PubMed Health⌕ Search

Biomedical subjects

S Liebe

Publications and source records attributed to S Liebe.

At least 91 records · Page 5Linked to original sources
PubMed1982

[Activation of antihemophilic globulin A (factor VIII). 1. Activation by ampholine].

In vitro ampholine leads to an activation of the antihaemophilic globulin A (factor VIII). The effect is bound to the positive charge of the ampholine molecule. Negatively charged molecules of the same structure do not have such an effect. The increase of the activity of factor VIII depends on the ampholine concentration and is possible about twice as much. In great ampholine concentrations the factor VIII is again inhibited. The plasma of patients with haemophilia A and of patients with v. Willebrand's disease does not show any differences in the increase of activity. The activation is no time-depending reaction, but begins at once.

Ampholyte Mixtures↗
PubMed1982

[Activation of the antihemophilic globulin A (factor VIII). 2. Effect of ampholine on the course of blood coagulation and the molecular conformation of factor VIII].

Ampholine leads to an increase of activity of factor VIII and in vitro at the same time inhibits the activation of factor XII. The influence on factor XII takes place via influence on the contact surface. All the other coagulation factors remain uninfluenced. There are no differences between normal plasma and plasma of haemophilia with regard to the influence by ampholine. The molecular weight of factor VIII remains uninfluenced by ampholine. The activity of the various molecular forms of factor VIII is increased at the same level by ampholine.

Ampholyte Mixtures↗
PubMed1982

Immunological reactivity to soluble tumour extracts (STE) in gastrointestinal tumours and related risk groups.

One of the mose important demands on tumour diagnosis is the detection of early and recurring cancer. Recently, immunological tests have revealed possibilities of meeting these expectations. By means of the leukocyte adherence inhibition test (LAI) as tube test we investigated the immunoreactivity of leukocytes of leukocytes to STE in patients with colonic and gastric carcinoma, and the risk groups of Biermer's disease and colonic polyps. As control groups we used 30 blood donors and 37 patients with non malignant colonic diseases. The detection of autoantibodies with the indirect immunofluorescence technique and immune complexes supplemented the immunological investigations. The LAI and STE used are suitable for the diagnosis of gastric and colonic cancer with a rate of accuracy of more than 90 per cent. In the risk groups of colonic polyps the LAI offers a good possibility of detecting early carcinomas, and of monitoring the successful removal of polyps. Problems of autoimmunity in Biermer's disease and colitis ulcerosa do not allow a definite decision regarding a malignant stage in LAI positive patients.

Adult↗
PubMed1981

[Patient with stomach polyps--a risk group for colorectal tumors? Haemoccult studies on 106 patients].

106 patients in whom from 1 to 4 years ago a gastric polyp had been removed were examined for occult blood in the feces (haemoccult test). It should be tested whether in carries of gastric polypi increasingly appear colorectal tumors. Altogether 15 patients with colorectal tumours were found among the 106 carriers of gastric polypi. In 11 patients a removal of an adenoma from the colon was performed already before the haemoccult-examination. With the help of the haemoccult-examination 2 carcinomas of the colon and 2 carriers of adenomas were newly recognized as well as 2 recidivations of adenomas. Carriers of gastric polypi should be regarded as risk group for colorectal tumours. The dispensary care must be extended not only to the stomach but also include a careful anamnesis with regard to colorectal tumours and the search for occult blood in the feces.

Colonic Neoplasms↗
PubMed1981

[Intraluminal diverticulum of the duodenum].

The intraluminal diverticulum of the duodenum is a rare disease. Up to now about 50 cases were described. The case in question is a saccate evagination of the duodenal mucous membrane near Vater's papilla. The diverticulum lies within the duodenal lumen and inside and outside it is coated by duodenal mucous membrane. The clinical symptomatology is unspecific. As a rule, the diagnosis is made roentgenologically by a gastrointestinal passage. In the present case the diverticulum contained gallstones and could be represented by an intravenous cholangiography. This was conditioned by the position of the papilla in the diverticulum.

Adult↗
PubMed1979

[Laparoscopy in chronic gallbladder diseases].

