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S Lanzafame

Publications and source records attributed to S Lanzafame.

At least 37 records · Page 2Linked to original sources
PubMed1995

Immunohistochemical expression of type VI collagen in superficial fibromatoses.

The expression of type VI collagen was studied immunohistochemically in 26 cases of superficial fibromatoses (palmar, plantar and penile) using an immunoperoxidase method for light microscopic visualization. The polyclonal antibody against type VI collagen used in this study was isolated from human placenta and its specifity was tested by immunoblotting assay. All cases consisted of multiple nodules showing a variable degree of cellularity and fibrosis. Depending on the predominant histological appearance of these nodules, each case was assigned to the three following phases: proliferative, involutional and residual. Morphologically normal palmar and plantar aponeuroses were included as controls. Immunohistochemical findings showed that type VI collagen was present as longitudinal thin fibers in normal palmar and plantar aponeuroses. A differential expression of this collagen was found in the different stages of superficial fibromatoses. Type VI collagen was markedly expressed as a distinct fibrillar network in the extracellular matrix (ECM) surrounding proliferating stromal cells in proliferative and involutional phases. Its expression completely disappeared from the connective tissue undergoing fibrotic transformation during involutional and residual phases. The results of the present study suggest that type VI collagen is an extracellular marker of stromal tissue proliferation and is involved in the early phases of tissue remodelling occurring in the superficial fibromatoses.

Collagen↗
PubMed1994

Expression and distribution of type VI collagen in gynecomastia.

We investigated the distribution of type VI collagen in 36 cases of routinely fixed and paraffin-embedded gynecomastia using an immunoperoxidase method for light microscopic visualization. Four samples of normal male mammary gland tissue were also included as controls. A protease predigestion was essential for the visualization of this extracellular matrix (ECM) glycoprotein. In normal male breast, no immunoreaction for type VI collagen was detected in the stroma surrounding the ducts. Gynecomastia was classified into three histological types: florid (type I), fibrous (type II), and intermediate (type III). Type VI collagen was differentially expressed in the periductal stroma of all types. This collagen was markedly expressed at the early disease stage (type I) when the periductal stroma is highly cellular and vascular. Its expression decreased when periductal stroma undergoing fibrotic transformation (type III) and completely disappeared from the dense periductal stroma of fibrous stage (type II). These findings suggest that type VI collagen is involved in the ECM remodelling occurring in gynecomastia.

Adult↗
PubMed1994

Histologic patterns of omeprazole and nizatidine-healed duodenal ulcers:accuracy of endoscopic diagnosis.

The mechanisms underlying duodenal ulcer (DU) recurrence after endoscopically confirmed healing are unclear. We sought to examine histologic differences in healing induced by omeprazole and nizatidine. This also entailed assessing interobserver variation in endoscopic diagnosis and the correlation between endoscopic and histomorphologic healing. We treated 31 DU patients for 4 weeks with either omeprazole (20 mg daily a.m.) or nizatidine (300 mg twice daily). The healing rates of both groups showed no significant differences (86.7% versus 81.2%; p = 0.5). Good mucosal repair rates did not differ significantly (38.5% versus 69.2% respectively; p = 0.5). Endoscopists' agreement over scar type was 0.80, with the chance of agreement 0.70 (k = 0.34 +/- -0.08). The correlation between macroscopic and histologic appearance of scars was fair, but fully significant (r = 0.48; p < 0.05). We conclude that the study was too small to detect significant differences in healing patterns between the two drugs. The wide variation in endoscopic diagnosis suggests that mucosal repair is best assessed by histologic examination of biopsy samples.

Adult↗
PubMed1994

Immunohistochemical staining patterns of type VI collagen in the normal, hyperplastic, and neoplastic adult male breast.

