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Biomedical subjects

S Ohmori

Publications and source records attributed to S Ohmori.

At least 271 records · Page 15Linked to original sources
PubMed1983

A simple and specific colorimetric determination of cysteine with p-dimethylaminocinnamaldehyde.

A simple and specific assay was developed for the determination of cysteine. Cysteine was reacted with p-dimethylaminocinnamaldehyde in methanol at 60 degrees C for 2 h in the presence of sulphuric acid as a catalyst. Absorbance was measured at 587 nm; the chromogen was stable at 60 degrees C for at least 5 h. The colour reaction was specific for cysteine, and was negative for other amino acids but positive for cysteamine. The absorbance followed Beer's law in the range from 0.825 to 413 mumol/l, and the apparent molar lineic absorbance was calculated to be 5000 m2 . mol-1. This procedure is applicable to the determination of cysteine and cystine in amino acid mixtures and in plasma, since cystine can be quantitatively reduced to cysteine with dithiothreitol under alkaline conditions. Recovery of cysteine and cystine from human plasma was 98.5 to 103% and 94.2 to 102%, respectively.

Chromatography, High Pressure Liquid↗
PubMed1982

Relationship between monensin and sulphur amino acid requirements in broiler chickens.

1. Diets containing 80, 100, 125, 150, 175 or 200 mg monensin/kg were fed to broiler chickens from 0 to 28 d in cages that prevented access to excreta. 2. Growth was depressed with 125 mg monensin or more/kg and food intake tended to decrease. Feathering was adversely affected with 175 mg or more/kg. 3. In a similar experiment, diets containing 8.8, 9.1, 9.4, 9.7 and 10.1 g total sulphur amino acids (SAA)/kg were supplemented with 125 or 80 mg monensin/kg and compared with a diet containing 8.8 g SAA and 33 mg robenidie/kg. 4. With 125 mg monensin/kg, body-weight gain was significantly less than that of birds receiving robenidine if dietary SAA content was 9.4 g or less/kg. With 9.7 g SAA or more/kg, gain in birds receiving monensin was similar to that of birds receiving robenidine. 5. Monensin at 125 mg/kg therefore appears to increase SAA requirement.

Amino Acids, Sulfur↗
PubMed1982

[Determination of bromine contents in the blood and hair of workers exposed to methyl bromide by radioactivation analysis method].

The bromine contained in blood and hair samples obtained from workers exposed to methyl bromide was analyzed by non-destructive activation analysis. The samples were irradiated for 1 min in pneumatic irradiation facility of Kyoto University research reactor with an estimated thermal neutron flux density at 2 X 10(13)n X cm-2 X sec-1. The irradiation was counted with Ge(Li) detector. The duration of measurement of radionuclide bromine in blood and hair was 200 sec within 2-10 min after irradiation. The bromine content of serum sample obtained from a worker suspected of methyl bromide poisoning was found to be 412 micrograms/g on 13 days apart from exposure to methyl bromide. The biological half-life of bromine in this case was found to be about 16 days. Then bromine contents in serum and hair samples obtained from workers exposed to methyl bromide were found to be 1.7 and 2.6 times higher respectively than those of nonexposed workers. Any correlations of bromine contents were not observed between serum and hair samples obtained from workers exposed to methyl bromide, nor between bromine amount in the serum of workers exposed to methyl bromide, and the terms exposed to it, but statistically significant positive correlation was observed between bromine amount in the hair of workers exposed to methyl bromide and the terms exposed to it.

Adult↗
PubMed1981

Recent progress in the treatment of portwine staining by argon laser: some observations on the prognostic value of relative spectro-reflectance (RSR) and the histological classification of the lesions.

Over a period of more than three years 1264 patients with a portwine stain have been treated by argon laser. This form of therapy is more effective and worthwhile than the other procedures we have used in the past. The histological classification of the various types of portwine stain and the measurements of Spectro-reflectance (RSR) obtained by the spectrophotometric colour difference meter (Murakami) provide valuable and reliable prognostic criteria for this form of treatment.

Adolescent↗
PubMed1981

An NADP-dependent 3 alpha-hydroxysteroid dehydrogenase of rat liver active against C19, C20, C23, C24, C25 and C26 steroids.

