ml. For FK at 1 x 10(-7) micrograms/ml; CyA, MP, 6ME and MPA were used at 0.1 microgram/ml.(ABSTRACT TRUNCATED AT 250 WORDS)

Cyclosporine↗
PubMed1993

[Potentiation of cytotoxicity of anticancer drugs by flavone acetic acid].

We used B 16 melanoma cells both in vitro and in vivo to determine whether Flavone acetic acid (FAA) can increase the cytotoxicity of mitomycin C (MMC) under normothermic and hyperthermic (HT) conditions. The significantly increased cytotoxicity of MMC combined with FAA and HT seems to be mainly related to activation of alkylating species under FAA mediated hypoxic condition.

Animals↗
PubMed1993

[Experimental and clinical study in vitro chemosensitivity test for succinate dehydrogenase inhibition test].

The usefulness and problems in the succinate dehydrogenase inhibition (SDI) test as an in vivo chemosensitivity test were reviewed and discussed. SDI test is based on the correlation of succinate dehydrogenase activity with cell viability. The optimal condition of SDI test were evaluated with respect to concentration of anticancer drugs, incubation time, effect of sodium succinate on formazan production and enzymatic disaggregation of solid tumors. Analysis of the chemosensitivity of clinical tumors revealed that the origin of tumor, histological differentiation, DNA distribution patterns, estrogen receptor status in breast cancer, and difference of primary and metastatic lesions were critical for determining the chemosensitivity of tumor tissues. Correlation between in vitro sensitivity in the SDI test and in vivo clinical results of chemotherapy was evaluated. Since SDI test reflects cell viability and is a simple, rapid and inexpensive methods, it is appropriate both for clinical chemosensitivity testing and for the screening of anticancer drugs.

Colonic Neoplasms↗
PubMed1993

[Fluorocytometric DNA analysis of intestinal metaplasia associated with gastric carcinoma].

Histopathologic studies and fluorocytometric DNA analysis were carried out in 39 patients with intestinal metaplasia (IM) of the mucosa associated with gastric carcinoma, in order to determine possible relationships. According to the histological degree of IM, 39 cases were divided into 16 (41%) with slight-to-moderate, and 23 (59%) with severe IM. The 39 tumors were histologically divided into 20 (51%) differentiated tumors and 19 (49%) undifferentiated tumors. The DNA patterns of tumors revealed 17 (43%) to be euploidy and 22 (57%) to be aneuploidy. The DNA content of the metaplastic epithelial cells was measured, and the percentage of the cells in S and G2 + M phases of the cell cycle was taken as the index of proliferative activity. DNA analysis of the non-metaplastic epithelium was performed in 10 as a control group. While the histological differentiation of the tumors and the degree of IM were not related to the proliferative activity of IM, the IM around the aneuploid tumors did have higher proliferative activity (14.01 +/- 6.16%) than that around euploid tumors (11.08 +/- 7.13%) or of normal mucosa (9.95 +/- 9.92%). This approach will help to determine cell kinetics of IM around the gastric tumor. Our study indicated a relationship between DNA ploidy and the proliferative activity of IM around gastric cancer.

Cell Cycle↗
PubMed1993

[Clinical significance of flow cytometric DNA analysis in patients with rectal cancer].

DNA content was measured by flow cytometry using paraffin-embedded material from 148 primary rectal cancers and 10 distant metastatic lesions. DNA ploidy pattern was classified into the following three groups in terms of the DNA index: 1.00 was defined as the diploid group (DP), 1.00 < DNA index < 1.60 as the low-aneuploid group (LAP) and > or = 1.60 as the high-aneuploid group (HAP). Primary carcinomas were DP in 26.4%, LAP in 36.5% and HAP in 37.1%. The DNA ploidy pattern of the primary tumor correlated well with clinicopathological findings such as depth of invasion, lymphatic invasion, pathological stage, metastasis to distant organs and curability of the tumor. In patients with HAP tumor after curative operation, the recurrence rate (21.6%) in distant organs was significantly higher than those with DP tumor (2.8%) and LAP tumor (6.4%) [p < 0.05]. Two LAP patients and 8 HAP patients with distant metastatic disease had the same DNA ploidy pattern HAP in the metastatic lesions. These data indicate that tumor DNA ploidy patterns classified into three groups in rectal cancer may play an important role in predicting prognosis, including distant metastasis.

