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S Lindenberg

Publications and source records attributed to S Lindenberg.

At least 55 records · Page 3Linked to original sources
PubMed1992

Effect of slow and ultra-rapid freezing on cell surface antigens of 8-cell mouse embryos.

The distribution of four cell surface antigens (SSEA-1, SSEA-3, SSEA-4, and I) present on mouse preimplantation embryos was examined on 8-cell stage embryos immediately after flushing from the reproductive tract and after slow or ultra-rapid freezing. Frozen-thawed and nonfrozen embryos were also examined after culture in vitro for 5, 24, or 48 h. Immediately after thawing, embryos showed a disruption in the polarity of cell surface antigens SSEA-3, SSEA-4, and I, but no differences were detected in fluorescence intensity or various other staining characteristics. No long-term changes in the distribution of cell surface components were detected. Implantation and embryonic development were similar for frozen and nonfrozen embryos transferred to the uterine horns of pseudopregnant recipients.

Animals↗
PubMed1992

Gamete intrauterine transfer.

Gamete intrauterine transfer has been performed at a very limited scale until now. The main target group of patients for such a treatment would be women with infertility due to the tubal factor, but other infertile patients could benefit also, as the procedures reduce incubation time in the laboratory and implies fertilization in vivo. Few studies have been reported from 1982 to 1990 on the transfer of oocytes and spermatozoa directly to the uterus, and only five clinical pregnancies have been achieved. Transfer of oocytes and spermatozoa inside a biodegradable capsule has only been performed in one study comprising 26 cycles with no ensuing pregnancies. A critical evaluation and a status is carried out.

Embryo Transfer↗
PubMed1991

Intrauterine capsules for incubation of gametes and subsequent release of embryos.

In order to obtain incubation in uteri of spermatozoa, oocytes, and embryos, for treatment of patients referred for in vitro fertilization, a capsule was produced which could contain the human gametes and allow human fertilization and embryo growth after intrauterine introduction. Agar was chosen for capsule material and a mold was constructed for the production of capsules. The material was tested in vitro using mouse embryos and human oocytes and sperm. Intrauterine resolution was tested on mice and by insertion on 11 women. Empty capsules were inserted into the uterine cavity in 15 cycles the day after the luteinizing hormone peak and followed by daily ultrasound examinations. The resolution time was adjusted by changing the wall thickness of the capsules. The final type was dissolved after 3 to 4 days. No complications were observed and capsules could be inserted on all occasions. The major problem was expelling of capsules, which occurred in seven cycles. The problem seemed to be solved by the administration of indomethacin at the day of insertion.

Adult↗
PubMed1991

Intrauterine fertilization capsules--a clinical trial.

Treatment of 26 women with tubal infertility was attempted using intrauterine capsules loaded with oocytes and spermatozoa. The stimulation protocol was as used for in vitro fertilization and embryo transfer and consisted of short-term use of Buserelin, human menopausal gonadotropin, and human chorionic gonadotropin. Oocytes were collected by ultrasonically guided transvaginal aspiration, and spermatozoa were prepared by swim-up technique. The gametes were placed in agar capsules 4 hr after oocyte collection, and the capsules were introduced to the uterine fundus using an insertion tube and piston from an intrauterine device. Six complete capsules and parts of two other capsules were expelled. None of the women became pregnant, compared with a pregnancy rate of 21% per aspiration following in vitro fertilization and embryo transfer during the same period.

Buserelin↗
PubMed1991

Experimental studies on the initial trophoblast endometrial interaction.

6.1. Attachment and penetration of the uterine epithelium in the human: The present studies describe to our knowledge the only studies of human in vitro attachment of blastocysts to uterine epithelial monolayers. From these studies, using other mammalian species as controls, it is concluded that initial attachment and penetration of the epithelium in the human might be of the intrusive type also seen in the Rhesus monkey (Lindenberg et al, 1986; 1989; Enders, 1972; 1976). This implicates an intensive and intimate cell-cell recognition and interaction already at the stage of epithelial attachment of the human embryo. 6.2. Oligosaccharide determinants in implantation: In the mouse we have identified an oligosaccharide Lacto-N-fuco-pentaose I, which inhibits blastocyst attachment to uterine epithelial cells in vitro. Monoclonal antibodies recognizing this epitope have identified LNF I-like determinants in the secretion and on the surface of the mouse endometrial epithelium during early pregnancy. LNF I-HSA/BSA-FITC conjugates have identified a receptor for LNF I on the surface of the blastocyst at the time of implantation. Furthermore, this study demonstrates a redistribution of these determinants prior to the day of implantation, supporting the hypothesis, that LNF I on the surface of the uterine lumen may contribute to the recognition and attachment even during the initiation of implantation. Component(s) carrying LNF-1 in the secretion might be regulatory molecules helping to secure the proper time for implantation in the mouse. 6.3: Future aspects. Whether the proposed mechanism of blastocyst-endometrium adhesion can be applied to species other than the mouse has still to be determined, but there are indications that this is worth testing.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

Monoclonal antibody 43-9F as a sensitive immunohistochemical marker of carcinoma in situ of human testis.

