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S Repse

Publications and source records attributed to S Repse.

At least 19 recordsLinked to original sources
PubMed2006

Treatment results of colorectal cancer--10-years series of UMC Ljubljana (1991 - 2000).

UNLABELLED: In Slovenia the incidence of colorectal cancer is growing rapidly. In 1998 1022 new cases were registered. Our study compares results of two groups of patients with colorectal cancer. Patients and methods. In the period from 1.1.1991 to 31.12.2000 1478 patients with a colorectal carcinoma underwent potentially curative resection. We divided them in two groups, one operated in the first 5-years and second in later 5-years period. 5-years survival was estimated with Kaplan-Meier statistical analysis. Patients who died within 30 days after the operation were censored. Differences in survival curves between both groups were assessed by the log rank test. RESULTS: We resected 1478 /1599 (92,4%) patients. There was 913 (61,7%) patients resected with colon cancer and 528 (35,8%) with rectal cancer and 37 (2,5%) with sinhronius tumors. R0 resection was performed in 1174 (79,4%) patients, R1 in 29 (2,0%), and R2 in 273 (18,5%) patients. Postoperative mortality rate in resected patients was 5,48% (81/1478), in the group with paliative operations was 17,35% (21/121). Overall five-years survival rate was 54,9% (56,18% for colon cancer and 52,4% for rectal cancer Five-years survival rate for the patients with radical resection (R0) was 66,54% for colon cancer and 59,47% for rectal cancer. CONCLUSION: 5-years survival for R0-resected patients with colon cancer was in the last period from 1996 to 2000 statistically significantly better compared with the period from 1991 to 1995 (76% vs 60%) in stage I (p = 0,04048) and in stage III (p = 0,01842). 5-years survival for R0-resected patients with rectal cancer was significantly better in the same period (63% vs 55%) (p = 0,03627) in stage III (p = 0,01663).

Adult↗
PubMed2004

Mutations in the hMLH1 gene in Slovenian patients with gastric carcinoma.

Alterations of multiple oncogenes and tumor suppressor genes, together with genetic instability, are responsible for carcinogenesis in gastric cancer. The microsatellite mutator phenotype is the cause of many somatic frameshift and point mutations in non-coding repetitive sequences and in coding regions associated with cell proliferation and apoptosis. Genetic mutations in hMLH1 and transcriptional silencing of its promoter by hypermethylation lead to the inactivation of the mismatch repair system. In our study, we screened for mutations the hMLH1 gene in patients expressing the microsatellite instability genotype by using single-strand conformational polymorphism analysis and direct sequencing. Seven changes were identified; of these, three (A92P, E433Q, and K618A) were germline mutations and the other four (IVS5 453 + 79 A > G, I219V, 1039 - 7 del (T)(n), and IVS15 1668 - 19 A > G) germline polymorphisms. A92P and E433Q are novel, previously unidentified mutations. In addition, we found a rather complex distribution of mutations and polymorphisms in individual patients and in two cases also a methylated hMLH1 promoter.

Adaptor Proteins, Signal Transducing↗
PubMed2001

Assessment of differential expression of oncogenes in gastric adenocarcinoma by fluorescent multiplex RT-PCR assay.

We screened samples of tumour and peripheral normal tissue for differential expression of oncogenes by using an approach of detecting the differences in expression of a number of oncogenes simultaneously. Total RNA was isolated from 29 pairs of normal and tumour tissue samples from patients with gastric adenocarcinoma. Seven pairs of primers for oncogenes most probably associated with the process of carcinogenesis in stomach including cyclin E, c-erbB-3, HGR, c-met, TDGF/cripto, FGF-4, and EGF were used for the construction of fluorescent multiplex RT-PCR. Sense primers were 5' end-labelled with a fluorescent dye. 5-7 gastric oncogenes were simultaneously analysed for overexpression. Multiplex reverse transcription with a set of unlabeled primers was followed by a PCR reaction by adding the corresponding set of fluorescent labelled PCR-primers. Expression of oncogenes was compared to GAPDH internal standard. Multiplex fluorescent RT-PCR results were analysed by capillary electrophoresis on ABI-PRISM 310 Genetic Analyzer. Differential expression of oncogene mRNAs in tumour and normal tissue was assessed by comparison of oncogene/GAPDH ratios in tumours and their peripheral normal mucosa. Our results show, that in most patients, comparing to normal tissue, we could estimate overexpression of at least one oncogene in a sample.

Adenocarcinoma↗
PubMed1998

Adjuvant intraperitoneal chemotherapy with carbon-adsorbed mitomycin in patients with gastric cancer: results of a randomized multicenter trial of the Austrian Working Group for Surgical Oncology.

PURPOSE: Previous studies have demonstrated a beneficial effect of intraperitoneally applied mitomycin bound to activated carbon particles (M-CH) in preventing intraabdominal recurrence following curative surgery for gastric cancer. The Austrian Working Group for Stomach Cancer, a subgroup of the Austrian Working Group for Surgical Oncology, initiated a multicentric phase III trial to evaluate the safety and efficacy of this treatment regimen. PATIENTS AND METHODS: A total of 91 patients with a radically resected gastric cancer infiltrating the serosal surface were randomly assigned to receive either 50 mg mitomycin bound to a solution of 375 mg carbo adsorbens intraperitoneally before closure of the abdominal wound (n = 46) or served as a surgical control group (n = 45). Postoperative complications and recurrence-free and overall survival were evaluated to analyze the risks and benefits of this treatment. RESULTS: After a median observation period of 597 days (range, 72 to 1,096), a significantly higher postoperative complication rate was observed in the M-CH group (35%) compared with the control group (16%) (P < .02). In accordance with this finding, the postoperative (60 days) mortality rate was also significantly elevated in the M-CH group (11% v 2% in the control group). Since analysis of overall and recurrence-free survival failed to show any beneficial effect of M-CH therapy, the protocol committee decided to stop further recruitment of patients onto this study. CONCLUSION: Adjuvant intraperitoneal therapy of gastric cancer by mitomycin bound to activated carbon particles is associated with an increased rate of postoperative complications. However, no benefit for prognosis following radical resection of locally advanced tumors was observed in this multicenter phase III trial.