DNA, Neoplasm↗
PubMed1992

Radical lymphadenectomy for rectal cancer facilitated by a carbon particle infusion lymphangiography.

For the intraoperative visualization of the para-aortic nodes and those around the iliac vessels, a fine carbon particle solution was infused into the bilateral pedal lymphatic vessels of 12 patients with rectal carcinoma. A low anterior resection with radical lymph node dissection was then performed while preserving pelvic autonomic nerves. Of 444 lymph nodes removed from the iliac arterial region, 430 were stained with carbon black (96.8%), even though the black staining was not perfect in the nodes of the inferior mesenteric arterial region. All of the lateral black stained nodes were clearly visible and hence could be easily excised. The average number of dissected nodes in one patient was 43.8 in this dissection with carbon particle infusion, which was larger than those of conventional lymph node dissection. We then examined the length of time that a postoperative indwelling bladder catheter was needed as an indication for autonomic nerve damage, and it was ascertained that less damage occurred in this operation compared to other types of dissections, such as conventional or extended lymph node dissection.

Carbon↗
PubMed1992

Diaphragmatic cyst with elevated level of serum tissue polypeptide antigen.

A 58-year-old woman with a mesothelial cyst of the diaphragm showed high level of serum Tissue Polypeptide Antigen (TPA) and cyst fluid TPA. Mesothelial cells of the cyst may excrete TPA and TPA was accumulated in the cysts. The measurement of tumor marker such as TPA of the serum or of the content of the cyst is useful in observation of the clinical course.

Biomarkers, Tumor↗
PubMed1992

Herpes zoster in patients with sarcoidosis.

108 patients with sarcoidosis were retrospectively studied for the development of herpes zoster. Five of these patients (4.6%), 2 of whom were in their twenties, developed herpes zoster. Only 1 patient had been treated with an oral steroid. All 5 had extrathoracic lesions. Zoster tended to occur during the inactive stage of sarcoidosis and did not exacerbate the activity of the sarcoidosis. The clinical course of their zoster infection was typically benign. There have been few reports of herpes zoster in patients with sarcoidosis. Further studies are required to determine whether sarcoidosis predisposes to herpes zoster infection.

Adolescent↗
PubMed1992

Adenocarcinoma in Meckel's diverticulum: report of a case and review of 30 cases in the English and Japanese literature.

A 54-yr-old man who complained of abdominal pain was found to have an adenocarcinoma arising in Meckel's diverticulum, as preoperatively diagnosed with 99mTc-pertechnetate scintigraphy. Angiography of the superior mesenteric artery revealed multiple branched arteries and tumor stain, but the vitelline artery was not clearly identified. Surgery revealed that the tumor had invaded the urinary bladder and the ileum, including the diverticulum, and the bladder had to be partially resected. Histopathological examination of the lesion revealed a diverticulum containing normal small bowel mucosa, ectopic normal gastric tissue, and adenocarcinoma. In a review of 30 cases of adenocarcinoma in Meckel's diverticulum in the English and Japanese literature, our case was the first to be diagnosed preoperatively.

Adenocarcinoma↗
PubMed1991

Adverse effect of chronic tonsillitis on clinical course of sarcoidosis.

Sarcoidosis resolves spontaneously in many cases on a long-term basis. However, there are many cases whose resolution is delayed. We have experienced a frequent association of chronic tonsillitis (CTL), and dental caries in those patients of persistent course. We hypothesized that the presence of chronic or recurrent inflammatory foci in the upper airway may adversely affect the natural resolution of sarcoidosis. We analyzed the difference of regression of bilateral hilar lymphadenopathy on chest roentgenogram (CXR) in relation to changes in serum angiotensin converting enzyme (SACE) in sarcoidosis patients with and without CTL, and the effect of tonsillectomy (TLT) on the clinical course. In this study, we focused only on the tonsil for the sake of simplicity of analysis. Sarcoidosis resolved spontaneously in 10 of 28 (36%) patients with CTL and in 19 of 25 (76%) without CTL, and after TLT 9 cases became favorable in the course and the rate of unfavorable turned similar to those without CTL. The presence of CTL may be adversely related to the resolution of sarcoidosis.