Ten cases of primary invasive male breast ductal carcinomas (N.O.S.), one case of male breast fibroadenoma, seven cases of pure florid gynecomastia and four cases of normal mammary glands were examined immunohistochemically for type VI collagen expression and distribution. In normal male breast type VI collagen was absent in the whole fibrous stroma. On the contrary this collagen was markedly expressed in the stroma of invasive carcinoma as well as fibroadenoma and gynecomastia. In all these proliferative diseases, type VI collagen gave a distinct basement membrane-like deposition at the epithelial-stromal junctions while organized a fibrillar network in the interstitium. Our immunohistochemical findings showed that type VI collagens is involved in the stromal response of benign and malignant male breast proliferative diseases.

Adult↗
PubMed1994

Minimal breast cancer: analysis of proliferative activity using a monoclonal antibody to proliferating cell nuclear antigen (PCNA) and its relationship to histological grade and prognosis.

Sixty-five cases of invasive breast cancer < or = 1 cm. in largest diameter (pT1a-b) were studied retrospectively using immunohistochemical staining with PC10, a monoclonal antibody to proliferating cell nuclear antigen (PCNA). The percentage of PC10 positive tumor cells was closely related to histological grading. No association was found between PC10 score and nodal status. ER-ICA was performed on 42 cases and showed no correlation with PC10 staining. The clinical behaviour of these tumors was excellent, with 5-year survival rates overall of 96% (90% disease free survival), and apparently unrelated to histological type and grade, nodal involvement and hormonal receptor status. The prognostic value of PCNA labeling rates remains nuclear in breast cancer of minimal size as well as in larger ones.

Adult↗
PubMed1989

[Esophageal stenosis caused by benign mucous pemphigoid].

The Authors report a case of benign mucous pemphigoid. The review of the literature has confirmed the rarity of this disease. After having discussed acquired diagnostic data, the Authors show reasons to justify the endoscopic surgical treatment. Furthermore, they underline the importance of associated medical therapy as well as follow-ups which patients must periodically undergo.

Aged↗
PubMed1988

Rectal carcinoid tumors. Report of two cases.

Two cases of rectal carcinoid tumors are reported. In the first case the endoscopic diagnosis was intramural rectal neoformation. At histology, rectal carcinoid tumor with infiltration of the muscularis mucosae was diagnosed. Abdominoperineal resection was performed. The patient is still living 3 years following surgery. In the second case, the endoscopic diagnosis was non-adenomatous polyp. Endoscopic polypectomy was performed. At histology, rectal carcinoid tumor was diagnosed. The patient is still living 2 years following surgery.

Carcinoid Tumor↗
PubMedDate not supplied

Correlation between intestinal metaplasia of the gastric cardia and gastroesophageal reflux disease.

BACKGROUND/AIMS: IMC (intestinal metaplasia of the cardia) has been a subject of great interest, given the rapidly increasing incidence of adenocarcinoma in this location, over the past two decades. Whether this histological alteration is a consequence of gastroesophageal reflux disease, or a manifestation of an H. pylori-related multifocal atrophic gastritis, is unclear. Furthermore, whether IMC should be considered a premalignant lesion of gastric cardia is still unknown. We performed a prospective study in order to determine the prevalence of IMC in patients presenting for elective esophagogastric-duodenal endoscopy and to evaluate a potential association between IMC and some clinical, endoscopic and histological variables. METHODOLOGY: Biopsy specimens were taken from 105 unselected patients undergoing routine diagnostic endoscopy. Eight biopsies were taken from different sides, for histological evaluation: 1 above and 3 below the squamocolumnar junction, 2 from gastric fundus and 2 from gastric antrum. All specimens were stained with hematoxylin and eosin-Alcian blue and modified Giemsa to facilitate the detection of H. pylori. RESULTS: Eighty-six patients (50 males, 36 females) with a mean age of 46.5 years (range: 23-75 years), were included in the study. Twenty-one (24.5%) were found to have IMC; 4 (19%) of these had concomitant low-grade dysplasia of the cardiac mucosa. IMC was associated with: males (P = 0.04), endoscopic diagnosis of esophagitis (P = 0.02), histological diagnosis of esophagitis (P = 0.008), mucosa of the cardiac type (P = 0.02), chronic carditis (P = 0.002) and dysplasia (P = 0.04). There was no correlation with: age, reflux symptoms, activity of carditis, H. pylori infection of the cardia and intestinal metaplasia of the distal gastric mucosa. CONCLUSIONS: IMC is common in our area. It is associated with endoscopic and histological changes of gastroesophageal reflux disease, but not with H. pylori infection of the cardia. Although 19% of patients presented concomitant dysplasia (of low grade), long-term follow-up studies will be necessary to assess the effective risk of IMC for cancer transformation.

