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Biomedical subjects

S R Pattyn

Publications and source records attributed to S R Pattyn.

At least 145 records · Page 8Linked to original sources
PubMed1975

Replication of arboviruses in mouse organ cultures. II. Multiplication of virulent and avirulent Semliki Forest and western equine encephalitis viruses in mouse organ cultures.

The titre of WEE virus produced by organ cultures of brain, muscle and spleen decreased as tissue was taken from older mice. The multiplication of SFV and SFA in muscle organ cultures of mice remained nearly constant, whereas in spleen organ cultures, titers of both viruses decreased with increasing age of mice. There was a striking difference in the rate of decline of the yield of brain organ culture infected with virulent and avirulent SF virus strains. Organ cultures made from the brains of mice over 16 days old were totally resistant to SFA infection, whereas similar cultures from mice up to 42 days of age supported SFV multiplication. These results show that the avirulence of this strain of SF virus is due to age related intrinsic brain insusceptibility.

Age Factors↗
PubMed1975

Minimal inhibitory dosage of rifampicin in intermittent treatment of Mycobacterium leprae infection in mice.

The total minimal inhibitory dose of rifampicin determined in the experimental mouse model, was found to be 10 mg/kg body weight, administered once a week for 6 weeks or once every 2 weeks for 12 weeks. From these and other results it is suggested that administration of RMP in human treatment can be reduced to a total amount of 7.2 either as a 600 mg dose once a week for 12 weeks or as a 900 mg dose once a week for 8 weeks. At present these regimens can only be used as an introductory treatment for multibacillary cases and are still too expensive for developing countries, but their efficacy should be evaluated in the field as sole treatments in tuberculoid cases, since they could signify a substantial economy for the management of the majority of leprosy infections.

Animals↗
PubMed1973

The problem of cultivation of Mycobacterium leprae. A review with criteria for evaluating recent experimental work.

Some criteria are presented to help evaluate papers appearing in the literature claiming successful cultivation of M. leprae either in the absence or in the presence of tissue-cultured cells. Recently, electron microscopic studies have definitely shown M. leprae to belong to the genus Mycobacterium and its division to occur through transverse section. A survey is given of the mycobacterial strains isolated in the last 10 years from leprosy lesions. These strains belong to taxonomically different species and cannot be considered to be M. leprae. No substantiated claim was made concerning the in vitro growth of M. leprae and the application of the tissue culture technique has been equally disappointing. The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques.

Animals↗