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S MITSUHASHI

Publications and source records attributed to S MITSUHASHI.

At least 37 records · Page 2Linked to original sources
PubMed1963

DRUG RESISTANCE OF ENTERIC BACTERIA. II. TRANSDUCTION OF TRANSMISSIBLE DRUG-RESISTANCE (R) FACTORS WITH PHAGE EPSILON.

Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resistance to tetracycline (TC), chloramphenicol, streptomycin, and sulfonamide by cell-to-cell contact, were found to be transduced in the system of Salmonella E group with phage epsilon (epsilon(15) and epsilon(34)). The R(+) transductants of S. newington (S-84) and S. chittagong (S-224) were all found to be unable to transfer their R factors by conjugation, and their R factors were not eliminated by treatment with acridine dyes so far as tested. The R factors containing TC resistance were consistently segregated when transduced. At low multiplicities of infection, the R(+) transductants with epsilon(15) were all nonlysogenic and unable to produce normal epsilon(15) phage particles; among the R(+) transductants with epsilon(34), 34% were lysogenic and 66% were sensitive to epsilon(34).

Acridines↗
PubMed1962

Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine.

Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier studies it was shown that mice hyperimmunized with live vaccine of Salmonella enteritidis resisted intravenous challenge with 1,000 MLD of virulent strain 116-54 of S. enteritidis. Survivors of this challenge completely resisted additional intravenous challenge with 10,000 MLD of the same organism. Mononuclear phagocytes obtained from the abdominal cavity of mice immunized with live vaccine of S. enteritidis inhibited intracellular multiplication of virulent strain 116-54, regardless of the presence of antibody in the cell-culture medium. In the present study, mononuclear phagocytes were obtained in a nearly pure state from liver or subcutaneous tissue of mice and were maintained in vitro in good condition. These cells also resisted cellular degeneration caused by intracellular existence of virulent strain 116-54, regardless of the presence of antibody in the cell-culture medium. In contrast, cells obtained from normal mice or mice immunized with dead vaccine were subject to degeneration.

Animals↗
PubMed1962

Combination of two types of transmissible drug-resistance factors in a host bacterium.

Mitsuhashi, Susumu (Gunma University, Maebashi, Japan), Kenji Harada, Hajime Hashimoto, Mitsuo Kameda, and Mitsue Suzuki. Combination of two types of transmissible drug-resistance factors in a host bacterium. J. Bacteriol. 84:9-16. 1962.-When two types of R factor, R(TC) and R (CM.SM.SA), or R(TC) and R (CM), were brought together in a host bacterium by superinfection with both factors, loss of either one or both factors was found. In the imperfectly stable existence of both factors in a host bacterium, both factors were transmitted separately by conjugation. As the result of interaction between the two types of R factor present in a host bacterium, recombinant factors were formed, R(25) (TC.CM.SM.SA) and R(31) (CM.TC). The recombinant factors were able to transfer their resistance by conjugation. They were also transduced as one unit into Escherichia coli K12 by Plke phage in the same fashion as the original R(11) (TC.CM.SM.SA) and R(14) (CM.TC) factors independently isolated from dysenteric patients.

Bacteria↗