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S Misra

Publications and source records attributed to S Misra.

15 recordsLinked to original sources
PubMed2002

Apollo: a sequence annotation editor.

The well-established inaccuracy of purely computational methods for annotating genome sequences necessitates an interactive tool to allow biological experts to refine these approximations by viewing and independently evaluating the data supporting each annotation. Apollo was developed to meet this need, enabling curators to inspect genome annotations closely and edit them. FlyBase biologists successfully used Apollo to annotate the Drosophila melanogaster genome and it is increasingly being used as a starting point for the development of customized annotation editing tools for other genome projects.

Animals↗
PubMed1992

Molecular cloning and characterization of a constitutively expressed heat-shock-cognate hsc71 gene from rainbow trout.

A rainbow trout major heat-shock-protein-like gene (hsp 70) and corresponding cDNA clones were isolated by hybridization to heterologous hsp70 probes. DNA sequencing revealed that this gene is structurally similar to a mammalian heat-shock-cognate hsc70 gene and consists of eight introns. Northern blot and primer extension analyses showed that the corresponding mRNA is constitutively abundant in different trout tissues and salmonid cell lines. Fragments of the isolated gene containing the -900 - +30 and -217 - +58 sequence were linked to a bacterial chloramphenicol acetyltransferase reporter gene and transiently transfected into salmonid cells. The expression pattern of these constructs supports our conclusion that the isolated genomic and cDNA clones correspond to a trout heat-shock-cognate hsc70 gene.

Amino Acid Sequence↗
PubMed1992

Occurrence of wax esters and 1-O-alkyl-2,3-diacylglycerols in goat epididymal sperm plasma membrane.

Two unusual lipid classes were detected by thin-layer chromatography in the neutral lipids derived from goat cauda-epididymal sperm plasma membrane. The lipids were identified as wax esters and 1-O-alkyl-2,3-diacylglycerols based on chromatographic properties, identity of their hydrolysis products, and infrared/1H nuclear magnetic resonance spectral evidence. The membrane contained ca. 3 and 5 micrograms/mg protein of wax esters and alkyldiacylglycerols, respectively. The relative proportions of wax esters and alkyldiacylglycerols in the total neutral lipids were 1.5% and 2.4%, respectively. The lipids contained fatty acids with chain lengths of C14 to C22. The major fatty acids of the wax esters were 14:0, 16:0, 16:1 omega 7, 18:0 and 18:1 omega 9. The fatty acids in alkyldiacylglycerol were 16:0, 18:0, 22:5 omega 3 and 22:6 omega 3. Alkyldiacylglycerol was particularly rich in docosahexaenoic acid (22:6 omega 3) representing 30% of the total fatty acids. The alcohols of wax ester were all saturated with C20-C29 carbon chains. The deacylated products derived from alkyldiacylglycerols were identified as hexadecyl, octadecyl and octadec-9'-enyl glycerol ethers.

Animals↗
PubMed1992

Isozymic pattern of lactate dehydrogenase in cases of bancroftian filariasis.

Isozymic patterns of lactate dehydrogenase (E.C. 1.1.1.27) by polyacrylamide gel electrophoresis (PAGE) were observed in various categories of filariasis and controls, i.e. asymptomatic microfilaraemia and symptomatic amicrofilaraemia, endemic normal and non-endemic normal. Lactate dehydrogenase (LDH) activity was also observed amongst the above categories of patients. An increase in enzyme activity and change in the isozymic pattern was observed in the above categories of filaria infected serum. LDH activity doubled in asymptomatic microfilaraemia whereas in symptomatic amicrofilaraemia the increase in LDH activity was thirtyfold. The isozymic pattern of microfilaraemic cases showed the presence of three bands B4, A1B3, A2B2, which are quite thick as compared to normal healthy subjects, whereas the patients with symptomatic amicrofilaraemia showed marked elevation of serum LDH-4 or A3B1. The LDH was partially purified by combined treatment of (NH4)2SO4 fractionation and gel filtration. The isozymic pattern of purified LDH showed a similar pattern.

Animals↗
PubMed1992

A comparative study of acetylcholinesterase activity in bovine (S. cervi) and human (B. malayi, W. bancrofti) filaria.

Setaria cervi, a bovine filarial parasite, contains a significant amount of acetylcholinesterase (AChE) activity with microfilaria having five to ten times more AChE activity than female and male adult worms, respectively. Because AChE shows substrate specificity and hydrolyzes acetylthiocholine but not butrylthiocholine, this parasitic enzyme is likely a true acetylcholinesterase. The latter also resembles an AChE enzyme in the human filarial parasite B. malayi which hydrolyzes acetylthiocholine iodide three times faster than butrylthiocholine iodide. The S. cervi AChE, like its counterpart, also exhibit inhibition with eserine, a specific inhibitor of this enzyme. Subcellular localization of AChE in adult female worms shows enzyme activity both in the mitochondrial and post-mitochondrial fraction. However, enzyme activity in the soluble fraction is twenty-seven times greater than in the mitochondrial fraction.

