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Biomedical subjects

S Martinov

Publications and source records attributed to S Martinov.

24 records · Page 2Linked to original sources
PubMed1981

[Inactivated vaccines against rhinopneumonitis in horses].

Attempts were made to produce inactivated vaccines against horse Herpes virus 1, using various inactivating agents and adjuvants, Best results were obtained with vaccine No 3 (glutaraldehide inactivator and "CTC" adjuvant). Used were two strains of the virus (St. Karaja and Varna). isolated in this country in cell cultures of a sucking pig kidney. Vaccine No 3 showed good immunogenic properties. Its application resulted in the full cease of abortions and respiratory diseases on the base of infection with the horse Herpes virus 1. The vaccination protects newborn colts from rhinopneumonitis if reimmunization of mares is carried out in the 6th-7th month of pregnancy.

Animals↗
PubMed1981

[Method of obtaining concentrated and purified chlamydial suspensions].

A method was worked out to obtain concentrated and purified chlamydial suspensions. Used were differential centrifugation and super-centrifugation, gel filtration on sepharose, and ultracentrifugation on saccharose substrate. The new method was successfully employed in electron microscopic, immunologic, and immunofluorescence investigations. As a result of its use in electron microscopy studies were carried out on the morphology of the purified chlamydial particles in ultra thin sections and negatively contrasting preparations. Immunologic studies led to the production of high-titer hyperimmune sera from guinea pigs. Such native sera were successfully used to obtain high-titer fluorescent sera.

Animals↗
PubMed1981

[Serological studies in bovine Q fever].

A serologic study was carried out by means of the complement-fixation test (CFT) and the immunofluorescence titration method (ITM) of a total of 1250 blood samples from 13 farms, having records of abortions, retention of placentae, and endometritis. The comparison of CFT and ITM results showed that 44 (52.3 per cent) of the positive sera were altogether positive by the two methods, 24 (28.6 per cent) sera yielded positive reactions by CFT and negative ones by ITM, and 16 (19 per cent) sera proved positive by ITM and negative by CFT. In two cows that were experimentally infected with rickettsiae the complement-fixing antibodies appeared between the 8 h and 15th day, and persisted up to the 160th day, their titers reaching peak values of up to 1:160. Recorded was the dynamics of the complement-fixing antibodies in spontaneously infected cows that miscarried. In this case too the titers were not high--1:10 to 1:40. They persisted in the course of six months.

Animals↗
PubMed1980

[Morphogenesis of Chlamydia].

A morphological investigation was carried out on more than 12 chlamydia strains of avian and mammalian origin, multiplied in chick embryos. A number of investigations were carried out under an electronic microscope, including 674 samples of concentrated and purified chlamydia as well as yolk sacs in ultrathin sections and in negatively contrasted preparations. In the cytoplasm of the cells we proved the presence of a chlamydial matrix, an autonomous synthesis of chlamydial bodies, the morphology and polymorphism of the chlamydiae, as well as the degenerative changes in the endodermal and endothelial cells in the yolk sacs of the chick embryos. Conclusions were drawn out that in the development of chlamydia in the chick embryos no intertransformation of chlamydial bodies and no binary divisions are observed, i.e. there are no proofs of the so-called cycle of development. The development of chlamydia in chick embryos takes place in the form of specific chlamydia morphogenesis which is characterized by an autonomous synthesis and morphogenesis of chlamydia bodies on the basis of a multivacuolated matrix.

Animals↗
PubMed1979

[Complement fixation reaction studies in rhinopneumonitis of horses].

It was established that the complement binding reaction (CBR) is a suitable and very fast method for horse rhino-pneumonitis diagnostics. Cell cultural virus produced in cell cultures of pig kidneys was used as antigen. The antigen lots tested have no anticomplementary properties. Highest complement binding activity was evident in the non-diluted antigen, which discovered specific antibodies in immune serums. The CBR specificity was tested by the aid of homologous and heterologous serums and antigens. The titers of complement binding antibodies in the serums of 255 horses recovered from the disease varied from 1:10 to 1:320, and in some cases even to 1:640. The blood samples investigated were from studs having rhinopneumonitis proven by clinical observations, by virus neutralizing reaction and by virus isolation. In almost all animals under study neutralizing and complement binding antibodies were established simultaneously.

Animals↗