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Biomedical subjects

S Perry

Publications and source records attributed to S Perry.

At least 217 records · Page 12Linked to original sources
PubMed1977

Preparation of mononuclear cell subpopulations from normal human peripheral blood.

By the use of velocity sedimentation at unit gravity, in combination with methods of cell separation based on differences in density and in surface membrane receptors, it has been possible to obtain concentrates of mononuclear cell subpopulations from normal human peripheral blood. T and non-T lymphocytes have been harvested with a good yield and a high degree of purity. Monocytes may also be efficiently recovered using these procedures.

Basophils↗
PubMed1976

Oncogenesis in human acute leukemia.

By selecting, for discussion, some aspects of oncogenesis in human acute leukemia, we hope to focus attention on areas of current interest which promise to provide important advances in our knowledge and understanding of human neoplasia, and, in time, may lead us nearer to the goal of disease control and eventual eradication.

Acute Disease↗
PubMed1976

Lysosomal acid hydrolases in human lymphocyte subpopulations.

Acid phosphatase and beta-glucuronidase activities have been determined cytochemically in T, B and null lymphocytes as part of an effort to charaterize the human haemopoietic stem cell. A combination of weak acid phosphatase activity and strong beta glucuronidase staining, in the form of a single large granule, has been shown to be specific for T cells. On the basis of this approach alone, non-T cells could not be further subclassified. Further cytochemical evaluation is being explored.

Acid Phosphatase↗
PubMed1976

Lymphocyte purification from normal human peripheral blood by filtration through columns of cotton wool.

With a view to simplifying the separation of haemopoietic stem cell concentrates from human peripheral blood, in preparation for their clinical use, the production of lymphocyte concentrates by cotton wool filtration has been investigated. Using the method which is described in detail, highly purified suspensions may be prepared without the selective loss of thymus-independent lymphocytes. Optimal operating conditions are defined. Haemopoietic stem cell concentrates may thereby be harvested, and are shown to be capable of proliferation and differentiation on subsequent culture in vivo.

Cell Separation↗
PubMed1976

A reappraisal of cellular labeling with radiochromium salts.

The present studies demonstrate that, while erythrocytes preferentially bind hexavalent 51Cr, leukocytes bind the trivalent form with equal avidity. Red cells may be saturated with label in contrast to the binding of 51Cr to leukocytes, which may be described by first-order kinetics. Hexavalent label is rapidly reduced in leukocytes in contradistinction to the finding in red cells. The nonreutilization of label by erythrocytes and leukocytes is not due to inappropriate valency but to protein binding of the label. Unlike nonleukemic cells, leukemic leukocytes elute a large fraction of their label. These factors have been largely overlooked in many previous clinical studies using labeled cells, and they are of sufficient quantitative importance to compromise seriously the validity of numerous earlier reports.

Animals↗