Rat liver cytosol was previously shown to contain at least three NADPH-dependent enzymes which catalyze the hydrogenation of chloral hydrate. Two of them had previously been shown to catalyze the hydrogenation of long-chain aliphatic and aromatic aldehydes or aromatic ketones in addition to halogenated acetaldehydes. The present paper demonstrates that one of these enzymes also catalyzes the reversible hydrogenation of 3-oxosteroids to 3 alpha-hydroxysteroids. We confirmed that the enzyme catalyzes the reversible hydrogenation of C23, C24 and C26 3-oxo bile acids with a 1:1 stoichiometry. The substitution at C-12 with a hydroxyl group markedly decreased the reaction rate, and the rate of dehydrogenation of C20, C23, C26, C24, and C25 bile acids decreased in the descending order. In general, the conjugation with glycine or taurine increased the reaction rate, while the replacement of the terminal carboxyl group of the C24 compound with an isobutyl group markedly decreased it. 3 alpha-Hydroxy bile acids with A/B trans configuration were also preferable substrates for the enzyme, although the reaction rate was relatively low compared with that of those with A/B cis configuration. The enzyme may be involved in the biosynthesis of lithocholic acid from cholesterol through 3 alpha-hydroxy-5-cholen-24-oic acid, which was proposed by Mitropoulos and Myant (Mitropoulos & Myant (1967), Biochem. J. 103, 472--479).

3-Hydroxysteroid Dehydrogenases↗
PubMed1980

Improved gas chromatographic method of determining diclofenac in plasma.

Diclofenac was converted into either its methyl or ethyl ester in methanol or ethanol containing 0.1% or 0.5% sulfuric acid, respectively. The ester was extracted and subjected to gas--liquid chromatography with electron-capture detection. The esterification resulted in an increased sensitivity of the gas chromatographic detection, three times better than that previously reported for the formation of indolone ring in trifluoroethanol containing 0.5% sulfuric acid.

Chromatography, Gas↗
PubMed1980

Effect of propranolol on glucose disposal rate in patients with hyperthyroidism.

The effect of propranolol treatment (60 mg per day, three days) on glucose disposal rate (K-value) was investigated in nine patients with hyperthyroidism. K-value was improved in 4 cases and aggravated in 5 cases. The fasting levels of plasma free fatty acids (FFA) before the administration of propranolol in the improved cases were significantly higher than those in the aggravated cases. The propranolol treatment markedly reduced FFA levels only in the improved cases. These results suggest that the impaired glucose tolerance frequently seen in hyperthyroidism patients could be partly attributed to the increased level of plasma FFA.

Adult↗
PubMed1980

Colorimetric determination of glycine conjugates of bile acids.

A simple assay was developed for the determination of glycine-conjugated bile acids. Samples containing the glycine-conjugated bile acids in the range from 15 to 250 nmol were acidified with HCl, and extracted with ethyl acetate containing ethanol (50 ml/l). An aliquot of the organic phase was evaporated to dryness, and the dried residue was treated to develop the color by the addition of acetic anhydride, pyridine and a trace amount of phosphoric acid. the absorbance was measured at 429 or 456 nm after the reaction at 50 degrees C for 2 h. Color development did not occur with unconjugated and taurine-conjugated bile acid. Beer's law was obeyed from 12.5 to 200 nmol in a cuvette. The recovery of the conjugates from the rabbit gall bladder bile and liver homogenate was satisfactory. This method requires no hydrolysis step and is applicable to the determination of glycine-conjugated bile acids in bile, duodenal aspirate and liver homogenate.

Animals↗
PubMed1979

Reconstruction of the auricle in minor grades of microtia by a silicone framework.

A silicone ear frame was used in a series of 31 minor microtia cases for subtotal external ear reconstruction. The authors' original method is described and includes the unfolding of the microtic ear in 3 flaps, circular shape trimming of the frame, and enlargement of the conchal cavity. There have been no failures in this series and no exposure in up to 4 years follow-up. Aesthetic results, on the whole, have been excellent.

Adolescent↗