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Biomedical subjects

S M Ivanova

Publications and source records attributed to S M Ivanova.

At least 37 records · Page 2Linked to original sources
PubMed1992

[The clinico-immunological subtypes of systemic lupus erythematosus].

Based on clinico-immunologic studies subtypes of systemic lupus erythematosus (SLE) were distinguished. The ANF-R++H-DNA-CH50 variant determines acute onset of SLE with renal injury in the form of diffuse glomerulonephritis. The ANF-Sp-RNP-RF mirrors the development of Raynaud's syndrome, Sjögren's syndrome, polymyositis, pneumosclerosis and myocarditis. The ANF-Sp-Ro-RF variant is associated with skin derangement in the form of discoid foci, anular, papulosquamous eruption, vitiligo, hyperpigmentation, and cerebrovasculitis. The Ro-anti-Po, La-anti-La system is related to the idea of SLE, seronegative in accordance with ANF, when rat liver sections are used as a substrate in immunofluorescence.

Antibodies↗
PubMed1990

[Antinuclear, anticentromere and anti-ScL-70 antibodies in rheumatic diseases].

Methods are described that are used for the titration of antinuclear, anticentromere, and anti-Scl-70 antibodies in systemic scleroderma, systemic lupus erythematosus, and rheumatoid arthritis: indirect immunofluorescence with various antigenic substrates (sections of fresh-frozen rat liver and Hep-2 cell culture), counter-current immunoelectrophoresis, isolation of Scl-70 antigen. Use of Hep-2 cells as a substrate for indirect immunofluorescence was found clinically and diagnostically more effective since it permitted the detection of anticentromere antibodies and anti-Scl-70. Nucleolar, mottled, homogeneous, marginal immunofluorescence types were observed when rat liver sections and Hep-2 cells were used for substrates. Anticentromere antibodies and anti-Scl-70 were isolated significantly more frequently in systemic lupus erythematosus or rheumatoid arthritis.

Antibodies, Antinuclear↗
PubMed1986

[Antinuclear antibodies in Sjögren's disease and syndrome].

A study was made of the presence of antinuclear antibodies to Ro, La, nDNA, RNP and Sm in 18 patients with Sjögren's disease (SD) and in 13 patients with Sjögren's syndrome (SS). Anti-Ro and/or anti-La were revealed in 12 SD patients (67%) and in 3 SS patients (23%, P less than 0.05) only. Anti-nDNA and anti-RNP were detected in 39 and 15% of the SS patients respectively and were undetectable in the SD patients. Anti-Ro and/or anti-La were detected in all SD patients, III degree activity, and associated with the presence of Raynaud's syndrome, recurring nonerosive arthritis and the absence of adequate therapy. Anti-Ro only were detected in 44% of the SD patients only and in none of the SS patients. Higher values of ESR, RF and CIC were revealed in the SD patients with anti-La and especially anti-Ro.

Adolescent↗
PubMed1986

[Antioxidants--stabilizers of the Ca2+ transport enzyme system in sarcoplasmic reticulum membranes in vivo].

It has been demonstrated that natural and synthetic antioxidants of different chemical structures (alpha-tocopherol, butylated hydroxytoluene, 2-ethyl-6-methyl-3-hydroxypyridine) were capable of stabilizing enzymatic Ca2+ transport in sarcoplasmic membranes of the heart and skeletal muscles in vivo. Chronic administration of water-soluble antioxidant 2-ethyl-6-methyl-3-hydroxypyridine to young and old rats resulted in the increased rate of Ca2+ transport into sarcoplasmic reticulum vesicles of the heart and skeletal muscle homogenates. Keeping rats on vitamin E-rich diets supplemented with synthetic antioxidant butylated hydroxytoluene led to stabilization of Ca2+-ATPase against thermal denaturation in sarcoplasmic reticular membranes.

Animals↗
PubMed1977

Some aspects of energy metabolism in human blood erythrocytes under hypokinesia and during space flights.

The present investigation presents results of studying energy metabolism in blood erythrocytes in cosmonauts who made the first and the second space flights aboard the orbital station Salyut-4 and in test subjects who experienced 49-d of hypokinesia at -4 degrees. The data obtained throughout the postflight investigations and during the hypokinesia studies appeared to be identical, showing a decrease in glycolytic activity whereas ATP content remained unchanged.

Adenosine Triphosphate↗
PubMedDate not supplied

[Early and late responses to oxidative stress in human dermal fibroblasts of healthy donors and rheumatoid arthritis patients. Relationship between the cell death rate and the genomic dosage of active ribosomal genes].

