PubMed Health⌕ Search

Biomedical subjects

S Liebe

Publications and source records attributed to S Liebe.

At least 73 records · Page 4Linked to original sources
PubMed1990

[Enzymatic determination of glandular kallikrein in human pancreatic secretions].

A method for the enzymatic determination of tissue kallikrein (EC 3.4. 21.8) in human pancreatic juice using D-Val-cyclohexyl-Ala-Arg-4-nitroaniline as substrate as well as the reaction conditions optimized for 37 degrees C are described. The reaction is characterized by the following kinetic parameters, determined according to Hanes and Eisenthal, Cornish-Bowden: KM = 2.10(-5) mol/l; Vmax = 0.800 mumol/(l.s). The coefficient of intra-assay variation (N = 10) was determined to 2.54%. The detection limit of the assay was found to be 8.63 nmol/(l.s).

Clinical Enzyme Tests↗
PubMed1989

[Transpapillary endoprostheses in malignant and benign bile duct diseases].

During the last years the non-surgical drainage of the bile ducts developed to an efficient treatment method and is a real enrichment in the concept of treatment of the malignant obstruction of the bile ducts. In no more radically operable carcinomas in the area of the bile ducts, the pancreas or the porta of the liver it is to be preferred to a palliative drainage operation. In case the drainage of the bile ducts shall not be used therapeutically (through-drainage), an inner drainage in form of an endoprosthesis should always be preferred. As a procedure of the 1st choice the endoscopic retrograde technique is recommended, as procedure of 2nd choice the percutaneous transhepatic technique. Benign basic diseases are more infrequently the reason for the insert of a bile duct drainage. The main indications are general inoperability in non-extractable calculi in the common bile duct. In our own cases patients could be followed up up to 3.5 years. No complications were observed. The occlusion of prostheses does apparently not play a role.

Ampulla of Vater↗
PubMed1989

[Endoscopic perfusion manometry: diagnosis of functional biliary disorders].

During the last years the endoscopic perfusion manometry developed many new recognitions about the courses of pressure and motility at the sphincter Oddi. The clinical and experimental application of the method concentrated itself to the proof of functional disturbances. Many results are contradictory and a whole series of questions is unsolved. The own investigations concentrated themselves on pressure measurements in the common bile duct in patients with removed gallbladder, in whom by comprehensive diagnostic measures no organic disease of the biliary tract could be found. Two groups of patients were compared. Patients of group 1 (n = 14) had no biliary symptoms after cholecystectomy. The patients of the second group (n = 20) continued to have biliary complaints after cholecystectomy, or they again occurred after symptom-free interval. At the beginning of the investigation the pressure in the common bile duct was the same in the two groups. In group 1 the pressure remained constant also during the manometry and the patients remained without any complaints. However, in group 2 a gradual increase of pressure and biliary complaints developed during manometry. These occurred after an average pressure increase of 8 Torr. The velocity of the increase of pressure well correlated with the intensity of the complaints. The symptoms during manometry were identical with those cited in the anamnesis. The pressure increase in the common bile duct is regarded as an expression of a functional disturbance of the sphincter of Oddi. The constant perfusion rate (1.3 ml/min) in this group of patients is sufficient as volume load, in order to detect a disturbed drainage capacity of the papilla. On its part the increase of the pressure is responsible for the evocation of the complaints.

Ampulla of Vater↗
PubMed1989

[Biliary pancreatitis--pathogenesis, therapy, results].

According to current knowledge biliary (gallstone-associated) acute pancreatitis is induced by transient obstruction of the papilla by migrating gall stones. It seems, therefore, rational to remove the occluding stone as early as possible by endoscopic papillotomy (EPT). Uncontrolled studies have shown that patients with acute pancreatitis are not more endangered by EPT than those without pancreatitis and that the intervention seems to beneficially influence the course of the pancreatic disease. Early endoscopy (within 48 h), however, revealed incarcerated papillary stones only in 10% but common bile ducts free of stones in 24%. There seems to be only a small subgroup with acute biliary pancreatitis who might benefit from early EPT. This subgroup should be characterized more precisely. Early EPT indiscriminately performed in any kind of acute pancreatitis has little rational ground and is, up to now, not justified.

Acute Disease↗
PubMed1987

[Studies of pure human pancreatic juice].

The test-combination from Fa. Boehringer Mannheim GmbH for measuring citric acid present in foodstuffs is also suitable for use in human pancreatic juice. Accuracy, within-run and between-day imprecision are satisfactory. The detection limit of the method is at least 10 mumol citrate/l pancreatic juice. The limit can be reduced when exact additions of citrate are given to the pancreatic juice and then evaluated using regression analysis. The presence of Ca-ions and possibly also trypsin in the material to be tested does not interfere with the reaction. It is, however, necessary to remove proteins from the sample. Deproteinization can be performed either by ultrafiltration or with perchloric acid.

