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S M Spaethe

Publications and source records attributed to S M Spaethe.

42 records · Page 3Linked to original sources
PubMed1981

Schultz-Dale reaction in mouse trachea.

A method was developed to induce contraction of immunologically sensitized mouse trachea by antigen (Schultz-Dale reaction). The response was mediated by immunoglobulin (Ig) E antibody directed against either the hapten DNP, the hapten carrier conjugate DNP-keyhole limpet hemocyanin (KLH), or the unmodified carrier KLH. Tracheal contractions were elicited by DNP-KLH, KLH, or DNP-bovine serum albumin (BSA) but not by DNP or BSA alone. This procedure represents a useful index of in vitro anaphylaxis in mouse airway smooth muscle.

Anaphylaxis↗
PubMed1979

Computerization of a bioassay: quantitation of slow reacting substance of anaphylaxis (SRS-A).

The bioassay for SRS-A using isolated guinea-pig ileum bathed in atropine and pyrilamine has been computerized resulting in a more accurate collection and calculation of data. Areas under the polygraph tracings are calculated by a computer interfaced with the recording polygraph. After comparison of ileal responses obtained with known amounts of SRS-A standard, the computer determined the relative amounts of SRS-A in unknown samples. The program provided for a correction to adjust for changes in tissue responsiveness. Using this bioassay, we showed that ovalbumin-induced release of SRS-A from sensitized guinea-pig lung was parallel to the release of histamine measured fluorometrically. Ethanol inhibited release of these mediators of anaphylaxis in a similar fashion. Conversely, isoproterenol reduced ovalbumin-induced release of SRS-A to a greater extent than the release of histamine. The versatility of this technique should enable other bioassays to be improved in addition to greatly facilitating the determination of various pharmacologic analyses.

Animals↗
PubMedDate not supplied

Vasodilation and aging evaluated in the isolated perfused rat mesenteric vascular bed: preliminary observations on the vascular pharmacology of dobutamine.

The influence of animal age of drug-induced vasodilation was investigated in the perfused rat mesentery constricted with norepinephrine. Responses to isoproterenol, a beta-receptor stimulant, decreased with increasing age. Also, there was a modest decline in the relaxation produced by papaverine. In contrast, dilation of the rat mesentery by acetylcholine, histamine, or nitroglycerin either did not change with age or the responses became somewhat larger. Dobutamine, a myocardial beta-receptor stimulant, produced a marked relaxation of the mesentery. A comparison of the action of isoproterenol and dobutamine revealed that isoproterenol stimulated vascular beta-receptors, whereas dobutamine relaxed the mesentery by antagonizing the tone produced by norepinephrine. The dilation resulting from the alpha-receptor-blocking action of dobutamine was unrelated to animal age.

Adrenergic alpha-Agonists↗