BCG vaccination and tuberculosis in students of nursing.
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Biomedical subjects
Publications and source records attributed to S R ROSENTHAL.
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The present study verifies an earlier one in which it was shown that there is a direct relationship between the zinc content of the glycerol in the medium used for the preparation of BCG vaccine and the type of growth obtained. In the previous work zinc was added directly to the glycerol. In this report a more reliable and practical method is suggested, i.e., the storage of 96% reagent-grade glycerol in galvanized or zinc-lined tanks for periods of at least 5 months. Whereas poor sunken growths were obtained when the original glycerol was used, media made with glycerol stored in the zinc-lined tanks until its zinc content attained levels of from 5 to 10 p.p.m. yielded crisp, dry and abundant growths. There was no appreciable increase in the iron, copper or lead content of the stored glycerol.
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In experimental work with tuberculin testing-for example, for comparing different testing procedures, for standardizing tuberculin, etc.-two tests are often given to each person. Because of variations in allergy from person to person, such duplicate testing gives a much more precise comparison of two test procedures for a given number of persons than does the alternate use from person to person of two test procedures to be compared. In adopting this method of testing it is tacitly assumed that the two tests in the same person do not interact in any way. The present paper shows, however, that this assumption is not justifiable.The authors describe an experiment in which 611 infants who had been BCG-vaccinated at 2-5 days of age were tuberculin-tested 3-5 months later, 306 of them with a single 10 TU test and 305 with a 10 TU test in one arm and a 100 TU test in the other arm. The results showed that there was a highly significant difference between the two groups, both in the average size of the reactions and in the degree of induration, the single-test group giving the larger and stronger 10 TU reactions.
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