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S Mboup

Publications and source records attributed to S Mboup.

At least 91 records · Page 5Linked to original sources
PubMed1989

[Electrophoretypes of the genome of rotaviruses isolated from Senegalese children].

Polyacrylamide gel electrophoresis allowing study of rotavirus genome was applied to 213 faecal specimens from children with acute gastroenteritis living in Dakar area, Senegal. We were able to define an electrophoretype in 41 cases (19.2%). Five different RNA electrophoretypes were shown. The electrophoretype 4 was the most frequent (41.4%). "Long" and "short" electrophoretic migration patterns were found in 36 and 5 cases, respectively. We found 34.1% of electrophoretypes in children aged from 6 to 11 months. The electrophoretypes 1, 3, 4 and 5 have co-circulated throughout the study period (March 1985 to August 1986). No mixed infections were found. Large surveys of this type would allow to evaluate the general situation in Senegal in urban areas as well as in rural areas.

Child, Preschool↗
PubMed1989

Hepatitis B virus infection in lepromatous and tuberculoid patients from Senegal.

Hepatitis B Virus (HBV) seric markers (HBs Ag, anti-HBs, and HBe Ag) were studied in 987 Senegalese leprosy patients (lepromatous: LL; tuberculoid: TT) in comparison with 6187 healthy adults (controls). Two populations of leprosy patients from ILAD (Institut de Léprologie Appliquée de Dakar) were studied: The First study (i.e.: study I) between 1973 and 1977 included 553 patients (329 LL; 224 TT). The Second study (i.e.: study II) between 1982 and 1986 included 434 patients (236 LL; 198 TT). HBV serological markers were tested by various techniques. By RIA, they were present in 98% and 96.5% in the studies I and II respectively. Each marker was studied and compared to the control population. HBs Ag detected by RIA was present in 25.5% (study I) and 23.0% (study II) when comparing to 15.2% in the control group. This marker was correlated with leprosy forms (LL and TT), age, sex, ethnic group.

Female↗
PubMed1986

Persistence of circulating complexes between HBsAg and immunoglobulin M in sera of hepatitis B surface antigen positive patients suffering from liver cirrhosis or primary liver cancer.

Complexes between hepatitis B surface antigen (HBsAg) and immunoglobulin M (IgM) have been detected in acute type B hepatitis. Sequential serum testing for the presence of these complexes has been shown to be the best method for predicting disease chronicity. The presence of HBsAg/IgM complexes was investigated using an enzyme-linked immunosorbent assay with selected sera from Senegal. The three population groups studied were composed of 405 Senegalese soldiers as well as 84 liver cirrhosis and 169 primary liver cancer patients. Only one of the 122 HBsAg negative sera tested was found to be positive for HBsAg/IgM complexes. Complexes were detected 13.9% of the HBsAg positive soldiers, in 40% of the HBsAg positive liver cirrhosis patients, and in 50% of the HBsAg positive primary liver cancer patients. HBsAg/IgM complexes were also detected in 53.6% of the hepatitis B e antigen (HBe) positive soldiers, compared to 75 and 76% for the HBeAg positive liver cirrhosis and primary liver cancer patients, respectively. In anti-HBe positive sera, an increased proportion of HBsAg/IgM complexes was observed during the sequence chronic hepatitis (5%)-cirrhosis (29%)-primary liver cancer (42%). On the other hand, it has been reported that in the sequence of events leading from chronic hepatitis to primary liver cancer, there is an increase in anti-HBeAg prevalence and in alpha-fetoprotein levels. In this study, only alpha-fetoprotein levels were found to increase. Values higher than 15 IU/ml were observed in 4.3, 27.3, and 86.4% of the HBsAg positive individuals from the three groups. No significant variation was observed in the anti-HBe prevalence between the population group (64-75%).

Antigen-Antibody Complex↗
PubMed1985

[Antimicrobial activity of some medicinal species from the Dakar markets].

After drawing up an inventory of Dakar markets popular medicinals plants, antimicrobial activity of 43 species have been screened. With the agar diffusion method, crude extracts of 24 species show an activity against Sarcina lutea or Escherichia coli. The antimicrobial spectrum of these extracts against 11 germs has been evaluated with the agar dilution method. 13 species show M.I.C. lower than 10 mg/ml against some germs. This preliminary study select some plants of potential interest for development of new antibacterial.

Anti-Bacterial Agents↗
PubMed1983

[Latex agglutination test and counterimmunoelectrophoresis in the diagnosis of Haemophilus influenzae meningitis].

Authors have searched for Haemophilus influenzae by culture and by detection of capsular antigens in CSF of 1.700 bacterial meningitis in Dakar. Culture has been positive in 71.9%, counter-immunoelectrophoresis in 94.9% and latex (slidex meningitis kit) in 91.2% of cases. Diagnosis results are improved of 39% comparatively with bacteriologic culture. False positive results caused by antigenic cross-reactions have been observed only in 1.3% of others bacterial meningitis. Latex-test is allowing a diagnosis in three minutes and a quantitative determination of antigens (useful for pronostic).

Counterimmunoelectrophoresis↗
PubMed1980

[Antibacterial effect of borreverine, an alkaloid isolated from Borreria verticillata (Rubiaceae)].

Borreria verticillata, a very common tropical plant, is used in traditional pharmacopeia to recover cutaneous infections. The Borreverine alkaloid extracted from this plant had an antimicrobial action in vitro. The minimal inhibitory concentration is lower than 50 micrograms/ml for Gram positive cocci, (specially Staphylococcus aureus) and than 6 micrograms/ml for Vibrio cholerae and upper than 200 micrograms/ml for several Gram negative rod-bacteria (Enterobacteria and Pseudomonas). These preliminary results underline the interest in the research about the antimicrobial agents from plant origin, in particular concerning naturally or chemically modified alkaloids.

Alkaloids↗
PubMedDate not supplied

Role of the CCR5 delta 32 allele in resistance to HIV-1 infection in west Africa.

OBJECTIVE: To determine the frequency of the mutant CCR5 delta 32 allele in high-risk HIV-seronegative Africans as compared with the general African population, and to assess its in vitro protective efficacy against HIV-1 infection. STUDY DESIGN: In the homozygous form, the CCR5 delta 32 allele confers resistance to macrophage-tropic (M-tropic) strains of HIV-1. Assuming that genetic characteristics favoring HIV resistance would prevail in a high-risk HIV-seronegative population, we examined the CCR5 genotypes of female commercial sex workers (CSWs) from Dakar, Senegal, who have remained uninfected for an elongated period. METHODS: The CCR5 genetic profile of study participants was determined by polymerase chain reaction (PCR) amplification of genomic DNA followed by sequencing. Peripheral blood mononuclear cells (PBMCs) were infected with different strains of HIV-1 and monitored by p24 enzyme-linked immunosorbent assay (ELISA). RESULTS: We confirmed the presence of two CCR5wt/delta 32 genotypes among 139 individuals (1.44%). PBMCs from these 2 heterozygous individuals were also found to be less susceptible to in vitro infection by an M-tropic HIV-1 primary isolate. CONCLUSIONS: Evidence was found of an increased prevalence of the CCR5wt/delta 32 genotype in a high-risk HIV-seronegative cohort in West Africa. Furthermore, reduced susceptibility to HIV-1 infection among heterozygous individuals supports a role for 32-bp CCR5 deletion in HIV-1 resistance.

Acquired Immunodeficiency Syndrome↗