Biomedical subjects

S S Gangwal

Publications and source records attributed to S S Gangwal.

3 recordsLinked to original sources

PubMed1994

Dissolution test method for rifampicin-isoniazid fixed dose formulations.

A dissolution procedure for a rifampicin-isoniazid combination formulation was evaluated using 0.1 N hydrochloric acid solution and 0.4% (w/v) sodium lauryl sulphate solution as dissolution media. Rifampicin and isoniazid along with degradation components were chromatographed using reversed-phase liquid chromatography on a 10 microns octadecylsilica column using methanol-0.01 M disodium hydrogen phosphate (70:30, v/v; pH 4.6 +/- 0.1) as mobile phase. The detection was carried out at 254 nm. The data obtained indicate that the dissolution medium consisting of 0.4% (w/v) sodium lauryl sulphate solution is suitable for such a combination. The degradation observed in dissolution medium consisting of 0.1 N hydrochloric acid was 10-23%.

Capsules↗

PubMed1993

Determination of diltiazem hydrochloride in human serum by high-performance liquid chromatography.

A simple and sensitive reversed-phase high-performance liquid chromatographic method for the determination of diltiazem in human serum has been developed. The method involves a one-step deproteinization of serum for sample clean-up using acetonitrile. A LiChrosorb RP-8 column (30 cm x 4.1 mm I.D.) was eluted isocratically with acetonitrile-0.01 M dibasic sodium phosphate (40:60, v/v) containing 0.01% triethanolamine. Diltiazem was monitored at 237 nm and 0.1 a.u.f.s. The completion time for assay was less than 15 min, and the lower limit of quantitation was 10 ng/ml for a 100-microliters injection volume. Using this method, the pharmacokinetic parameters were calculated from a serum concentration versus time profile of diltiazem.

Acetonitriles↗

PubMed1993

Reversed-phase high-performance liquid chromatography of ketorolac and its application to bioequivalence studies in human serum.

A reversed-phase high-performance liquid chromatographic assay was used to study the bioequivalence of the anti-inflammatory drug (+/-)-ketorolac in human volunteers. Following deproteinization of human serum with 5% zinc sulphate solution, ketorolac was chromatographed on a 10-microns octadecylsilica column using acetonitrile-water as mobile phase and ultraviolet detection at 313 nm. Under these conditions the method was reproducible with a coefficient of variation of less than 5%. The assay procedure was linear in the range 0.25-1.5 micrograms/ml, with a sensitivity of 0.01 micrograms/ml ketorolac. The recovery of ketorolac from serum ranged from 90 to 95%.

Analgesics↗