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S Partanen

Publications and source records attributed to S Partanen.

At least 55 records · Page 3Linked to original sources
PubMed1983

Simultaneous azo-coupling method for steroid acetate hydrolyzing enzyme.

A simultaneous azo-coupling method for histochemical localization of steroid acetate hydrolyzing enzyme is described. It is based on the observation that d-equilenin, a natural oestrogenic steroid hormone, forms deeply coloured insoluble reaction products with diazonium salts under reaction conditions suitable for histochemical purposes. An acetate at position 3 of d-equilenin is rapidly hydrolysed by tissue esterase and the liberated d-equilenin couples with a diazonium salt to form a coloured precipitate. Steroid acetate hydrolyzing enzyme activity was observed in various tissues of the rat; a comparison with nonspecific esterase activity using alpha-naphthyl acetate as substrate suggested that steroid acetate hydrolyzing enzyme activity represents the activity of one or several isozymes of classical nonspecific esterase. This conclusion has also been drawn previously from biochemical studies using esters of other steroids.

Animals↗
PubMed1983

Chromosome 1q+ in erythroid and granulocyte-monocyte precursors in a patient with essential thrombocythemia.

A 55-year-old man with essential thrombocythemia had multiplication of bands q21 to q32 of chromosome 1 in all studied mitoses from bone marrow, from unstimulated blood, and from erythroid and granulocyte-monocyte colonies grown in vitro. The multiplication was in the form of triplication in 16 out of 20 mitoses from the marrow and in 4 of 6 mitoses from the blood; the rest showed a duplication of this region. All 20 mitoses from erythroid and granulocyte-monocyte colonies showed the abnormality in the form of duplication. These findings indicate most probably a clonal evolution, the triplication having arisen in the clone with the duplication. This may be associated with early leukemic transformation. The detection of the 1q+ aberration in two different types of hematopoietic colonies indicates the involvement of multipotent stem cells in at least this patient with essential thrombocythemia.

Adult↗
PubMed1983

Gastrin-producing ovarian mucinous cystadenoma.

We report a patient with abdominal pain, diarrhea, and edema of the small intestine all of which disappeared after resection of an ovarian mucinous cystadenoma of borderline malignancy. Preoperatively, the serum gastrin level was high and it decreased to normal afterwards; subsequent immunohistochemical studies of the tumor revealed cytoplasmic hormone granules containing gastrin-like immunoreactivity. We believe that the symptoms in our patient were the result of an ectopic ovarian gastrin-producing tumor.

Adult↗
PubMed1983

Spontaneous erythroid colony formation in erythrocytosis.

Erythroid colony formation from the bone marrow and blood of 30 patients with erythrocytosis has been studied. Seventeen patients formed spontaneous erythroid colonies. They included patients with polycythaemia vera (PV) and 4 patients who did not quite fulfil the Polycythemia Vera Study Group criteria for PV, but had suggestive evidence of a myeloproliferative disorder. Thirteen patients did not form spontaneous colonies. They included patients with secondary polycythaemia, patients with normal total red cell volume and patients with absolute pure erythrocytosis of unknown origin. All patients with PV, and none with secondary polycythaemias, had spontaneous erythroid colony formation. Three of 4 untreated and 2 of 9 treated PV patients had increased number of CFU-E colonies in bone marrow. From these results it can be concluded that erythroid progenitor cell culture is a useful differential diagnostic method in erythrocytosis patients who cannot be classified clinically under PV or secondary polycythaemias.

Adult↗
PubMed1983

Clonal karyotype abnormalities in erythroid and granulocyte-monocyte precursors in polycythaemia vera and myelofibrosis.

To determine whether clonal karyotype abnormalities seen in myeloproliferative disorders originate in cells of only one or of more blood cell lines, committed erythroid and granulocyte-monocyte progenitor cells were cultured in vitro by the methyl cellulose assay, and chromosome studies were carried out on the colonies. 3 patients were studied, 1 with polycythaemia vera and 2 with myelofibrosis, which was preceded by polycythaemia vera in 1 of the cases. All analysable karyotypes from both erythroid and granulocyte-monocyte colonies in every patient showed the clonal karyotype aberration which had been demonstrated in the bone marrow or blood of the patient. This finding is evidence of the involvement of a multipotent stem cell in the clonal proliferation of these diseases.

Aged↗
PubMed1983

Haematopoietic progenitors in essential thrombocythaemia.

