PubMed HealthSearch

PubMed · 8850022

Oligonucleotide-directed random mutagenesis using the phosphorothioate method.

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

S J Dale, M Belfield. 1996. Oligonucleotide-directed random mutagenesis using the phosphorothioate method.. https://doi.org/10.1385/0-89603-332-5%3A369

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Some results on combinations of two binary screening tests.

When two medical screening tests for a disease exist, it may be appealing to combine them to increase screening accuracy. Combination of two valid screening tests, where each independently has screening performance, can increase the overall disease screening performance. Some results are obtained when two binary screening tests are combined according to the Boolean Any+ strategy, as defined by Maguire (1). These results are useful and provide investigators of two screening tests a sense of what to expect of the performance of the two tests when combined. This then may be followed by a clinical trial that directly demonstrates the benefit of the combined test.

Binomial Distribution

Improved confidence intervals for the difference between binomial proportions based on paired data.

Existing methods for setting confidence intervals for the difference theta between binomial proportions based on paired data perform inadequately. The asymptotic method can produce limits outside the range of validity. The 'exact' conditional method can yield an interval which is effectively only one-sided. Both these methods also have poor coverage properties. Better methods are described, based on the profile likelihood obtained by conditionally maximizing the proportion of discordant pairs. A refinement (methods 5 and 6) which aligns 1-alpha with an aggregate of tail areas produces appropriate coverage properties. A computationally simpler method based on the score interval for the single proportion also performs well (method 10).

Binomial Distribution

Interval estimation for the difference between independent proportions: comparison of eleven methods.

Several existing unconditional methods for setting confidence intervals for the difference between binomial proportions are evaluated. Computationally simpler methods are prone to a variety of aberrations and poor coverage properties. The closely interrelated methods of Mee and Miettinen and Nurminen perform well but require a computer program. Two new approaches which also avoid aberrations are developed and evaluated. A tail area profile likelihood based method produces the best coverage properties, but is difficult to calculate for large denominators. A method combining Wilson score intervals for the two proportions to be compared also performs well, and is readily implemented irrespective of sample size.

Binomial Distribution