Oligonucleotide-directed random mutagenesis using the phosphorothioate method.
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Biomedical subjects
Publications and source records attributed to S J Dale.
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A method is described using dried reagents in microtiter plates for direct PCR and sequencing of M13 plaques. The method has advantages of reliability and high throughput that make it suitable for medium to large-scale sequencing projects.
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Ankle sprains resulting in chronic ankle instability can be very disabling for the patient. There have been several procedures developed to stabilize the lateral aspect of the ankle. It will be shown that the Lee ankle stabilization is an appropriate procedure when surgical correction is indicated for the chronic unstable ankle. An important aspect of this particular procedure is the use of a periosteal flap, from the lateral malleolus, with the peroneus brevis tendon.
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The authors report on the results of eight calcaneal fractures treated conservatively with the Robert-Jones compression dressing technique. They believe that conservative treatment of fractures of the calcaneous, the most frequently fractured tarsal bone, is successful and nonunions are rare. Also, the risk of complications is lessened and the results are uniformly satisfactory.