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Biomedical subjects

S M Shane

Publications and source records attributed to S M Shane.

64 records · Page 4Linked to original sources

The occurrence of Clostridium perfringens in the intestine of chicks.

Commercial broiler chicks brooded either on wire or on used or new litter demonstrated a 75% (62/75) incidence of recovery of "perfringens-like" colonies from the intestine during a 5-week period. Eleven Clostridium spp. were identified from among these "perfringens-like" organisms, which were cultured on SPS selective agar medium. Clostridium perfringens was positively identified only infrequently (five isolates) from among the "perfringens-like" colonies. In contrast, "perfringens-like" colonies were not recovered from the intestinal contents of specific-pathogen-free chicks reared in an isolation unit. However, C. perfringens was isolated from the yolk sac of one embryonated egg and from the intestine of a single 7-day-old chick, indicating the possibility of vertical transmission of this potential pathogen.

Animal Feed↗

The occurrence of Aeromonas hydrophila in avian diagnostic submissions.

Twenty isolations of Aeromonas hydrophila were made from 15 species of free-living, commercial, and companion birds submitted for routine diagnostic postmortem examination. Seventy percent of the isolations were made from November through March during the 25-month survey period.

Aeromonas↗

Tuberculosis in commercial emus (Dromaius novaehollandiae).

Extensive granuloma formation typical of tuberculosis was observed in a mature female emu. The diagnosis was confirmed by demonstration of acid-fast bacilli in lesions and culture of a Mycobacterium with growth characteristics resembling M. avium from liver tissue. Individual emus on the affected farm and an epidemiologically related unit gave a positive skin reaction to intradermal M. avium tuberculin. The implication of tuberculosis in commercial emus is noted in relation to the growth of the industry in North America and to management and commercial practices that encourage dissemination of infection within the species and to other exotic and domestic animals.

Animals↗

Comparison of procedures to detect Chlamydia psittaci antibodies in cockatiels (Nymphicus hollandicus).

Diagnostic serologic procedures for psittacine chlamydiosis were evaluated using experimentally inoculated cockatiels (Nymphicus hollandicus). Chlamydia psittaci was recovered from subjects on day 16 postinoculation. Seroconversion was confirmed by the direct complement fixation (DCF) test. The blocking enzyme-linked immunosorbent assay was demonstrated to be the most sensitive of three serodiagnostic procedures evaluated from inoculation until day 24 postinfection, with 92% sensitivity and 73% specificity. The DCF test was progressively more sensitive over the duration of the experiment, attaining 100% sensitivity and specificity on the day 24 postinfection. This study confirms the ability of the DCF procedure to adequately detect C. psittaci antibodies. Limitations relating to reproducibility of serologic results under clinical conditions found in this study indicate that a further refinement of tests is required to consistently detect and quantify antibodies.

Analysis of Variance↗