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Biomedical subjects

S M Shane

Publications and source records attributed to S M Shane.

At least 37 records · Page 2Linked to original sources
PubMed1986

The viability of Campylobacter jejuni on refrigerated chicken drumsticks.

Radappertized chicken drumsticks were experimentally contaminated with suspensions of Campylobacter jejuni in two trials. Qualitative analysis on drumsticks with an initial level of contamination of 4.8 X 10(3) CFU/cm2 showed that viability was retained for at least 10 days of storage at either 9 degrees or -12 degrees C. In a second quantitative trial, the level of contamination declined from 9.9 X 10(2) CFU/cm2 to 4.5 X 10(1) CFU/cm2 after 7 days at -20 degrees C. Thereafter, C. jejuni persisted at levels ranging from 1.8 X 10(1) to 0.2 X 10(1) CFU/cm2 through the 26th week of storage. Drumsticks held at 4 degrees C showed a significant decline in count from 9.9 X 10(2) CFU/cm2 to 1.8 X 10(2) CFU/cm2 on day 7. It is concluded that the viability of C. jejuni on chicken parts is maintained under both refrigerated and freezing conditions which approximate commercial storage. This is of significance to the meat industry and consumers.

Animals↗
PubMed1986

Campylobacter jejuni contamination of eggs.

Contamination of commercial table eggs with a fecal suspension containing 4.4 X 10(6) CFU/g Campylobacter jejuni resulted in shell penetration in 3/70 eggs and recovery of the organism from homogenized egg contents in 1/70 eggs. Viability of C. jejuni on the shell surface was retained for only 16 hours, attributed to desiccation of the fecal suspension. A field survey of three commercial laying farms and their associated egg-packing plants showed that hens demonstrated to be fecal shedders of C. jejuni (12% to 62% incidence) did not produce infected eggs. The organism could not be detected in the environment of the packing plant, including grading machinery and effluent.

Animals↗
PubMed1986

A case of Dipylidium caninum in Baton Rouge, Louisiana.

The relevant literature concerning human dipylidiasis has been reviewed with specific reference to the association of children and their pet dog and cats. A specific case is recounted which is considered to be typical of the condition as encountered by pediatricians and general practitioners.

Animals↗
PubMed1985

The occurrence and significance of Campylobacter jejuni in man and animals.

Campylobacter jejuni, which is now recognized as a discrete species, is a gram negative, microaerophilic, thermophilic, nalidixic acid sensitive, hippurate positive pathogen requiring special selective media for propogation. The organism is widely distributed in avian species, experimental and companion animals and in humans. Mammalian campylobacteriosis is characterized by an enterocolitis of variable severity. The prevalence of the condition is relatively high in young individuals, in underdeveloped countries and in subjects with diarrhea. Food animals, especially poultry, are reservoirs of the organism and infection occurs following consumption of untreated surface water, unpasteurized milk, incompletely cooked meat or other contaminated food products. Close contact with infected immature companion animals is a significant cause of campylobacteriosis in children and direct intrafamilial transmission and occupational infection have been documented. Campylobacteriosis attributable to C. jejuni is a condition of emerging significance which arises principally from deficiencies in hygiene inherent in the environment and in the food chain which extends from domestic animals to the consumer.

Animals↗
PubMed1985

Etiology and pathogenesis of necrotic enteritis.

Sporulated oocysts of Eimeria acervulina were administered orally to cage-housed broilers at a dose of 3.5 X 10(5) resulted in mild subclinical coccidiosis. Clostridium perfringens incorporated in feed at a level of 2.5 X 10(8) organisms/g. produced lesions characteristic of necrotic enteritis. Mortality of 8% (7/80) occurred in birds fed a ration inoculated with Cl. perfringens alone. Mortality of 35% (28/80) was observed in birds which received an oral dose of E. acervulina and which were fed simultaneously with a ration containing Cl. perfringens. Birds which were fed an inoculated ration two days after an oral dose of E. acervulina showed 41% (33/80) mortality. Birds which received an inoculated ration for two days before administration of an oral dose of E. acervulina demonstrated 18% mortality (15/80). Birds which were fed an inoculated ration four days after an oral dose of E. acervulina showed 10% mortality. Infection with E. acervulina reduced the pH of intestinal contents with a simultaneous depression in serum protein. A 39% increase in intestinal passage time from 178 to 248 minutes occurred on the fifth day after infection with E. acervulina. These experiments suggest that necrotic enteritis, attributed to proliferation of a toxigenic strain of Cl. perfringens, followed intestinal stasis and minimal lesions induced by mild intestinal coccidiosis.

Animals↗
PubMed1985

Prevalence of Campylobacter jejuni in felidae in Baton Rouge, Louisiana.

A prevalence study revealed Campylobacter jejuni in rectal swabs of 1% of a sample of 430 domestic cats and from one species of 15 zoo felines which were examined. There was no correlation between age, presence of diarrhea, source of sample (animal shelter, veterinary clinic, domestic pet) and the isolation of C. jejuni. A controlled exposure trial confirmed that fecal shedders can transmit infection to susceptible contacts which subsequently demonstrate transient diarrhea. Infected cats show a variable pattern of excretion of the organism which could result in failure to determine C. jejuni status based on a single examination.

Animals↗
PubMed1982

Conscious sedation and behavioral modification with local standby anesthesia for ophthalmologic surgery.

Local and retrobulbar block anesthesia in combination with a brief span of amnesia - not general anesthesia -- produced by minute doses of four intravenous sedative drugs, has made it possible to perform ophthalmologic surgery without the memory of the pain of the retrobulbar block, followed by an ensuing quiescence augmented by behavioral modification for the duration of the operation. Patients are awake, rational, cooperative, calm and relaxed, and because of behavioral modification, are highly motivated to guarantee not only their own survival, but to feel a part of the team effort to preserve the integrity of their eye.

Amnesia↗
PubMed1981

Sequential mitogen stimulation of peripheral blood lymphocytes from chickens inoculated with infectious bursal disease virus.

Chickens were inoculated wih the pathogenic Edgar strain of infectious bursal disease virus at 1 week, 2 weeks, or 1 day of age. In the 3 experiments, phytohemagglutinin stimulation of peripheral blood lymphocytes was significantly decreased on day 3 or 4 after inoculation. Subsequently, on days 7 through 21, stimulations were similar between lymphocytes from inoculated birds and those from control birds. Pokeweed mitogen stimulation was affected minimally in virus-inoculated chickens. In each experiment, on day 7, the spontaneous [3H]thymidine uptake was greater in nonstimulated lymphocyte cultures from inoculated chickens than in such cultures from control chickens. In an additional experiment, chickens 1 week of age were exposed to a pathogenic vaccinal virus given in their water. The vaccinal virus exposure resulted in significant decrease of phytohemagglutinin stimulation of lymphocytes on days 3 and 7 of the experiment. A significant decrease in pokeweed mitogen stimulation was observed on day 10 after inoculation.

Animals↗