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Biomedical subjects

S Ochs

Publications and source records attributed to S Ochs.

At least 91 records · Page 5Linked to original sources
PubMed1970

Metabolic dependence of fast axoplasmic transport in nerve.

Fast axoplasmic transport, shown in cat sciatic nerves by a crest of labeled activity after injection of the L7 ganglion with [(3)H]-leucine or [ (3)H]-lysine, was stopped within 15 minutes after death of the animals by bleeding. If the sciatic nerves were removed from the animals and placed in a chamber supplied with oxygen at 38 degrees centigrade, fast transport was sustained. Transport was rapidly blocked in similar in vitro preparations when the nerves were kept in a nitrogen environment. Fast axoplasmic transport is closely dependent upon oxibdative metabolism.

Animals↗
PubMed1969

Fast transport system of materials in mammalian nerve fibers.

A fast transport system of materials is shown in cat sensory fibers of sciatic nerve. After injection of tritiated leucine into the lumbar seventh ganglia, the distribution of activity was measured in the sciatic nerves from 2 to 8 hours afterward. The distribution showed a crest of activity advancing down the fibers at a rate of approximately 410 millimeters per day. An intraaxonic locus of the activity was indicated by a block of the downflow induced by local freezing which causes the fibers to close off. Some of the activity in the nerve was due to free tritiated leucine, with most of it incorporated into polypeptide, soluble protein, and subcellular particulates.

Animals↗
PubMed1966

Action of choline on frog sartorius muscle.

1. External and micro-electrode measurements were made of fibre membrane potentials taken at intervals along the length of frog sartorius muscle fibres before and after adding choline. In ;cold-treated' Rana pipiens which had been kept at 1-3 degrees C for at least 5 days, 10(-3) w/v choline caused a long-lasting depolarization of approximately 3 hr. Depolarization occurred first at the end-plate region and spread more slowly to the end-plate free region. The potential became equalized along the muscles during recovery.2. R. pipiens kept at room temperature showed a faster repolarization in the choline solution, within approximately 1 hr. R. temporaria showed a similar recovery time in muscles from ;cold-treated' animals. In R. temporaria kept at room temperature the duration was shorter, approximately 20-30 min. The apparently generalized depolarization of the muscle seen in ;cold-treated' R. pipiens appears to be due to the spreading effect of a long-maintained depolarization at the end-plates. A similar far-spread of depolarization was seen following crush of fibres.3. Length-constants of muscle fibres determined with pulses were found to be within the normal range in muscles from ;cold-treated' R. pipiens and slightly reduced after adding choline.

Animals↗
PubMed1966

Effects of sodium extrusion and local anaesthetics on muscle membrane resistance and potential.

1. Membrane potentials and resistances of K-depleted muscles were measured in the cold and again after warming in K-containing media so that active ion movements occurred.2. On warming there was a fall of resistance and a gradual rise of potential which passed through a maximum. Later measurements of resistance in a chloride medium showed that values were, if anything, higher than initially in the warm.3. The excess potentials measured approximated to those required to induce passive inward movement of the K ions through the measured K resistance.4. Permeabilities for K(+) and Cl(-) were deduced. When cocaine, procaine, amytal or mepyramine were added or when K(+) was replaced by Rb(+) in the Cl(-)-free solution the K(+) permeability was eventually reduced. The same agents led to an enhanced initial response of potential to warming, but later the potentials in Cl(-)-free media fell to less than the K(+) equilibrium values.5. A method for obtaining the resistivity of the membrane from measurements made in conditions of non-linear voltage-current dependence was applied.

Amobarbital↗