The laparoscopy is the only riskless possibility of directly inspecting the gall-bladder, to ascertain the diagnosis of a chronic cholecystitis, further to consider the indications to internal or surgical measures. For the judgment the form of the gall-bladder, the behaviour of its wall with different colouring, oedema and furthermore its behaviour to neighbouring organs are of decisive evidence. In a disease of the bile-ducts the laparoscopy allows to prove or to exclude a participation of the liver. For the judgment of the activity of a disease of the gall-bladder should, however, always be used clinical and radiological findings, the microbiological investigation of the A-, B-, and C-bile and the testing of the reflex of the gall-bladder. This is necessary with regard to the therapy.

Cholecystitis↗
PubMed1979

[Dysphagia, diagnostic and therapeutic measures].

Dysphagia is a symptom of organically or functionally conditioned diseases of the oesophagus. In all circumstances it demands an immediate clarification of the etiology considering appropriate therapeutical measures and above all may be first reference to the carcinoma of the oesophagus.

Deglutition Disorders↗
PubMed1979

[Comparison of the value of ERCP, angiography and scintigraphy in pancreatic diseases].

The endoscopic retrograde cholangiopancreaticography, angiography and scintigraphy have a different value in the diagnostics of chronic pancreatitis and carcinoma of the pancreas. The scintigraphy of pancreas has the lowest value. It should not alone be the basis for making the diagnosis or planning the treatment. In chronic pancreatitis the endoscopic retrograde cholangiopancreaticography has precedence of angiography. The chronic pancreatitis may angiographically be recognised only in the late phase. However, the angiography can give valuable references to the planning of the operation. At a suspicion of the pancreas malignoma the endoscopic retrograde cholangiopancreaticography and the angiography have nearly the same value in the plan of diagnostics and they both should be performed.

Angiography↗
PubMed1975

[Permeation-increasing activity of puncture-fluids: 2. Chromatographic and immunoelectrophoretic studies].

A human pleural effusion was separated by gel chromatography, DEAE-chromatography, disk-electrophoresis and immunoelectrophoresis. The fractions obtained were tested for the permeation-increasing activity. IgA, IgM, IgG, transferrin and haptoglobin are not responsible for a permeation-increasing effect. Albumin, alpha1-antitrypsin, acid alpha1-glycoprotein and alpha2-macroglobulin appear only in permeation-active fractions. Of these proteins, however, no direct effect on the permeability of animal membranes is known. It is discussed, whether proteolytic enzymes, which may be bound to these proteins are responsible for the increase of permeation.

Adult↗
PubMed1975

[Permeability enhancement by exudates: 1. Demonstration of high-molecular substances in the animal model].

By means of ultracentrifugation, gel chromatography and polyacryl amide electrophoresis the peritoneal exudates of the rabbit, which increased the permeability of animal membranes, were separated. Among the highly molecular fractions two regions were found, which possessed an activity increasing permeation. Following the chromatographic separation of exudates of the rabbit, which did not reveal an activity increasing permeation, were also found two regions with an activity increasing permeation. The cuases for the activation during the separation process are discussed.

Animals↗
PubMed1975

[Assays of placenta-iso-enzyme of alkaline serum-phosphatase with disk-elektrophoresis (author's transl)].

Alkaline serum-phosphatase may be subdivided with disk-electrophoresis in 7% poly-acrylamid gel into the iso-enzymes: liver-cell, bone- and ileum-phosphatase. In the 7% gel liver-cell and placenta phosphatase move together. Separation of these two can be achieved by raising the gel-concentration to 10%. The method is suitable for quantitative densitometric evaluation.

Alkaline Phosphatase↗
PubMed1975

[Effect of ampholines on blood coagulation: 1. Activation of factor VIII (antihemophilic globulin A)].

Polyaminopolycarboxyl acids (Ampholine, LKB producers Sweden) caused the antihaemophilic globulin (factor VIII) to be activated. This increase of activity revealed a linear dependence on Ampholine concentrations. Only those Ampholine molecules of alkaline pH areas positively charged under test conditions turned out to be efficient. The positive charge, therefore, seems to be significant for an activation. The coagulation activities were measured by determining the partial thromboplastin time (PTT) and the factor VIII in an one-phase test.

Amino Acids↗