A study was made of the effect of the oxidizing agent potassium chromate (K2CrO4, PC) on cultured dermal fibroblasts of a healthy donor and three patients with rheumatoid arthritis (RA). Characteristics of the rRNA gene (RG) complex-RG copy number, active RG (ARG) dosage, and 18S rRNA content--were determined for each cell line. In cells of the healthy donor, oxidative stress caused by low doses of PC (2-4 microM, 1-4 h) induced an early response, including a 50-80% increase in total RNA and rRNA. An appreciable activation of the nucleolus was observed cytochemically, by silver staining and morphometry. The early response grew considerably lower with the increasing passage number and/or PC concentration. Exposure to 6-12 microM PC for 24 h led to a progressive cell death (late response). The existence and intensity of the early response correlated positively with the cell survival during further culturing. Cells of the RA patients displayed almost no early response even at early passages: total RNA did not increase, and rRNA increased by no more than 10%. Cell disruption (apoptosis) during further culturing was more intense than in the line originating from the healthy donor. The apoptosis intensity characterized by the increase in the content of DNA fragments in the culture medium and in the caspase 3 activity, was inversely proportional to the ARG dosage in the genome. The results provide the first quantitative characterization of the early and late responses of cells to PC-induced oxidative stress and suggest a role of the ARG dosage in cell survival in stress.

Arthritis, Rheumatoid↗
PubMedDate not supplied

[Effects of UV-radiation on metabolism and structural-functional status of the rat's erythrocyte membranes].

In-vitro analysis of venous blood taken from rats irradiated by 300 and 5,000 J/m2 of UV showed no effect on metabolism and, therefore, energy and recovery systems of erythrocytes. Concentrations of 2,3-diphosphoglycerate and reduced glutathione were increased after irradiation by 5,000 J/m2. UV-irradiation at 10,000 J/m2 decreased adenosine triphosphate and phospholipids in blood and impaired the functional stability of erythrocyte membranes. Recovery of the membrane structure in 24 hrs. after irradiation suggests extended photochemical processes in cells and is consistent with the literary data about indirect effects of plasma proteins on the red cell function.

Adaptation, Physiological↗
PubMedDate not supplied

[Peculiarities of free radical and antioxidant statute in patients with systemic lupus erythematosus and rheumatoid arthritis].

The state of lipid peroxidation and antioxidant defence was investigated in sera of 66 patients with rheumatoid arthritis (RA), 50 patients with systemic lupus erythematosus (SLE) and in 235 healthy donors using the method of chemiluminescence and EPR-spectroscopy. The results indicate involvement of free radical oxidation in pathogenesis of RA and SLE. Peroxide and antioxidant imbalance was more pronounced in RA than in SLE.

Adolescent↗
PubMedDate not supplied

[Octafluoropropane inhalation toxicity].

Wistar rats were used to study inhalation toxicity of octafluoropropane (OFP, freon-218) at the following concentration: 300 g/m3 (4-hrs), 30 g/m3 (0.5 to 4 hrs), 3 g/m3 (8 hrs), and 0.3 g/m3 (16 hrs). According to the histological analysis, OFP at the concentrations of 300 and 30 g/m3 had a politrophic toxic effect. Target organs were the lung, trachea, bronchus, heart, kidney, and the adrenaL There were dystrophic and necrobiotic lesions in the upper airways epithelium. Subacute and chronic vesicular bronchiolitis developed on days 7 and 14, respectively. Visceral organs and brain were found plethoric and the lung was found hemorrhagic. Similar lesions were seen in the trachea, bronchus, lung, liver, spleen, kidney, adrenal, heart, and the brain. Lipid redistribution was observed in the adrenal cortex and vascular reactions of renal tissue with juxtamedullar blood shunting. Toxicity of small OFP concentrations (3 and 0.3 g/m3) was distinguished by an extended aftereffect, these concentrations did not cause visible pathomorphologic changes but gave rise to an extended pathologic process detectable by biochemistry. In all concentrations, OFP impacted erythrocyte metabolism changing the lipid composition of cell membrane and activating membrane-bound adenosinetriphos-phatases. The activities of hepatocyte and myocardiac cytoplasmatic enzymes were altered in blood plasma. Increased malonic dialdehyde in blood plasma and decreased cell antioxidant GSH in erythrocytes suggested exaggerated lipid peroxidation. These data point to the necessity of revising the existing limits for OFP concentrations in air of populated areas, working areas, and closed human environments.

Administration, Inhalation↗