Adult↗
PubMedDate not supplied

A pure primary squamous cell breast carcinoma presenting as a breast abscess: case report and review of literature.

We report the fourth worldwide case of pure primary squamous cell carcinoma of the breast presenting as an abscess. An inflammatory breast lesion in postmenopausal woman must be suspected as a malignant one and drainage of the abscess has to be followed by an accurate excision. The finding of a pure squamous cell carcinoma bears the necessity of an accurate diagnostic work up, to exclude a skin lesion or a metastasis from other district. Also histological criteria are discussed. More difficult to outline therapeutic options, due to the rarity of disease and great difference in outcome observed.

Abscess↗
PubMedDate not supplied

A case of branchial cyst with an ectopic thyroid papillary carcinoma.

We report the fifth case worldwide described of thyroid papillary carcinoma arising in a branchial cyst. A metastatic lesion from occult primary thyroid carcinoma has to be ruled out. The embryology of this cervical lesion is also discussed in order to point out the diagnostic criteria and the therapeutic guidelines.

Adult↗
PubMedDate not supplied

[Molecular characterization of cutaneous neuroendocrine (Merkel cell) carcinoma. Review of the literature and presentation of a caseload].

Molecular characterization of neuroendocrine (Merkel cell) carcinoma of the skin. Review of the literature and report of three cases. Although neuroendocrine carcinoma of the skin (NECS) is comparatively a rare clinical-histological entity, numerous morphological and ultrastructural studies have been carried out since the tumor was identificated by Toker (1972). Recently immunocytochemistry has allowed a better molecular characterization (immunophenotype) of this tumor and a more exact diagnosis. The main problem for the pathologist is the differential diagnosis between NECS and skin neoplasms--both primitive and metastatic--which require a more aggressive treatment. Often the classical morphological criteria do not distinguish NECS from non-Hodgkin's lymphoma, amelanotic melanomas, cutaneous metastases of lung small cell carcinoma or of neuroblastoma. The co-expression of cytokeratins and neurofilaments constantly found in NECS, is surely the best differential criterion from non-neuroendocrine carcinomas. Furthermore, the typical paranuclear location of both the intermediate filaments in NECS is a distinctive peculiarity as opposed to lung microcytoma, where cytokeratins and neurofilaments, when present, show widespread perinuclear positivity. Chromogranin A is found only in a small percentage of tumor cells, whilst synthesis of calcitonin, somatostatin, gastrin, ACTH, is very rare. Finally, the lack of common leukocyte antigen (CLA), S-100 protein and vimentin in NECS rules out the diagnoses of lymphoma, melanoma and sarcoma respectively.

Aged↗
PubMedDate not supplied

[Cerebral gliosarcoma. On the possible pericytial origin of the sarcomatous component].

We have reported two cases of cerebral gliosarcoma by immunohistochemical method. The antibodies anti S-100, anti GFAP and anti vimentin have supported the presence of glial and mesenchymal elements. Factor VIII has labeled the endothelium of the newly formed vessels. Collagen IV has shown atypical cells in the thickness of the vascular walls, and ribbons of adventitial cells throwing in the surrounding parenchyma. By these results, the pericitic origin of the sarcomatous elements could be hypothesized.

Brain Neoplasms↗