Colony formation by haematopoietic progenitors from the bone marrow and blood of 4 patients with essential thrombocythaemia was studied in vitro using the methyl cellulose assay. 3 patients had clearly elevated numbers of BFU-E in bone marrow. 1 patient also had markedly increased numbers of CFU-E and CFU-GM, whereas the other patients had only marginally increased or normal numbers of these progenitors in the marrow. 3 patients showed markedly increased numbers of all progenitors in peripheral blood. All 4 patients showed spontaneous erythroid colony formation by progenitors from the bone marrow and 2 had spontaneous colony formation by progenitors from the blood. We conclude that essential thrombocythaemia shows abnormal colony formation in line with other myeloproliferative syndromes.

Adult↗
PubMed1983

Peripheral blood cells in the study of chromosome aberrations of patients with chronic myeloid leukaemia or myelofibrosis.

Chromosome studies were performed on peripheral blood (PB) cells with and without stimulation, and/or on bone marrow (BM) cells from 21 patients with chronic myeloid leukaemia (CML), and 18 patients with myelofibrosis (MF). Our results show that almost all the patients with immature granulocyte precursors in PB also had mitotic cells in their unstimulated PB. In CML all unstimulated mitoses had the Philadelphia chromosome. In each patient the abnormal karyotype in the PB was the same as in the BM. Because of the high frequency of dry taps in myelofibrosis, the tissue of choice for chromosome study is peripheral blood.

Blood Cells↗
PubMed1983

Mediastinal tumors: ultrastructural and immunohistochemical evaluation of intermediate filaments as diagnostic aids.

The histogenesis of six mediastinal tumors was investigated ultrastructurally and immunohistochemically using monospecific antibodies against intermediate filament proteins. Four of the tumors, showing different appearances by light microscopy, displayed desmosomes and cytoplasmic tonofilaments, by electron microscopy, compatible with an epithelial thymoma. These cases also showed keratin positivity by immunofluorescence microscopy. One spindle cell tumor showed zonula adherens-type junctions, prominent collections of intermediate filaments, and abundant cytoplasmic neurosecretory granules consistent with a neuroendocrine tumor. In this tumor, neurofilaments could be demonstrated by immunofluorescence microscopy, a feature also consistent with a neuroendocrine tumor. One malignant tumor, lacking tonofilaments and desmosomes but showing a few primitive junctions, did not contain keratin but showed vimentin positivity. This suggests a mesenchymal origin and a diagnosis of primitive sarcoma. These cases illustrate the diagnostic usefulness of electron microscopy and immunohistochemical evaluation of intermediate filaments.

Adolescent↗
PubMed1982

The resistance of glyoxylic acid induced catecholamine fluorescence to sodium borohydride reduction.

The borohydride reduction of glyoxylic acid induced fluorescence in noradrenergic and DOPA-minergic nervous structures and in amines in model experiments was studied. Both DOPAmine and noradrenaline fluorescences were resistant to borohydride reduction differing thus from the formaldehyde-induced fluorescence. Thus when the specificity of glyoxylic acid induced fluorescence is in doubt, other tests than borohydride reduction of the fluorescence must be employed.

Animals↗
PubMed1982

Histochemically demonstrable enzyme activities and their independence of the hormone receptor content in female breast carcinoma.

Acid phosphatase, leucine aminopeptidase, monoamine oxidase and non specific esterase activities were histochemically demonstrated in specimens derived from 15 infiltrating ductal carcinomas of female breast. The relative areas occupied by the enzyme-positive carcinoma cells were visually estimated and, in the cases of leucine aminopeptidase, assessed morphometrically. All enzyme activities were found to be subject to major variations within a single carcinoma and between individual carcinomas, and the activity of any single enzyme was independent of that of three others. None of the enzyme activities correlated with the estrogen and progesterone receptor values, nor the histological grade of malignancy of the tumour. Thus, histochemically demonstrable enzyme activities seem to be of no use in predicting the hormone receptor content in infiltrating ductal carcinomas of the female breast.

Acid Phosphatase↗
PubMed1982

Circulating haematopoietic progenitors in myelofibrosis.

