Biomedical subjects
Publications and source records attributed to S R Pal.
Infant mice (NMRI strain) showed the inhibition of hepatic extramedullary haemopoiesis by oral inoculation of a 100 ID50 dose of EB rotavirus and nucleoprotein of SA-11 rotavirus (serotype 3). The extramedullary haemopoiesis was observed by oral inoculation of surface protein VP7 of SA-11 rotavirus and in control (placebo administered) mice.
An epidemic of encephalitis occurred in the eastern, paddy growing districts of Haryana state between July and November 1990. One hundred and eighty-two patients with encephalitis were admitted to different hospitals in Haryana and 118 of them expired (mortality rate 64.8%); 88% of the patients were children. The male to female ratio was 2. 3. 1. IgM class of antibodies to Japanese Encephalitis (JE) Virus could be demonstrated in the CSF and/or sera of 3 surviving and 2 fatal patients of the 19 patients studied. This is the first detailed report on an epidemic of encephalitis in North-Western part of India. Serologically proven cases of JE are being reported, for the first time, from this region.
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All 77 HBsAg positive and 94 of representative HBsAg negative sera derived from 253 adult patients with acute sporadic viral hepatitis during 1987 at Chandigarh were tested for serological markers of hepatitis B virus (HBV) and hepatitis A virus (HAV) infections. The HBsAg positive patients could be classified as acute hepatitis B (64%); chronic hepatitis B with exacerbation, or liver damage by other infectious or noninfectious agent/s over a chronic HBsAg carrier state (35%); and coinfection of HBV and HAV (1%). Among the HBsAg negative hepatitis group, acute hepatitis B was diagnosed in 4 per cent of patients and acute hepatitis A in another 4 per cent.
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Delta virus infections, so far, have not been documented from India. Here we report for the first time the endemicity of delta virus infection in this geographical area. HBsAg positive serum samples from 81 patients and 10 healthy voluntary blood donors were tested for delta antigen. Seven out of 49 (14.3%) sporadic acute hepatitis cases, 3 out of 8 (37.5%) chronic renal failure patients requiring frequent haemodialysis, 1 out of 22 (4.6%) chronic liver disease cases and 1 out of 10 (10%) apparently healthy voluntary blood donors had delta antigenaemia.
Acetone, carbontetrachloride, ethyl alcohol, mixture of ethyl alcohol and acetone, and heat were assessed for fixative property for direct immunofluorescent (IF) staining of antibody-coated Candida cells. The results indicated that ethyl alcohol was the most suitable fixative for the test. Antisera containing 16 units of Candida albicans type A agglutinin were found essential to get optimal detectable fluorescence of antibody-coated yeast cells. IF test showed cross reactivity between the yeasts of C. albicans and C. tropicalis. However, there was no cross reactivity with the conidia of A. flavus. The direct IF test could demonstrate antibody-coated yeast cells and pseudomycelia in deposits of urine in the direct smear. It correlated well with microscopy and culture studies. At times, it could demonstrate the antibody-coated yeasts earlier than routine significant culture. It could also differentiate the significant from non-significant fungal isolates from urine.
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Acute hemorrhagic conjunctivitis (AHC) has been reported in epidemics. This is a report of 29 sporadic cases of AHC recorded over a period of two years after the 1981 epidemic in and around Chandigarh. A rapid and simple immunofluorescence test was developed and standardized in our Institute for the diagnosis of AHC. It was used for epidemiological monitoring of cases and to establish the diagnosis in suspected cases of AHC reporting after the epidemic. The clinical picture of epidemic and sporadic cases is compared.
During 1981-1982, epidemics of acute hemorrhagic conjunctivitis (AHC) occurred in and around Chandigarh, India. Conjunctival swabs were taken from AHC cases and investigated by the indirect immunofluorescence method both in Chandigarh and in Houston. By using reference antiserum, both laboratories proved enterovirus type 70 to be the causative agent of the epidemics. The immunofluorescence test was evaluated as a rapid method for the diagnosis of AHC.
An epidemic of acute haemorrhagic conjunctivitis (AHC) occurred in and around Chandigarh, north India, during June, July and August 1981. Considering the difficulty of virus isolation, the indirect immunofluorescence test was used for the demonstration of virus-specific antigen in the cytoplasm of exfoliated conjunctival cells, using reference antisera. The epidemic appeared to be primarily due to enterovirus type 70. The method was found suitable for the rapid diagnosis of AHC cases and for detecting subclinical infection among healthy subjects and convalescent carriers. During follow-up, it was observed that some of the AHC cases were shedding virus-infected conjunctival cells for a prolonged period, even after clinical recovery and during convalescence, before they became free from virus. In the absence of virus isolation, this method can be considered as suitable for the epidemiological monitoring of AHC.
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Studies of peripheral blood lymphocytes were performed in 41 patients with acute viral hepatitis, in grade III-IV coma; 16 patients were in the third trimester of pregnancy. There were significant reductions in absolute lymphocyte count and T cell number in patients who succumbed to the disease, when compared with those who survived. B cell counts were similar in the two groups and migration inhibition test with BCG antigen was normal. It is postulated that a decrease in the number of cells interacting in cell-mediated immune reactions is related to prognosis in acute viral hepatitis.
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