Biomedical subjects

S Mboup

Publications and source records attributed to S Mboup.

93 records · Page 6Linked to original sources

PubMedDate not supplied

Relation between HIV-2 proviral load and CD4+ lymphocyte count differs in monotypic and dual HIV infections.

OBJECTIVE: To explore and compare the relations between proviral DNA load and CD4+ lymphocyte counts in both HIV-2 monotypic and HIV dual infection. STUDY DESIGN/METHODS: In Dakar, Senegal, where the HIV-1 and HIV-2 epidemics overlap, serum and peripheral blood mononuclear cell (PBMC) DNA samples were collected from registered female sex workers and hospitalized patients. Sera were evaluated for reactivity to antigens of HIV-1 and HIV-2 by immunoblot; dual reactivity was confirmed with recombinant envelope peptides for HIV-1 and HIV-2. These samples were then subjected to HIV-1 and HIV-2 proviral DNA polymerase chain reaction (PCR). To evaluate the HIV-2 cellular proviral DNA loads, a quantitative competitive PCR (QC-PCR) was developed using nested primers to amplify the gag region of HIV-2. This assay used an internal competitor generated by inserting 25 bp in the first-round PCR target sequence. T-lymphocyte subset counts were estimated by flow cytometry for both HIV-2 monotypic and dually infected persons. RESULTS: 35 HIV-2-infected and 33 dually seroreactive samples were evaluated in this study. The CD4+ lymphocyte counts were similar in both groups, with mean values of 449 +/- 390 cells/mm3 for the HIV-2 monotypic infected persons and 476 +/- 308 cells/mm3 among the dually infected persons. However, the median proviral loads differed significantly, with those in the HIV-2 group ranging from 63.2 to 669.8 copies/10(5) CD4+ cells and demonstrating an inverse correlation with CD4+ lymphocyte count. The HIV dually infected persons showed less variation in viral load, ranging from 9.9 to 43.3 copies/10(5) CD4+ cells. Among the HIV dually infected persons, low HIV-2 proviral load was correlated with low CD4+ lymphocyte counts. CONCLUSIONS: The HIV-2 proviral loads in HIV dually infected persons were significantly lower than those in HIV-2 monotypically infected individuals (P < .0001), despite comparable CD4+ lymphocyte counts. These results suggest that different HIV-2 proviral dynamics prevail in HIV dual infection.

CD4 Lymphocyte Count↗

PubMedDate not supplied

[Place of Staphylococcus aureus in the bacterial isolations of the University Hospital Center of Dakar. Preliminary results of serotyping and phage typing].

Out of 18 837 strains isolated between 1972 and 1980, by the laboratory of bacteriology of the Dakar Teaching Hospital, 2 925 strains of Staphylococcus aureus have been identified. This germ was the cause of 14,4 p. 100 of all diagnosed septicemia, ranging second in frequency after Salmonella. It was the most frequent germ in pura (54,8 p. 100 of strains) and rated 29,2 p. 100 of the strains isolated from pleural fluids. It was less frequent in bacterial meningitidis (2,5 p. 100) and in infections of the urinary tract (3,6 p. 100). About one hundred coagulase + strains were studied further. A clumping factor was found in 98 p. 100 of the strains (Staphyslide test). In addition, serotyping and lysotyping were carried out: --80,8 p. 100 of strains were lysotyped --25,6 p. 100 of them belonging to type I --7,7 p. 100 to type II --10,3 p. 100 to type III --33,3 p. 100 belonging to two or three types --11,5 p. 100 to I and II --16,7 p. 100 to I and III --5,1 p. 100 to I and II and III Some strains gave positive responses with 94-96 and 187 phages.

Bacteriophage Typing↗

PubMedDate not supplied

[Evaluation of the risks of sexually transmitted diseases and HIV infection in a rural region of Senegal in 1991].

This study was performed in the Sereer region 150 km to the east of Dakar, the capital of Senegal. The population of the region is characterised by large seasonal migration, a high divorce rate (41% of marriages end in divorce) and frequent polygamy (1.8 married women per married man). We organised the medical centres in the region to monitor actively the epidemiology of HIV infection. Three populations were targeted: pregnant women presenting for their first prenatal consultation; patients presenting with STD; and people with chronic (more than three weeks) cough. The patients consulting for STD were recruited two ways: those presenting spontaneously and those identified during home interviews by the team performing a parallel sociological study of behaviour. Overall, the prevalence of HIV seropositivity was 0.2% of the women and 1.3% of the men (the difference is not significant). The seropositive individuals identified were 2 pregnant women and 5 patients (3 of 409 women and 2 of 84 men) with STD. There was no significant difference between the sex, age, marital status, or type of recruitment (spontaneous or identified by the sociological survey) of the HIV seropositive and seronegative individuals. The prevalence of treponema antibodies was 1.8% among pregnant women, 2% among STD patients and 2.4% among patients with chronic cough. There was no significant difference according to age, sex, marital status or motivation for consultation. More than half the patients consulting for STD presented biomedical disease. The majority of the STD patients were women: 70% of those spontaneous consulting; 90% of those identified by the survey; and 92% of those with biomedical disease were women.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