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Biomedical subjects

S M Crispin

Publications and source records attributed to S M Crispin.

26 records · Page 2Linked to original sources
PubMed1988

Crystalline corneal dystrophy in the dog. Histochemical and ultrastructural study.

Crystalline stromal dystrophy a condition resembling human Schnyder's crystalline stromal dystrophy, is described in both the Cavalier King Charles Spaniel and Rough Collie breeds of dog. Both clinical cases and controls were normolipoproteinaemic; neither showed evidence of arcus lipoides corneae. Lipid histochemistry indicated the presence of cholesterol, cholesterol ester, and phospholipid in the superficial stroma. Free fatty acids were also detected in some samples, but triglycerides were not detected in any samples. Electron microscopy showed lamellated and nonlamellated vacuoles both within and outside abnormal fibroblasts. Acicular and rhomboidal clefts, some with a characteristic notch, were observed mainly in extracellular locations, although they were also associated with dying fibroblasts. The epithelium, basal lamina, Descemet's membrane, and endothelium were unaffected, apart from an irregularity of the basal lamina in some specimens.

Animals↗
PubMedDate not supplied

The effect of dietary changes on plasma lipids and lipoproteins of six Labrador retrievers.

Six healthy, spayed female Labrador Retrievers (aged 5.5 to 11 years), kept under controlled conditions of exercise and housing, were fed a diet of 15 per cent fat, 24 per cent carbohydrate and 33 per cent protein for two weeks before and between each of three test diets, of differing fat to carbohydrate ratios (A 13:44, B 20:33, C 25:26 fat:carbohydrate gm per cent dry weight) given in a predetermined order for four weeks each. Fasting plasma lipoproteins and total fatty acids were measured weekly. In the fourth week of each test period post-prandial plasma lipoproteins were measured. Diets B and C increased total (P < 0.001) and LDL cholesterol (P < 0.05) whilst diet A increased HDL triglyceride (P < 0.05). Eicosapentaenoic acid (C20:5) increased on diets B and C, and oleic acid (C18:1) on diet A. Post-prandial hypertriglyceridaemia occurred on all diets. Chylomicron clearance was slower on higher fat diets. Relatively small dietary changes produced significant alterations of plasma lipids, lipoproteins and chylomicron clearance.

Animals↗
PubMedDate not supplied

The influence of lifestyle and diet on the lipoprotein profile of border collies.

Plasma lipoprotein cholesterol and triglycerides were determined in two groups of Border Collies, one actively working and the other pets. Baseline concentrations of total, low density lipoprotein (LDL) and high density lipoprotein (HDL) cholesterol were higher, and HDL triglyceride concentrations lower in the pet dogs. Lifestyle of the dogs was assessed by questionnaire completed by the owners. Measurement of exercise was made by visual observation and using a Caltrac activity monitor. The working dogs were a homogenous group with respect to lifestyle and diet, but the pet dogs showed differences in lipoprotein profile relating to housing, dietary fat and exercise intensity. Two diets with different levels of dietary fat (13 and 20 per cent dry weight) were given for two months each. After two months on either of the diets the cholesterol concentration of the working dogs increased and HDL triglyceride concentration decreased, and there were no significant differences between the groups, but very low density lipoprotein (VLDL) triglyceride increased significantly in the less active pet dogs.

Analysis of Variance↗