Cholecystokinin↗
PubMed1986

[Pure human pancreatic juice. Methodologic aspects of pancreatic juice collection and interpretation of findings].

A procedure to collect pure pancreatic juice endoscopically is described which allows an instant estimation of the protein content of the secretion by measuring its absorption at 280 nm. By this the kinetics of protein secretion can already be read from a curve during the collecting procedure, thus avoiding a multitude of small fractions otherwise necessary. Timing and size of the samples are adjusted individually according to the course of protein secretion and not to a rigid regimen. The method described is especially apt to investigations of pancreatic proteins and substances the secretion of which parallels that of protein; e. g. trypsinogen, calcium and zinc, however not sodium and potassium.

Cholecystokinin↗
PubMed1986

[Endoscopic perfusion manometry of the common bile duct in the post-cholecystectomy syndrome].

In 14 cholecystectomized patients with recurrent attacks of pain endoscopic manometry of the c.b.d. was carried through after thorough exclusion of organic diseases. Two different conspicuous types of pressure behaviour became evident: In 8 pat. the pressure in the c. b. d. increased steadily during perfusion and finally triggered pain, identical to the spontaneous one according to localization and character. In 6 pat. even after prolonged perfusion no pain emerged and the c. b. d.-pressure remained unchanged. As the increase in c. b. d.-pressure connected with provocation of pain was reproducible, perfusion manometry in the c. b. d. seems to be a mean of delimitating and objectifying functional disturbances of the bile duct.

Cholecystectomy↗
PubMed1985

[Transpapillary bile duct endoprostheses in obstructive jaundice].

12 patients were treated with endoscopic transpapillary biliary endoprosthesis. The indication for biliary drainage was malignant obstructive jaundice in 10 and benign obstructive jaundice in 2 cases. The effectiveness of drainage is indicated by the disappearance of jaundice. The lifetime of the endoprosthesis was maximal 4 months and the survival of the tumor patients maximal 6 months. If the endoprosthesis is occluded replacement by a new one is necessary. Nonsurgical biliary drainage is an alternative method to palliative operation and should be preferred in general inoperability and in patients with irresectable tumors.

Ampulla of Vater↗
PubMed1984

[Diseases caused by sutures following stomach operations--yes or no?].

The importance of remaining suture material in the operated stomach was studied on two groups of patients. Group I consisted of 560 patients who were found among about 6,000 gastroscopically examined patients. Group II consisted of 102 non-selected patients who underwent a gastric operation and in certain intervals were repeatedly examined gastroscopically and clinically after operation. The most frequent gastric operation was Billroth II, followed by Billroth I and the selective proximal vagotomy with pyloroplasty. Group I shows that the proof of suture material in the operated stomach depends on the postoperative time interval. During the first postoperative year the proof is most frequent with 48.5% and permanently decreases in the following years. After the 15th postoperative year no threads were found. Suture material could be more frequently proved in the Billroth I stomach (52.5%) than in the Billroth II stomach (21.7%). In group II in 56% of the patients suture material was found in the stomach. Also here the Billroth I stomach was at the top. The analysis of the complaints in patients with and without suture material resulted in the fact that remaining suture material is responsible for epigastric complaints and complications such as ulcer and haemorrhage. The notion of "thread disease" is justified. The frequency of the symptomatic thread disease during the first two years after operation is 30%. A plan of measures for the decrease of this complication is proposed which among others provides the use of absorbable suture material for all sutures at the stomach and demands the endoscopic removal of all rests of sutures.

Follow-Up Studies↗
PubMed1984

[Polypectomy of the stomach and colon. 6 years' analysis].

From August 1976 till December 1982 855 polyps were removed by endoscopic polypectomy from the upper and lower digestive tract in 450 patients. With 0.70% the complication rate was very low. Altogether 6 haemorrhages were observed, three of which had to be treated by a transfusion. There did not occur neither a complication which had to be treated surgically nor a fatal one. Among the colon polyps in 9% and among the gastric polyps in 2.9% a malignant change was found. The macroscopic picture of a polyp does not allow a conclusion to the histological findings. Therefore the endoscopic polypectomy and the careful histological investigation of the polyp demand a close cooperation between specialist in endoscopy and pathologist.

Colonic Polyps↗
PubMed1984

[Long-term observation of patients with gastric polyps].

