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S Morii

Publications and source records attributed to S Morii.

At least 73 records · Page 4Linked to original sources
PubMed1988

Effect of sciatic nerve stimulation on pial arterioles in rats.

The present study documents the microvascular response of the pial circulation in sensory hindlimb cortex to sciatic nerve stimulation. Rats, anesthetized with alpha-chloralose and urethan, were equipped with closed cranial windows, and pial arteriolar diameter was measured during stimulation of the contralateral sciatic nerve. The effects of varying stimulus frequency, intensity, and duration were examined. Optimal stimulus frequency was 5 Hz, but response diminished significantly beyond 10 Hz. Optimal stimulus intensity was 0.2 V. At higher stimulus strength, arteriolar dilation was reduced, but systemic blood pressure rose significantly. At low stimulus frequency and intensities, pial arterioles responded to stimulation with a consistent pattern: initial delay of 1.4 s followed by abrupt dilation to a peak magnitude, subsequent decline to a lesser but still dilated state, and recovery to a resting diameter after the cessation of stimulation. No consistent response profile was discernible at high stimulus intensity and/or frequency. This vasodilatory response was discretely restricted to a limited number of arterioles, confined to the hindlimb somatosensory cortex as confirmed by sensory evoked response. The response of the pial circulation provides a well-characterized model for analysis of brain microcirculation, which presumably is linked to cerebral metabolism.

Animals↗
PubMed1988

[Apocrine carcinoma of the breast--a morphologic comparison of the apocrine sweat glands and the apocrine metaplastic epithelia in mastopathy].

An operatively removed apocrine carcinoma of the breast from a 62-year-old Japanese lady has been observed by the ABC method, using the monoclonal antibody 115D8. The cancer cells and metaplastic epithelia exhibited similar ultrastructural findings (an apical snout, apocrine granules, etc.) as the apocrine sweat gland cells, although no evidence of apocrine secretion could be detected. The immunohistochemical testing, using monoclonal antibody, 115D8, showed an apical, linear, dot-like, staining in the supranuclear regions on the apocrine sweat gland cells and on the apocrine metaplastic cells of the mammary gland. Similar stainability also was observed in the well-differentiated area of the apocrine carcinoma, while a heterogeneity in staining, such as unstained cells and diffuse cytoplasmic-stained cells were found in the poorly-differentiated areas. These abnormal staining patterns indicate the malignant changes of the apocrine cells.

Antibodies, Monoclonal↗
PubMed1987

Immunohistochemical demonstration of MAM-3 and MAM-6 antigens in normal human skin appendages and their tumors.

The expression of MAM-3 and MAM-6 antigens was immunohistochemically investigated on 110 tumors of human skin appendages. Forty-two samples from tumor-adjacent normal skin appendages were also studied. MAM-3 antigens, as detectable by monoclonal antibodies (MoAbs) 67D11, 115G3, and 115H10 were present in the inner layer cells but not in the outer layer cells of normal eccrine excretory ducts. Sporadic positivity was also found in cytoplasm of apocrine acini with 115G3, while 67D11 and 115H10 were negative. MAM-6 antigens, as detectable by the MoAbs 115D8, 115F5, 139H2, 140C1, and 126E7 were found in the secretory canaliculi of normal eccrine acini and within the apical lumina at the terminal portion of ducts. Apocrine acinar cells mainly exhibited an apical staining, but a focal supranuclear dot-like staining could also be observed. A foamy reaction pattern for MAM-6 was noted in mature sebocytes. However, none of the antigenic epitopes was expressed in normal squamous epithelium or hair follicles. In benign tumors, the staining patterns for both antigens, in general, resembled their distribution in the corresponding normal tissues. However, carcinomas originating from sweat glands, sebaceous glands, and the pilar apparatus expressed both antigens in a more irregular and heterogeneous pattern. This might preferably be explained by the loss of those mechanisms controlling the antigen expression in mature, functional tissues. Conclusions from these immunohistochemical studies with regard to the histogenesis mainly of the malignant skin appendage tumors should be drawn with caution.

Antibodies, Monoclonal↗
PubMed1987

Immunoelectron microscopic localization of mammary tumor virus (MTV) antigens in normal and cancerous mammary glands of mice.

