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Biomedical subjects

S Kharb

Publications and source records attributed to S Kharb.

27 records · Page 2Linked to original sources
PubMed1999

Lymphocyte glutathione levels in acute leukemia.

The current interest in the clinical significance of the glutathione estimation in leukemia is due to its widespread effect on the cell constituent and cell function of the hematopoietic system. It helps and protects the cells against free radicals and reactive oxygen products. The present study was conducted to estimate glutathione levels in lymphocytes of 20 patients with acute leukemia before and after chemotherapy to observe the relation of glutathione level to response to chemotherapy. Twenty age and sex matched healthy volunteers served as control. In acute myeloid leukemia, the levels were almost double than that of controls (P<0.001). In acute lymphoid leukemia they were 2.5 times of the control. Lymphocyte glutathione levels was higher in active phase of disease than in remission. Lymphocyte glutathione level could act as a marker of leukemic activity and may help to predict onset of relapse. But, it is not only the determinant of response to chemotherapy.

Adolescent↗
PubMed1999

Protective function of alpha-tocopherol against the process of cataractogenesis in humans.

In a random trial 50 patients with unilateral/ bilateral idiopathic immature senile cataract (cortical n = 25, nuclear n = 25) requiring surgery at least in one eye were included in the present study. Reduced glutathione (GSH), vitamin E, malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were studied in these patients receiving either vitamin E (n = 12 in each subgroup) or placebo (n = 13 in each group) for 30 days. A comparable increase of vitamin E in both types of lens homogenates of cataractous patients was observed in the study group. The level of GSH in cortical cataractous lenses in the study group was significantly raised (p < 0.001) whereas it was not increased significantly in nuclear cataractous lenses (p > 0.05) as compared to the placebo group. The percentage decrease in MDA levels was greater in cortical cataracts (38.07%) than in the nuclear type (27.94%). The activity of GSH-Px in cortical lenses was higher than that in the nuclear cataractous lens. The change in the size of lens opacity in cortical cataractous patients receiving vitamin E therapy was significantly decreased as compared to the placebo group. There may be a direct modulating effect of vitamin E on some GSH-related enzymes and the cortex of the lens might be protected more than the nucleus.

Antioxidants↗
PubMed1999

Alpha tocopherol concentration in serum of critically ill patients.

OBJECTIVE: Alpha tocopherol is the biologically and chemically active form of vitamin E and is the most abundant lipid-soluble antioxidant in humans protecting the cell membranes. Serum concentration of the antioxidant alpha tocopherol was measured in critically ill patients in whom the excessive generation of reactive oxygen species could compromise antioxidant defence mechanisms. METHOD: Alpha tocopherol concentration in serum was measured spectrofluorometrically in critically ill patients admitted in intensive care unit and age matched healthy adults, patients with diabetes mellitus (DM) and chronic myeloid leukaemia (CML) served as control. RESULTS: Alpha tocopherol levels were significantly lower in critically ill patients (p < 0.001) as compared to healthy control subjects and those in two other disease groups (DM, CML) in which reactive oxygen species are reported to be increased. Elderly patients and patients who stayed longer than 8 days had lower plasma concentration of vitamin E. CONCLUSION: These findings indicate that antioxidant defence could be considerably compromised in these very sick patients and vitamin E supplementation may be beneficial in increasing their antioxidant reserve.

Adult↗
PubMed1999

Distribution of blood viscosity values and biochemical correlates in healthy adults.

OBJECTIVE: Increase in the viscosity of blood predict clinical manifestation of atherothrombotic vascular disease. The clinical utility of viscosity measurements in cardiovascular risk factor requires a reference value established from a healthy disease free population. METHODS: Blood viscosity (based on empirical Merill's formula), fibrinogen, haematocrit and lipid profile were measured in 100 fasting healthy nonsmoking adults (50 men, 50 women). RESULTS: The mean (+/- SD) blood viscosity values were 0.030 +/- 0.015 dyne/cm2. Men had higher viscosity values than women. Also, men had higher triglycerides, haematocrit as well as fibrinogen as compared to women. CONCLUSION: This study provides reference values for the blood viscosity, fibrinogen haematocrit and lipid profile. Viscosity measurements may improve identification and risk stratification of patients at high risk for atherothrombotic vascular disease and its complications.

Adult↗
PubMed1999

Immunological assessment in patients of leukaemia.

In the present study delayed cutaneous hypersensitivity response (DNCB test) and humoral response (by uantification of immunoglobulins) ware carried out in 20 cases of leukaemias. None of the cases was found to be anergic or immunodeficient. In remission also patients showed the normal response.

Adult↗
PubMed1998

Effect of adenotonsillectomy on arterial blood gases and acid-base balance.

Adenotonsillitis is quite common in children in India and untreated cases may land up with serious cardiopulmonary complications which promoted us to study arterial blood gases and acid-base balance in these patients. The study was conducted in 30 children with chronic adenotonsillitis and 30 age and sex matched controls. Arterialized capillary blood samples were taken before and 1 month after surgery while the subject was sleeping. Relative hypoxemia, rise in pCO2 and increased bicarbonates was observed in the study group, whereas change in pH was insignificant. The study showed that ventilatory impairment associated with adenotonsillar hypertrophy which manifests as hypoxia, hypercarbia and raised bicarbonate levels was reversed after adenotonsillectomy.

Acid-Base Equilibrium↗
PubMed1998

Lipid peroxidation and vitamin E levels in preeclampsia.

Twenty preeclamptic, 25 normotensive pregnant and 25 healthy nonpregnant women were analyzed with regard to serum malonaldehyde (MDA), vitamin E and lipid profile. In preeclampsia, MDA was significantly higher than control. MDA also showed high correlation to the level of blood pressure. Serum cholesterol and triglycerides were increased in normotensive women and still higher in preeclamptic women. A highly significant fall in vitamin E levels in preeclamptic women was noted as compared to control groups. Our study supports the view that an increased lipid peroxidation may be involved in vasoconstriction in preeclampsia.

Adult↗
PubMed1995

Vitamin E in intestinal fat malabsorption.

This study was done on 5 patients suffering from tropical sprue (8-10 years' duration). Serum vitamin E level was significantly low in these patients. Cell-mediated immune mechanism assessed by using 2,4-dinitrochlorobenzene, a skin-sensitizing hapten, was also depressed. Polyneuropathy was also observed. Repletion of vitamin deficiency was associated with marked improvement in T-cell functions and neuropathy.

Adult↗