Adenocarcinoma↗
PubMed1997

DNA flow cytometry in gastric carcinoma: implication in patients with potentially curative resection.

BACKGROUND AND OBJECTIVES: The relevance of DNA ploidy as a prognostic factor in patients with gastric cancer is controversial. The prognostic significance of DNA ploidy and its relationship to conventional histological grading and staging of the tumor (TNM stage, Lauren, Ming and WHO classification) were evaluated. METHODS: DNA ploidy of the tumor was determined by flow cytometry on archival material from 76 patients who underwent R0, D2 stomach resection. RESULTS: DNA aneuploidy was found in 39 cases (51%). No significant association between DNA aneuploidy and either patients' sex, pT, pN, type according to Ming or Borrmann and tumor localization was found. The incidence of DNA aneuploidy was significantly lower in tumors of diffuse type according to Lauren, in signet-ring cell or undifferentiated type (WHO), in grade 3/4 tumors, and in patients younger than 50 years. We found no significant difference in survival of patients with DNA aneuploid when compared to DNA diploid tumors, although the prognosis of the patients with lower DNA index (DI < 1.2) tended to be better than that of higher DNA index (DI > 1.2). CONCLUSIONS: DNA ploidy appears to be of limited prognostic value after R0, D2 resection of stomach cancer.

DNA, Neoplasm↗
PubMed1997

Is DNA aneuploidy a prognostic factor in gastric cancer?

Flow cytometric analysis of DNA ploidy was performed on an archival material obtained from 79 patients with gastric cancer who underwent a potentially curative (R0) stomach resection with D2 lymphadenectomy, and evaluated its relationship to conventional pathologic parameters, TNM stage and prognosis. No significant association between DNA aneuploidy and either patients' sex, depth of tumor infiltration (pT), lymph node involvement (pN), histological type according to Ming, macroscopic type according to Borrmann or tumor localization was found. However, the incidence of DNA aneuploidy was significantly lower in tumors of diffuse type according to Lauren, in tumors of signet ring cell or undifferentiated type according to WHO classification, in poorly differentiated/undifferentiated tumors and in patients younger than 50 years. We found no significant difference in survival between patients with DNA aneuploid tumors and those with DNA diploid tumors. Although the prognosis of patients with tumors of lower DNA index (DI < 1.2) tended to be better than for those of higher DNA index (DI > 1.2), the difference did not reach a statistically significant level (p = 0.09). TNM stage, depth of tumor infiltration (pT) and lymph node involvement (pN) were the only factors that significantly affected survival in univariate analysis and both pT and pN retained their independent prognostic significance in multivariate analysis.

Age Factors↗
PubMed1983

[Angiodysplasia - a cause of gastrointestinal bleeding].

Angiodysplasia is a rare cause of gastrointestinal bleeding. The lesion has a specific appearance - markedly enlarged closely grouped veins with short channels between veins and arteries. The lesion may occur on any part of the gastrointestinal tract, but it is most often located in cecum. Its clinical manifestations are predominantly seen in patients beyond the age of 55 years. Selective angiography is the most certain way of diagnosing angiodysplasia. It can be diagnosed by endoscopy too. Resection of the inflicted part is the most successful treatment. Sometimes endoscopic coagulation or embolisation lisation have good results too. Three patients with angiodysplasia - a 74-year man, a 65-year woman with angiodysplasia on cecum and a 63-year woman with angiodysplasia on jejunum are shown. The diagnostic and therapeutic problems of this rare illness are discussed.

Aged↗
PubMed1982

[Hemangiomas of the liver].

Hepatic tumors are mostly due to metastasizing malignant lesions of the gastrointestinal tract. Benign neoplasms of the liver occur rarely. The most common benign tumor is the hemangioma, the etiology of which is still poorly understood. Small hemangiomas are usually asymptomatic and found incidentally at operation or post mortem examination. The treatment of large localized hemangiomas is surgical resection. When this is not performable, we have recourse to local excision of the tumor with ligation of the adjacent blood vessels. Actinotherapy is an alternative method used in treatment of multiple non localized hemangiomas. Biopsy of hemangioma is a hazardous procedure, which may trigger off massive hemorrhage. The paper presents 4 of the 92 patients subjected to liver resection during 1975 and 1980.

Hemangioma↗
PubMed1981

[Management of liver injuries].

Evidently, the most frequent cause for surgical intervention on liver is traumatic rupture of the liver, which are often irregular or multiple. The therapy is not uniform. In noncomplicated ruptures the best management is to remove necrotic tissue by digitoklasis and individual ligation of blood vessels and bile ducts with interrupted suture. In extensive lesion, where a large part of hepatic parenchyma is devitalized, the best results are achieved by resection. The mortality largely depends on well-timed operation, whereas each delay increases the surgical risk.

Humans↗