Peroxidase-labeled Fab' fragments of rabbit antisera against gp52 (major envelope protein) and A-particles of mammary tumor virus (MTV) were prepared and used for investigation by immunoelectron microscopy of the replication cycle of MTV-specific envelope and core antigens in normal and malignant mammary gland cells of female mice. The specificity of the antisera was proven by absorption tests and lack of reactivity to MTV-free mammary tissues. Periodate-lysine-paraformaldehyde (PLP) fixation sufficiently preserved the antigenicity of gp52, while Zamboni's fixative was useful to preserve the core antigen. Saponin pretreatment was necessary to reveal the intracellular antigen of A particles but had no influence on gp52. In addition to its presence at the envelope of D particles, gp52 was clearly associated with the biomembrane system, including the nuclear membrane, endoplasmic reticulum, Golgi apparatus and plasma membrane independent of the presence of virus particles. In mammary tumors, a significant level of gp52 antigen was often expressed on the entire cell surface membrane. In contrast, it was localized only to the apical plasma membrane in normal mammary gland cells. A particle antigens were confined to the intracytoplasmic A particles, usually visible as clusters, and to the inner part of B particles. These ultrastructural findings support the available biochemical data on the morphogenesis of MTV particles.

Animals↗
PubMed1987

Systemic amyloidosis with a mesenteric mass which had the appearance of proteinaceous lymphadenopathy.

An autopsy case of systemic amyloidosis in a 46-year-old Japanese female who had M component in sera but no manifestations of myeloma is described. Autopsy revealed massive milky ascites and a large mesenteric mass, which showed histologically perivascular concentric deposition of eosinophilic materials with mixture of residual follicles and scattered plasma cells. These morphological findings closely resembled those seen in proteinaceous lymphadenopathy, and the material in the mesenteric mass proved to be an amyloid A lambda protein. Subsequent immunohistochemical observations strongly suggested that this mass lesion was a "burnt-out" plasmacytic dyscrasia.

Amyloidosis↗
PubMed1987

Role of adenosine in regulation of cerebral blood flow: effects of theophylline during normoxia and hypoxia.

We studied the effects of the methylxanthine theophylline, an adenosine receptor blocker, on cerebral circulation. Cerebral blood flow (CBF) was measured by the retroglenoid outflow and microsphere techniques, and pial circulation changes were observed through a closed cranial window. Intraperitoneal administration of theophylline in normoxic animals resulted in a biphasic response of pial vessels and CBF. At low concentrations (0.05 mumol/g) of theophylline, pial vessel diameter and CBF decreased, whereas vasodilatation and hyperemia were observed at higher levels. After intraperitoneal administration of either 0.05 or 0.2 mumol/g, hypoxic hyperemia was attenuated both during short (c. 30 s) and sustained (c. 2-3 min) hypoxia, as was hypoxic pial arteriolar vasodilatation. These actions of theophylline appear to be due to adenosine receptor blockade, since micromolar concentrations were achieved in cerebrospinal fluid (CSF), and no increases in adenosine 3',5'-cyclic monophosphate concentrations in brain were noted. Moreover, theophylline (either intraperitoneal or topical) blocked pial vasodilatation caused by topically applied adenosine, but had little effect on hypercarbic hyperemia or pial vasodilatation induced by topically applied acetylcholine. The results of these studies suggest that adenosine is involved in the maintenance of resting cerebral vascular tone and has a paramount role in the regulation of CBF during hypoxia.

Adenosine↗
PubMed1987

Mouse mammary tumor virus (MMTV) particles in extraorbital lacrymal and urethral glands of endogenous MMTV-carrying mice.

Expression of mouse mammary tumor virus (MMTV) antigens was observed in a wide variety of exocrine glands in adult mice by an immunoperoxidase method using rabbit antisera against gp52 and p27 of MMTV. Both exogenous and endogenous MMTV-carrying SHN, GRS/A, C3H and DDD-Mtv-2 mice expressed both antigens in the mammary, extraorbital lacrymal, urethral, parotid and submandibular glands of both sexes and prostate, seminal vesicle and epididymis but not in the Harder's, Zymbal's sublingual and uterine glands. Endogenous MMTV-free but exogenous MMTV-carrying BALB/cfC3H mice showed antigen expressions only in the mammary gland. BALB/c mice free from either endogenous or exogenous MMTV did not express viral antigens in any tissues examined. Electronmicroscopically, MMTV A and B particles were detected in the above mentioned MMTV antigen positive non-mammary organs in endogenous MMTV-carrying mice. The results suggested that the endogenously expressing Mtv genes might produce mature MMTV in these non-mammary glandular systems.

