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S Leodolter

Publications and source records attributed to S Leodolter.

At least 91 records · Page 5Linked to original sources
PubMed1990

[Catamenial pneumothorax--endometriosis as a multidisciplinary challenge].

While the occurrence of heterotopic endometrial tissue is relatively common during the reproductive years, involvement of the pulmonary pleura is an extremely rare event. Since the condition is poorly understood, patients with "catamenial pneumothorax" tend to be subjected to often quite stressful surgical procedures. These do not provide a permanent cure, as shown by the presented case. Traditional hormonal regimens are also associated with high relapse rates. Consequently, abdominal hysterectomy with bilateral removal of the adnexa has been the treatment of choice if fertility was no longer desired. The battery of conservative treatment modalities has, however, recently been expanded by the use of GnRH analogues and antigestagens. As embryogenesis and the factors underlying the development of endometriosis are better understood and as the complex symptoms of the condition as well as the state-of-the-art therapeutic approaches are more widely appreciated, women afflicted with the condition should be able to benefit from a more rational and possibly even causal treatment concept.

Adult↗
PubMed1990

[Intravaginal culture--simplification of IVF].

Initial results with intravaginal cultures (IVC) of human oocytes are reported. Up to 5 oocytes are harvested by follicular puncture guided by transvaginal sonography. Together with washed spermatocytes (final concentration 0.1 to 0.15 x 10(6)/tube) these are transferred to a plastic capsule filled with Menezzo B2 medium. After air-tight closure the capsule is placed into the patient's vagina and left in situ for 48 to 52 hours. Capsule loss is prevented by plugging the vagina with a tampon of appropriate size. In the first 15 females undergoing IVC fertilization was obtained in 56% of cases. Three intact pregnancies were recorded after embryo transfer. Of these, 2 occurred in the group of patients with tubal infertility and one in the group with tubal infertility and an additional andrologic abnormality. Our results and the reports by others suggest that IVC may well facilitate the technique of IVF. Potentially detrimental effects of light exposure and temperature variations on the gametes during conventional IVF are minimized. The more active involvement of the patients is an added psychological benefit.

Cells, Cultured↗
PubMed1990

Determination of HER-2/neu amplification and expression in tumor tissue and cultured cells using a simple, phenol free method for nucleic acid isolation.

A rapid, simple and non-toxic procedure for the simultaneous isolation of DNA and RNA from tumor tissue and cells grown in vitro is described. Guanidinium isothiocyanate was used for homogenization of tumor tissue and for cell lysis. Separation of proteins, DNA and RNA was carried out by isopycnic centrifugation in cesium trifluoroacetate. DNA was further purified by salting out residual protein. Nucleic acids prepared by this method from 47 primary human carcinomas and 17 human cell lines were analysed for amplification and expression of the HER-2/neu proto-oncogene. 2- to 10-fold amplification of HER-2/neu was noted in 7/22 mammary carcinomas (32%) and in 4/14 ovarian carcinomas (28%). No amplification of the proto-oncogene was found in 4 laryngeal carcinomas, 1 pharyngeal carcinoma, 2 retrolingual carcinomas, 3 gastric carcinomas and 1 kidney carcinoma. HER-2/neu overexpression was observed in 6/22 of mammary carcinomas (27%) and 7/14 of ovarian carcinomas (50%). No overexpression was found in all other carcinomas studied. Concordance between amplification and overexpression was noted in 3 mammary and 4 ovarian carcinomas, respectively. 3 mammary and 3 ovarian carcinomas showed overexpression without amplification. 5 human mammary carcinoma cell lines showed both amplification and overexpression of HER-2/neu. In two mammary carcinoma cell lines (MDA MB-453 and ZR 75-1) overexpression was noted without amplification of the proto-oncogene. These data combine to suggest that mechanisms other than gene amplification may also lead to overexpression of the HER-2/neu protooncogene in cancer cells.

Blotting, Southern↗
PubMed1989

Arbaprostil's [15(R)-15-methyl PGE2] effects on intrauterine pressure in the nonpregnant and pregnant human female--a report of four clinical trials.

Four clinical trials evaluating arbaprostil's effects on the human uterus are reported. The initial two trials measured intrauterine pressures in nonpregnant and pregnant human females following arbaprostil doses of 10, 25, and/or 50 mcg. No statistical differences were found at any dosage level in either study for average uterine resting pressures, average peak pressures, the number of contractions or Montevideo units. Subsequently, two trials determined the abortifacient potential of arbaprostil in pregnant women during the first trimester. The first utilized total daily doses of 400 and 800 mcgs. while the second used total daily doses of 1200 and 1600 mcgs. Vaginal spotting was noted in one woman receiving 400 mcgs, three receiving 1200 mcgs. and in two receiving 1600 mcgs. One episode of moderate bleeding was seen in the latter study. Based on these studies, arbaprostil exhibits little potential for inducing abortifacient activity at these dosages in these patient populations.

Arbaprostil↗
PubMed1989

[Treatment of tubal pregnancy with prostaglandins: a multicenter study].