Acetylcholinesterase↗
PubMed1992

In vitro and in vivo effect of diethylcarbamazine on the activity of acetylcholinesterase from Wuchereria bancrofti infected human serum.

The acetylcholinesterase (AChE) activity was measured in human serum from persons infected with the filarial parasite Wuchereria bancrofti. The asymptomatic microfilaremic serum showed five times increase in AChE-activity as compared with normal serum, whereas only little difference was observed in serum from patients with elephantiasis. Similar results were obtained when the enzyme activity was measured in the immune complexes precipitated with polyethyleneglycol. Further, the effect of the antifilarial drug diethylcarbamazine (DEC), on the AChE activity of infected and normal serum was studied in in vivo and in vitro experiments. In vitro, DEC was found to be effective only with respect to AChE from asymptomatic microfilaremic serum where 75% decrease in enzyme activity was observed at 100 mumol. The oral administration of DEC (5 mg/kg of body weight/day) effected the activity of AChE from microfilaremic serum as shown after 1 hr, 1 and 3 weeks. A regular decrease in enzyme activity of asymptomatic microfilaremic serum was observed. By increasing time periods and after three weeks the level of AChE reaches the normal value. In vitro and in vivo the same concentration of DEC has negligible effect on the normal serum suggesting that in case of asymptomatic microfilaremic serum the increased activity of AChE is different in nature than the host acetylcho-[abstract incomplete in journal]

Acetylcholinesterase↗
PubMed1992

The normal glenohumeral relationships. An anatomical study of one hundred and forty shoulders.

We measured the dimensions of the humeral and glenoid articular surfaces in 140 shoulders that were representative of a given population of patients, and also evaluated several glenohumeral relationships. Ninety-six measurements were made in the shoulders of cadavera and forty-four, on magnetic resonance-imaging studies of living patients. Eighty-five per cent of the humeral measurements fell within eight fixed combinations of the radius of curvature and the thickness of the humeral head, in two-millimeter increments. The average radius of curvature of the humeral head in the coronal plane was 24 +/- 2.1 millimeters (range, nineteen to twenty-eight millimeters). The average thickness of the humeral head was 19 +/- 2.4 millimeters (range, fifteen to twenty-four millimeters). There was a wide variability in the size of the humeral head and a direct correlation between the differences in size and the heights in both men and women. The humeral articular surface was spherical in the center; however, the peripheral radius was two millimeters less in the axial plane than in the coronal plane. Thus, the peripheral contour of the articular surface was elliptical (ratio, 0.92). The radius of curvature of the glenoid, measured in the coronal plane, was an average of 2.3 +/- 0.2 millimeters greater than that of the humeral head. The average dimensions of the glenoid in the superior-inferior and anterior-posterior (lower half) directions were 39 +/- 3.5 millimeters (range, thirty to forty-eight millimeters) and 29 +/- 3.2 millimeters (range, twenty-one to thirty-five millimeters). The anterior-posterior dimension of the glenoid was pear-shaped, the lower half being larger than the top half. The ratio of the lower half to the top half was 1:0.80 +/- 0.01. There was a strong linear correlation between the lateral humeral offset and the size of the humeral head (radius of curvature and thickness). The average lateral humeral offset was 56 +/- 5.7 millimeters (range, forty-three to sixty-seven millimeters). The superior most point on the humeral articular surface was an average of 8 +/- 3.2 millimeters (range, three to twenty millimeters) cephalad to the top of the greater tuberosity. Our data show that reconstruction of the lateral humeral offset is important in optimization of the moment arm of the deltoid and rotator cuff and of the normal tension of the soft tissue after prosthetic reconstruction.

Adult↗
PubMed1977

Profile of epilepsy in a developing country: a study of 1,000 patients based on the international classification.

The present prospective study undertaken in a specialized neurological center of a developing country deals with 1,000 epileptic patients classified in accordance with the International Classification. Eighty-one percent of the patients could be classified, with a lower incidence in the younger age group. Partial epilepsy was found to be far more common than generalized epilepsy (80% versus 20%). Primary generalized epilepsy was seen in 15% and secondary generalized in 5%. Partial epilepsy with elementary symptomatology was seen in 58% and complex symptomatology in 7%. Secondarily generalized seizures were seen in the remaining 15%. Primary generalized epilepsy and partial epilepsy with complex symptomatology were more common in adults. Secondary generalized epilepsy and partial epilepsy with secondarily generalized seizures were more common in children. Partial epilepsy with elementary symptomatology, however, did not vary significantly with age. The higher incidence of partial epilepsy in our patients, compared to the West, could be due to greater frequency of CNS infections and birth injuries, which are common childhood hazards in the developing countries.

Adult↗
PubMed1977

Plasma cortisol in glaucoma.

Plasma cortisol level and intraocular tension were determined diurnally in 40 normal and 40 glaucomatous patients at 8 AM and 8 PM. It was observed that (1) plasma cortisol level was higher in glaucomatous patients at both the periods; (2) the diurnal variation in plasma cortisol was parallel to diurnal variation in intraocular tension in both glaucomatous and normal persons; and (3) the diurnal variation in plasma cortisol level also was more marked in glaucomatous patients as compared to normal persons.

Adult↗