Invasive germ cell cancer can be prevented if the neoplasia is diagnosed at the stage of carcinoma in situ (CIS). In routine histologic examination CIS may be overlooked, particularly in prepubertal individuals. The detection of this early malignancy may be greatly facilitated by use of immunohistochemical staining. The authors investigated the sensitivity of an immunohistochemical staining procedure with monoclonal antibody 43-9F in detection of CIS. Testicular specimens from 19 adult and two prepubertal individuals with CIS were tested. Positive staining reaction on the surface of malignant germ cells was encountered in all 21 specimens with CIS. The epithelial cells of the excretory ducts between testis and epididymis, including rete testis and canaliculi efferentes, reacted also positively with 43-9F. No staining was observed in nonmalignant testicular cells including Sertoli cells or Leydig cells in any of these samples or in testicular biopsy specimens from 27 adult and 11 prepubertal subjects without evidence of testicular neoplasia. Monoclonal antibody 43-9F was also found to react with cells of all four tested invasive testicular germ cell tumors. Thus, the monoclonal antibody 43-9F is a sensitive immunohistochemical marker of CIS germ cells and may also be of potential value in detection of invasive testicular cancer.

Adult↗
PubMed1990

Identification of specific serum proteins synthesized de novo by monolayer cultures of glandular cells of gestational endometrium.

Monolayer cell cultures (n = 3) of glandular epithelium of gestational endometrium obtained from three apparently healthy women undergoing elective termination of pregnancy (7-9 weeks gestation) were established. De novo synthesis of eight serum proteins (albumin, alpha 1-antitrypsin, ceruloplasmin, beta-lipoprotein, alpha 2-macroglobulin, fibronectin and complement factors C3 and C4) was demonstrated by the incorporation of radiolabelled substrate ([35S]methionine) employing autoradiography (AR) in combination with crossed immunoelectrophoresis (XIE), referred to as ARXIE, and line immunoelectrophoresis (LIE), referred to as ARLIE. By contrast, there was no evidence for de novo synthesis of IgA, haptoglobin and orosomucoid. Our findings suggest that the gestational endometrium may contribute to the production of several proteins considered to be synthesized and secreted mainly by the liver and reticulo-endothelial system. The simple techniques used here to identify the de novo synthesis of human serum proteins could be applied to investigate protein synthesis by a wide range of tissues and cells.

Blood Proteins↗
PubMed1990

Ultrasonic evaluation of endometrial growth in women with normal cycles during spontaneous and stimulated cycles.

Ultrasound scanning of the endometrium was performed through the menstrual cycle in normal, ovulating women in 20 unstimulated cycles (A), 13 clomiphene citrate (CC)/human menopausal gonadotrophin (HMG)/human chorionic gonadotrophin (HCG)-stimulated cycles (B) and 20 CC/HMG/HCG-stimulated cycles with oocyte collection (C). A total of 294 endometrial scans were performed and the texture and thickness of the endometrium registered. All data were related to the day of the luteinizing hormone (LH) peak or to HCG administration (day 0). There was no difference between groups in the image of the endometrium, which consisted of thin echogenic lines from day -10 until day 0 (99%). Three thin echogenic lines were seen in the pre-ovulatory endometrium in all cycles. During the luteal phase, confluent, thick echogenic lines were observed in all but two cycles, where the pre-ovulatory pattern persisted. The endometrium reached a mean thickness at days -1 to +1 of 6.4 mm in group A and 6.1 mm in groups B and C together. The endometrium was thinner at days -7 to -5 and -4 to -2 in groups B and C together (2.7 mm and 4.2 mm) compared with group A (4.6 mm and 5.8 mm) despite increased serum levels of oestradiol. At days +2 to +4, the endometrium was thicker in groups B and C together (7.2 mm) compared with group A (5.3 mm).

Chorionic Gonadotropin↗
PubMed1990

De novo synthesis of placental protein-14 (PP14) and not PP12 by monolayer cultures of glandular epithelium of gestational endometrium.