Adolescent↗
PubMed1990

An adapter for stapling of a colorectal anastomosis.

A technique involving the use of an end-to-end anastomosing stapler for low anterior resection has been modified to allow for a low colorectal anastomosis with an anus-pushing rubber adapter. The facility and safety of this procedure, under direct vision, has been confirmed.

Anastomosis, Surgical↗
PubMed1990

Dilation of the stump of the esophagus and intestine with Doyen's intestinal clamp followed by application of lidocaine facilitates the stapling anastomoses.

To avoid bowel tears on the esophagojejunal or colorectal mechanical anastomoses during insertion of the EEA stapler, we applied a local anesthetic to the stump of the bowel, then gently dilated the bowel with Doyen's intestinal clamp. This approach makes way for use of a larger cartridge and a more satisfactory anastomoses.

Anastomosis, Surgical↗
PubMed1988

Characteristic radiographic features of pulmonary carcinoma associated with large bulla.

Primary lung cancer closely associated with a large bulla was investigated in an attempt to elucidate radiographic features of the tumor. On the basis of findings in 7 of our patients plus data on another 25 patients in the Japanese literature, we propose three major patterns of neoplasm development: nodular opacity within or adjacent to the bulla, partial or diffuse thickening of the bulla wall, and secondary signs of the bulla (changed diameter, fluid retention, and pneumothorax). As the incidence of occurrence of a pulmonary carcinoma in association with bullous disease is high, these radiographic findings will aid in early detection of a malignant lesion.

Adenocarcinoma↗
PubMed1988

Serum testosterone level in the peripheral vein and the perigastric draining vein of patients with gastric carcinoma.

The serum testosterone level (STL) was determined in 181 patients with gastric carcinoma, 128 male and 53 female, and in 98 patients with benign diseases, 67 male and 31 female. As in our previous report, the preoperative STL of the cancer group was significantly lower than that of the control group, in both male and female patients (P less than 0.001). In the present study, the STL of the perigastric vein draining the stomach was determined and compared with the STL of the blood obtained at the same time from the peripheral vein during the operation. It was found that, in the male patients, the STL of the blood obtained from the perigastric vein draining a stomach with carcinoma was significantly lower than that of the peripheral vein in the same patient (P less than 0.001); however, there was no difference between these STL in the control group. On the contrary, in the female patients during operation, no such phenomenon was confirmed. Analysis classifying the patients by histological type revealed almost the same results for all patients as those shown for the cancer group, i.e. there was a proven significant difference in male patients and no significant difference in female patients. It was therefore considered that the mechanism of decrease in preoperative peripheral STL was not always the same in male patients as in female patients, although the STL decrease was observed to be similar in both sexes before surgery.

Adenocarcinoma↗
PubMed1988

[A minute, solitary carcinoid of the stomach with a lymph node metastasis--a case report].

The patient, a 25-year-old woman, complained of epigastralgia. Her physical examination and routine laboratory data were unremarkable, though an upper G-I series and a gastro-endoscopy demonstrated a small sessile polypoid lesion at the posterior wall of the gastric body. An endoscopic biopsy specimen taken from the polyp revealed a typical carcinoid tumor. The patient underwent a wedge resection of the stomach and a sampling was taken of the regional lymph node around the left gastric vessels. Histologically, although the carcinoid tumor was 5 mm in diameter and limited to within the submucosal layer, it showed lymphatic and venous invasion into the submucosal layer and had metastasized to one of the regional lymph nodes around the left gastric vessels. Therefore, a total gastrectomy with an extended lymph node dissection was performed and no other metastatic lesion was uncovered. Of the reported cases with a gastric carcinoid thus far, none with a tumor size of less than 5 mm has evidenced metastasis. This case suggests that even cases of a minute gastric carcinoid should be treated by radical gastrectomy and extended lymph node dissection.

Adult↗