In this prospective multi-centre-study, the new treatment of tubal pregnancies by means of local prostaglandin (PG) F2 alpha and systemic PG E2 application was compared to the usual surgical technique of eliminating the conceptus. In 71 patients treated with PG, the method proved to be successful in 81%; 21 patients (19%) required surgical intervention later. With an initial beta-hCG level of 2500 mIE/ml, the success rate increased to 88%. The duration of hospitalisation was significantly reduced in the PG group compared to the patients treated by primary operation (3 +/- 1 versus 6 +/- 2 days). In the PG-group, only 2 of 24 hysterosalpingograms showed tubal occlusions after treatment, whereas occlusion was present in 3 of 8 patients of the surgically treated group. Four subsequent intrauterine pregnancies in the PG-group occurred compared to none in the surgical group. PG treatment of tubal pregnancy in patients with a low initial beta-hCG value (less than 2500 mIE/ml) revealed promising results with regard to reduced postoperative morbidity and future fertility.

Abortifacient Agents↗
PubMed1988

A comparison of fluconazole and ketoconazole in the oral treatment of vaginal candidiasis; report of a double-blind multicentre trial.

One hundred and eighty three patients with vaginal candidiasis were randomly allocated to treatment with either fluconazole in a single oral 150 mg dose, or ketoconazole, 200 mg twice daily for 5 days. Favourable clinical responses were obtained in 92% of the patients in the fluconazole group and in 89% of those in the ketoconazole group after 5-16 days. Long-term evaluation at 27-62 days showed favourable clinical responses in 86 and 88%, respectively. Candida was eradicated from the vagina in 77% of both treatment groups at the long-term evaluation. The relapse or reinfection rate was similar for both groups, ranging between 4-8%. One hundred and thirty two of the 160 patients who had rectal swabs cultured for yeast at baseline, gave positive results. Of the 27 patients in the fluconazole group whose rectal cultures remained negative at the long-term evaluation, 26 maintained mycological cure of their vaginal candidiasis. In contrast, patients with positive rectal cultures at this time were much less frequently associated with mycological cure. The results were similar for the patients in the ketoconazole group. Treatment-related side effects in both groups were few and minor. This double-blind multicentre study showed that a single oral 150 mg dose of fluconazole was as effective as 5 days of oral ketoconazole medication in the treatment of vaginal candidiasis.

Administration, Oral↗
PubMed1988

[Hydronephrosis as a late complication in gynecologic neoplasms: current possibilities for sensible urinary drainage].

By means of new endourological techniques supravesical urinary drainage is possible with minimal invasiveness. In 10 patients suffering from hydronephroses caused by gynaecological malignancies a worthwhile prolongation of lifetime was achieved by palliative urinary drainage (mean follow-up: 9.1 months). Four cases with fistulas and consecutive incontinence were treated successfully by percutaneous ureteral occlusion (3x), inner stenting respectively (1x). The very low invasiveness of the methods described should encourage the decision towards palliative supravesical urinary drainage.

Aged↗
PubMed1987

[Stimulation of prolactin secretion by vasoactive intestinal peptide].

Dopamine has already been recognized as an inhibiting factor of prolactin. On the other hand, the stimulators of prolactin are still judged diversely at the moment. In addition to hypothalamic TRH, serotonine as well as endogenous opiates and VIP are under discussion. The object of our studies was testing the function of VIP in directing prolactin secretion on three different groups of patients: 1. hyperprolactinaemic women of WHO-group VI; 2. patients who had undergone a TRH-test along with an endocrine check-up; 3. post partum in childbed. The determination was made from analyzing peripheral blood by radioimmunoassay. In 20 amenorrhoeic patients of WHO-group VI, their prolactin and VIP levels were determined between 8 and 10 a. m. Only 4 patients showed a VIP-concentration below 20 pg/ml (which represents the limit of detectability). The other 16 patients showed an increased concentration of VIP. In 15 cases there was an obvious correlation of VIP and prolactin concentrations according to the linear regression. In the course of a TRH-test to 20 and 40 minutes after initial blood examination prolactin and VIP were again determined. After 20 minutes all patients showed a threefold increase of the initial prolactin value. On the other hand, VIP increased in 3 cases only. Apart from all those patients who wanted to become pregnant, these three women--being postmenopausal--showed increased initial values already in the unstimulated condition. The third group of patients consisted of 26 women in childbed. Their prolactin and VIP were determined between the second and eight day post partum.(ABSTRACT TRUNCATED AT 250 WORDS)

Female↗
PubMed1985

Prostacyclin does not influence placental blood pool in vivo.

In 20 healthy pregnant women placental blood pool was estimated by means of placental scintigrams before, during and after infusion of either prostacyclin (PGI2) or placebo for 10 min to study the effect of PGI2 on the local regulation of uterine blood flow. There was no difference in the mean number of counts between the PGI2- and the placebo-treated group. Therefore no effect of PGI2 on uteroplacental blood pool could be detected in healthy gravidae.

Adult↗
PubMed1983

[Theory and practice in the care of diabetic pregnancy--a timely concept].

Perinatal mortality in diabetic pregnancy has decreased markedly over the past 25 years; this reduction in fetal loss can be attributed to a variety of advances. These include technologic advances in obstetrics and pediatrics, as well as greater understanding of the metabolic aberrations occurring in pregnancy complicated by diabetes. Basically it seems convenient to consider the various disorders in cases of diabetes in pregnancy from several points of view: 1. the effect of pregnancy on the maternal diabetes and 2. the effect of maternal diabetes on the pregnancy and especially on the fetus, and from the above results we can consider 3. an actual procedure in the care of diabetic pregnancy. Regarding the necessary criteria in the care of pregnant diabetics neither pregnancy nor delivery will cause any serious problems.

Blood Glucose↗