Nine monolayer cell cultures of glandular epithelium from gestational endometrium were established from six apparently healthy women undergoing elective termination of pregnancy (7-11 weeks gestation). Radiolabel incorporation studies showed increasing incorporation of [35S]methionine into proteins present in supernatant and cytosol fractions over 48 h. The secreted proteins represented approximately 20% of the total incorporation of methionine into cytosolic proteins. De novo synthesis and secretion of placental protein-14 (PP14) and not PP12 was identified by a novel combination of line immunoelectrophoresis and autoradiography. All monolayer cultures demonstrated the presence of radiolabeled PP14, but not PP12, in the culture supernatants. These observations suggest that the glandular epithelial cells are the major site of synthesis and secretion of PP14 in human gestational endometrium.

Antibodies↗
PubMed1990

Hormonal control of a carbohydrate epitope involved in implantation in mice.

Ovariectomy and hormone replacement of mice were used to examine the hormonal control of expression on the uterine surface of a carbohydrate determinant (lacto-N-fucopentaose I, LNF I), involved in the initial interaction between the blastocyst and the endometrial epithelium at implantation. Pseudopregnant mice mated with sterile males were also used to elucidate the impact of embryonic signals on the expression of this antigen on the uterine surface. Two groups of fucosylated structures could be distinguished; one group was predominantly dependent on maternal oestrogen and progesterone, while the other group appeared to be less influenced by the hormonal milieu.

Animals↗
PubMed1989

A comparative study of attachment of human, bovine and mouse blastocysts to uterine epithelial monolayer.

The in-vitro attachment of human, bovine and murine blastocysts to monolayer cultures of uterine epithelium were studied by transmission electron microscopy. The human trophoblastic cells intrude between uterine epithelial cells forming a multilayer during attachment in vitro, thus resembling the intrusive type of penetration observed in vivo. The bovine trophoblastic outgrowth resembled an epitheliochorial attachment as the trophoblast formed an attachment plate on top of the endometrial cells without penetration. In the murine attachment study, the trophoblast cells immediately displaced the uterine cells and formed contact with the culture vessel.

Animals↗
PubMed1989

A case of the ring 20 syndrome.

A 4-year-old child with a ring 20 chromosome mosaicism, low grade developmental delay, and seizures is described.

Chromosome Aberrations↗
PubMed1988

Distribution of some Gal beta 1-3(4)GlcNAc related carbohydrate antigens on the mouse uterine epithelium in relation to the peri-implantational period.

Using monoclonal antibodies of defined carbohydrate specificity we have looked at the distribution of various Gal beta 1-3(4)GlcNAc related oligosaccharide determinants in the mouse uterus during the first 6 days of pregnancy. Frozen sections of uterus from B6D2F1, B6CBF1 or B6D2F1/BOM female mice were incubated with the monoclonal antibodies and then with a fluorescein isothiocyanate conjugate of goat anti-mouse IgM and viewed by epifluorescence illumination. None of the antibodies bound specifically to stroma cells but antibodies recognising difucosylated Gal beta 1-3(4)GlcNAc structures, the monofucosylated type II determinant (SSEA-1) and an H type I oligosaccharide bound to cells of the uterine luminal epithelium and glands and to the uterine secretions. Antibodies recognising the three different types of saccharide showed independent changes in staining intensity during early pregnancy. The antibody which recognises H type I structures (667/9E9) showed a change in distribution from binding to most cells of the uterine epithelium in the non-pregnant mouse and on day 3 of pregnancy to binding restricted to areas of epithelial cells interspersed with non-staining clumps of cells between days 4 and 5 of pregnancy.

Animals↗
PubMed1988

The milk oligosaccharide, lacto-N-fucopentaose I, inhibits attachment of mouse blastocysts on endometrial monolayers.

Seven oligosaccharides isolated from human milk were tested for their effect in an in-vitro model of mouse blastocyst adhesion and trophoblast outgrowth on endometrial epithelial monolayers. One compound, lacto-N-fucopentaose I (LNF I), produced a significant reduction in the percentage of attached and outgrown blastocysts after co-culture for 72 h (P less than 0.001). No significant effect of any other tested oligosaccharide was obtained.

Animals↗
PubMed1987

Are ultrasonic-guided follicular aspiration and flushing safe for the oocyte?

Oocyte collection was carried out in 53 patients by ultrasonically guided abdominal puncture under local or epidural anesthesia. Follicles were aspirated and flushed two to six times using a syringe with culture medium. A total of 196 oocytes was collected and 84 of those (42.9%) were found in the flushes. Mechanical damage was observed in 5.1% of the oocytes. Cleavage rates in mature oocytes (157) after 48 hr in culture were similar in the aspirate group (56.5%) and in the flush group (54.2%). Ten clinical pregnancies were obtained, corresponding to a pregnancy rate of 18.9%.

Female↗