Animals↗
PubMed1987

[An autopsy case of an extranodal malignant lymphoma occurring primarily in the chest wall].

This case history involves a 72-year-old male in whom an artificial left pneumothorax had been induced 46 years earlier. Two years ago a subcutaneous tumor was uncovered in his left chest wall, which was reduced with radiation and chemotherapy. Abnormal shadows began to appear in the left lung 5 months prior to his death. On autopsy, tumors were found to have developed in the left pleural cavity and lung, but no tumor cells had infiltrated to the lymph nodes, the liver, the spleen, or the bone marrow. Tumors that were examined by a biopsy or seen on autopsy revealed a diffuse large cell lymphoma, the T cell type. All 7 reported cases in Japan of a primary lymphoma in the thorax have shown a past history of tuberculous pleurisy and/or pyothorax after a pneumothorax.

Aged↗
PubMed1986

Reactivity of rat pial arterioles and venules to adenosine and carbon dioxide: with detailed description of the closed cranial window technique in rats.

This study describes a closed cranial window technique that allows the observation and measurement of rat pial arterioles and venules in situ. The resolving power of this system is 1-2 microns. Using this sensitive technique, we characterized the responses to 7% carbon dioxide inhalation and adenosine in arterioles (10-70 microns) and venules (15-100 microns). During carbon dioxide inhalation, larger arterioles (greater than 40 microns) dilated more than smaller arterioles (less than 20 microns). There was limited vasoreactivity of pial venules during CO2 inhalation. Dilation of arterioles was initially observed with an adenosine concentration of 10(-8) M. Almost a twofold increase in diameter was noted at 10(-3) M. In contrast to the effect of CO2 inhalation, the degree of dilation with topical application of adenosine was not size dependent. Pial venules did not respond to adenosine. The technique for observation of pial vessels using the closed cranial window and for measurement of vessel diameter by video camera system microscopy is a powerful tool for studying in vivo the cerebral circulation in the rat.

Adenosine↗
PubMed1986

Immunohistochemical expression of mammary tumor virus antigens in mammary glands of virgin mice, in relation to Mtv genes.

MTV antigens in the resting mammary glands of GRS/A, SHN, C3H, and Balb/c virgin mice were detected by immunoperoxidase techniques using antiserum against MTV whole virion, gp 52 or p 25 to differentiate the expression between endogenous and exogenous MTV. Balb/c mice were crossed to infect MTV into each reciprocal hybrid or fosternursed inbred. Immunohistochemical stainings of gp 52 in the formol-fixed sections were almost the same as those of the whole MTV virion, and the results on various cases were as follows: In the mice with endogenous GR-MTV, positivity was first observed at the age of 14 days, while the first expression of exogenous GR-MTV was delayed to the age of 140 days. The mice with endogenous and/or exogenous SHN-MTV showed the first antigen appearance at the age of 65 days, and those with exogenous C3H-MTV did at the age of 80 days. The virgins with only endogenous C3H-MTV came to express the antigen after the age of 200 days. Staining of p 25 in Carnoy-fixed sections of MTV-positive mammary glands was found in the supranuclear cytoplasm and apical surface of the glandular cells and the lumen, all of which are the site of A and B particles. By means of preembedding method for gp 52, the reaction products were ultrastructurally detected not only on the MTV-budding apical surface, together with the intraluminal B particles, but also on the MTV-free apical cell membrane of the glandular cells in the mammary gland of the GR virgins.

Animals↗
PubMed1986

A venous outflow method for continuously monitoring cerebral blood flow in the rat.

We analyzed the retroglenoid venous outflow (VOF) technique in the rat to document the validity of this method of measuring cerebral blood flow (CBF). Stereotypic changes in CBF were obtained with VOF during hypercarbia and hypotension. O2 content of retroglenoid venous blood did not differ significantly from O2 content of blood obtained from the sagittal sinus, suggesting minimal extracerebral contamination of the retroglenoid venous blood. This lack of extracerebral contamination was further analyzed using a double tracer technique (125I-labeled serum albumin, 22Na) that quantitated minimal extracerebral contamination in the retroglenoid vein. CBF measurements were made simultaneously using microsphere and VOF methods, and excellent correlation was found between the two techniques over a wide range of CBF during normoxia, hypoxia, and normoxic hypocarbia and hypercarbia. However, a decrease in the ratio of VOF to microsphere CBF was observed during severe normoxic hypotension (mean arterial pressure = 41 +/- 4 mmHg). VOF represented 18% of total CBF as measured by microsphere method. This study indicates that the retroglenoid outflow technique in rats is a valid method of measuring CBF.