We studied circulating erythroid and granulocyte-monocyte progenitors in 18 patients with idiopathic myelofibrosis and in healthy controls, using the methyl cellulose assay. 9 of the patients had been splenectomized prior to the study. The median number of circulating erythroid burst-forming units (BFU-E) was 8 times higher than that of the controls. 12 patients also had CFU-E (colony-forming unit, erythroid) in the blood. 10 patients had spontaneous BFU-E colony formation, and 8 patients had spontaneous CFU-E colony growth. Granulocyte-monocyte progenitors (CFU-GM) were increased 47 times compared to the controls. There were no differences in colony numbers between splenectomized and non-splenectomized patients. We conclude that in myelofibrosis, circulating erythroid and granulocyte-monocyte progenitors are usually markedly increased in number, but erythroid precursors to a lesser extent than granulocyte-monocyte precursors. Many patients, but not all, show spontaneous erythroid colony formation.

Adult↗
PubMed1981

Argyrophilic cells in carcinoma of the female breast.

Argyrophilic hormone storage granules were sought in the tissue specimens obtained from 52 benign breast lesions (13 normal breasts, 27 cases of fibrocystic disease, 9 fibroadenomas, 2 intraductal papillomas, and 3 cases of gynecomastia) and from 90 adenocarcinomas of the female breast. No argyrophilic cells were found in the normal breast tissue or in the benign lesions studied. In three of the carcinomas (3.3%) such granules were found in the tumor cells. Using electron microscopy, the argyrophilic granules were shown to be of moderate or high electron density with an average diameter of 165 to 170 nm. Ectopic hormone production was not observed clinically in any of these three patients. The absence of argyrophilic cells in normal and benign ductal and acinar epithelium, and their occasional presence in breast carcinomas favors the concept of the histogenesis of these cells through genomic derepression during the course of neoplastic transformation.

Adenocarcinoma↗
PubMed1980

Light microscopical and fluorescence histochemical observations on the endocrinological activity of human pulmonary neoplasia.

Light microscopical histochemical methods known to demonstrate the hormone storage granules of the hypophyseal ACTH/MSH cells were applied to study 14 oat cell carcinomas of human lung with special reference to assessment of the applicability of these techniques in the detection of the reported endocrinological activity of these tumors. Lead-hematoxylin, masked metachromasia and PAS reactions gave negative results for hormone storage granules in all cases studied. Argyrophilic granules were found in two tumors. The above methods, thus, were interpreted to be far too insensitive to be applicable for the demonstration of endocrinological activity. Fluorescence histochemical demonstration of the NH2-terminal tryptophyl-peptides, shown to be present in the hormone storage granules of the hypophyseal ACTH/MSH cells, yeilded positive results in three of the five studied cases. Two of these positive cases were found to have a pronounced adrenal hyperplasia. Conclusion was drawn that the used fluorescence histochemical technique for the demonstration of the NH2-terminal tryptophyl-peptides might offer new possibilities in the diagnosis of obscure human lung tumors, and it might be a valuable aid in the demonstration of the endocrinological activity in general.

Adrenocorticotropic Hormone↗
PubMed1979

Quantitative fluorescence histochemistry of combined formaldehyde-chloral-induced fluorescence of amino-terminal tryptophyl-peptide in model experiments and in the pars intermedia of the rat hypophysis.

The relationship between the intensity of combined formaldehyde-chloral vapour-induced fluorescence and the concentration of amino-terminal tryptophyl-peptide in model experiments was found to be non-linear. At a certain concentration the intensity began to increase more slowly than the concentration, and when the concentration further increased the intensity even began to decrease. Based on the studies previously reported and on the above findings it seems that fluorescence induced by combined formaldehyde-chloral vapour, glyoxylic acid vapour and possibly also other combined formaldehyde and carbonyl compounds in the hypophyseal cells containing amino-terminal tryptophyl-peptides is quenched in normal conditions due to the high local concentration. Thus, small to moderate changes in the amounts of amino-terminal tryptophyl-peptides cannot be observed by measuring the fluorescence intensity. In tissue experiments the intensity of combined formaldehyde-chloral vapour-induced fluorescence in the rat pars intermedia was measured after reserpine treatment, which decreases the number of hormone storage granules as demonstrated electron microscopically. The fluorescence intensity measurements were combined with an estimation of the amounts of amino-terminal tryptophyl-peptides extracted from hypophyses and separated in thin-layer chromatography, and subsequently demonstrated by combined formaldehyde-chloral vapour and a protein stain (amido black). Reserpine treatment decreased the fluorescence intensity in the pars intermedia and in thin-layer chromatography, and the staining of the fluorescent band with amido black was also decreased. Amino-terminal tryptophyl-peptides appeared to be depleted from the pars intermedia cells together with endorphins and other hormones of the ACTH/MSH cells containing tryptophan.

Animals↗