104 Patients with gastric polyps and 8 patients with gastric polyposis were controlled between 1 and 7 years after polypectomy. In about 20% of the cases recidivations of polyps were found. Patients with primarily multiple polyps had more frequently recidivations than patients with solitary polyps. The recidivation polyps are in most cases small and histologically above all hyperplastic. In 4 cases a carcinoma of the stomach developed after 1-4 years. In patients with polyposis of the stomach no development of the carcinoma was observed. Patients with polyps of the stomach and polyposis of the stomach should annually be examined endoscopically.

Female↗
PubMed1984

[Use of ultrasound tomography in the control of endoscopic retrograde bile duct drainage].

The sonography of three patients with a transpapillar catheter within the bile duct shows typically findings as the picture of catheter as a tubular structure with high level reflectivity, reduction of bile duct calibers and air in the bile ducts. The diminuation of the bile duct calibers is an argument for normal transpapillary bile flow and occurs before the change of laboratory parameters. In case of jaundice the differentiation between metastatic tumours of the liver and occlusion of the catheter is possible on the basis of ultrasound. After the diminuation of calibers of the bile ducts a metastatic tumours of liver are better to be seen.

Cholestasis, Extrahepatic↗
PubMed1984

[Localization of colon adenomas in relation to age and sex. A colonoscopy study].

The material consists of 318 colonic adenomas in 162 patients - 78 women and 84 men. Patients with partly colonoscopy, polyposis or additional carcinoma are not involved. Distribution of adenomas is influenced by age, sex, and the number of polyps per patient. With increasing age the number of adenomas increases in the proximal parts of the colon. In contrast the number of polyps decreases in the rectum. This change in distribution is caused by age itself and by a greater number of patients with multiple adenomas, in which the number of polyps in proximal parts of the colon is increased independent of age. Women have significant more frequently solitary polyps than men and the rectum is much less involved. There are two parallels between adenoma-disease and carcinoma-disease of the large intestine: With increasing age the number of colonic carcinomas increases in the proximal parts of the large intestine and the rectum is also less involved in women.

Adenoma↗
PubMed1983

[Diagnostic value of direct and indirect cholangiography - a comparative study].

Stimulated by Goodman's paper and the discussion evoked by this we, too, compared the valency of the intravenous cholography with the evidence of the direct cholography. Among our selected patients (95 patients) in 60% we did not find an accordance of the two methods. The intravenous cholography were with Goodman' criteria judged by a radiologist at 42% as optimal, at 24% as suboptimal and at 34% without evidence. Also a control group of 50 intravenous cholographies in accidental order were to be estimated as optimal only in 56%. In 39 cases only the direct cholography could bring the definitive diagnosis, the intravenous cholographies, no doubt, were pathologically sufficient, but not for the final clarification. 16% falsely positive and 10% falsely negative judged intravenous cholographies are particularly aggravating in our study. Mainly the explanation of a dilated choledochus and changes near the papilla, the finding of the calculus in the duct system and the possibility to estimate the intrahepatic bile ducts were the weak points in the evidence of the intravenous cholography. According to our results only the direct cholography brings the exact and for therapy guiding diagnostics, particularly before reinterventions on the bile ducts with the increased risk of operation. This and the justifiably small complications vindicate the invasive diagnostics.

Biliary Tract Diseases↗
PubMed1983

[Endoscopic papillotomy in East Germany].

Through a survey 751 patients, in whom an endoscopic papillotomy (EPT) had been tried, were registered to the end of 1981. EPT was technically successful in 695 cases (92,5%). Control investigations were available for 650 patients, the aim of therapy being achieved in 594 cases (91,4%). The main indication was choledocholithiasis, 90,2%--among them 10% with gallbladder in situ--followed by benign papillary stenosis without stones (5,9%), carcinoma of the Vaterian papilla (3,2%) and rare indications such as ascaridiasis, choledochocele and stones of the pancreatic duct (0,7%). 53 patients suffered from more serious complications (7,6%), 18 had to be operated on (2,6%), and 7 patients died due to EPT (1%). The results are in agreement with those of other statistics from various countries.

Ampulla of Vater↗
PubMed1982

[Activation of the antihemophilic globulin A (factor VIII). 3. Activation by servalyte].

A series of ampholytes is investigated concerning its influence on the activity of factor VIII. While positively charged ampholine (polyamino-polycarboxylic acids) may increase the activity of factor VIII, positively charged amino acids and dipeptides do not have this property. A group of substances (servalyte) constructed similarly to ampholine which besides amino groups and carboxyl groups has as charge carriers also phosphate and sulphate groups increases the activity of factor VIII like ampholine. Obviously not only the net charge of the molecule plays an essential role in the influence on the activity of factor VIII, but also size and configuration.

Blood Coagulation Tests↗