Animals↗
PubMed1986

Immunoreactive prolactin in mouse urethral glands.

Immunoreactive prolactin was demonstrated in the acinar cells of urethral glands in some inbred strains of mice by immunoperoxidase technique. The significance of the presence of prolactin or its related substances in the urethral glands in relation to their role in the urinary tract is discussed.

Animals↗
PubMed1986

[An autopsy case of epithelioid sarcoma--a review of the cases reported in Japan].

A 48-year-old Japanese woman died of a general metastasis of epithelioid sarcoma originating in the right palm, which had been irradiated for trichophytia therapy 30 years earlier and where a contraction of the fingers and recurrent ulceration for seven years despite several surgical treatments had occurred. Although the tumor cells in the biopsy materials consisted of both large ovoid and polygonal cells with deeply eosinophilic cytoplasm and spindle-shaped cells, no particular appearances for definite diagnosis were obtained. The epithelioid appearance of tumor cells was first recognized in the metastatic foci of the lungs and kidneys at autopsy. In addition, 20 cases of epithelioid sarcoma reported in Japan are reviewed.

Female↗
PubMed1985

Expression of MAM-3 and MAM-6 antigens in endometrial and endocervical adenocarcinomas.

Expression of milkfat globule membrane antigens (MAM-3, MAM-6) was investigated by an indirect immunoperoxidase method on normal and malignant glands of the endocervical and endometrial tissues. MAM-3 antigens, as detectable with monoclonals 67D11 and 115G3, were not detected in normal endometrial glands, but they were found in the majority of endometrial adenocarcinomas. In the endocervix, MAM-3 antigens were present in almost all of the normal glands and in the cancerous glands. MAM-6 antigens, as detectable with monoclonals 115D8 and 115F5, were observed in majority of normal and neoplastic glands within the endometrium and endocervix. The staining appearance of the antigens was generally heterogenous in the neoplastic tissues but more homogenous in normal glandular tissues. These results are discussed and compared with the grade of histological differentiation, mucin production and positivity for CEA.

Adenocarcinoma↗
PubMed1985

The role of adenosine in autoregulation of cerebral blood flow.

We have investigated the role of adenosine, a purine nucleoside and potent vasodilator of cerebral pial vessels, during both acute (0-60 sec) and sustained (2-5 min) changes in cerebral perfusion pressure. Brain adenosine concentrations are rapidly increased within 5 sec of the onset of systemic hypotension and parallel, in a temporal fashion, the changes in pial vessel diameter and alterations in cerebral vascular resistance. During sustained hypotension, brain levels of adenosine are increased even within the autoregulatory range. These data are constant with the hypothesis that adenosine is an important metabolic factor in cerebral autoregulation.

Adenosine↗
PubMed1985

Effects of long use of a beta-blocker, carteolol, on exercise hemodynamics in hypertensives.

The symptom limited maximal exercise was performed by a supine bicycle ergometer before and 12 weeks after oral carteolol therapy in 10 patients with essential hypertension of WHO Stage 1 and 2. At rest, arterial blood pressure (BP) and venous pressure (VP) significantly declined after therapy, while heart rate (HR), cardiac index (CI), stroke index (SI) and systemic vascular resistance (SVR) were not significantly changed. The maximal exercise work of post-therapy was similar to that of pre-therapy in 9 patients and increased in one. When compared with the pre-therapy values at each work load of exercise, carteolol caused significant decreases in systolic BP, mean BP, HR and CI, and a significant increase in SVR, while SI and VP were not significantly changed. Plasma levels of renin activity, aldosterone, and catecholamines were not altered by carteolol throughout the study at rest and with exercise. It may be concluded that the inhibition of HR increase by carteolol results in a reduced cardiac output during moderate and severe exercise and then suppresses the elevation of BP, so that carteolol makes it possible to achieve more exercise with less cardiac work.

Administration, Oral↗