Biomedical subjects

S S Grigorian

Publications and source records attributed to S S Grigorian.

At least 55 records · Page 3Linked to original sources

PubMedDate not supplied

[Natural killer activity in respiratory papillomatosis and its changes after interferon treatment].

A method to determine changes in the activity of natural killers (NK) in treatment of juvenile respiratory papillomatosis in children and correlation with the clinical course of the disease was developed and studies with whole blood specimens were carried out before interferon (IF) treatment and after it. Initially high levels of NK activity and their subsequent decline were observed in the cases where IF treatment had no effect, whereas initially low levels with subsequent increase after treatment or normal levels remaining unchanged in the course of treatment were typical of the patients manifesting some clinical effect of the treatment. These studies confirm the possibility of the modulating effect of IF, and the method may be used in mass surveys and working with children.

Child↗

PubMedDate not supplied

[The interferon status in helminthiases. 2. Alveolar hydatid disease].

In 11 patients with Echinococcus multilocularis infection the blood levels of alpha-interferon (alpha-IF) and gamma-interferon (gamma-IF) were measured using vesical stomatitis virus culture. In patients with the disease the contents of alpha- and gamma-IF were significantly (p less than 0.01) lower than in healthy controls: 392 +/- 101.6 and 101.6 +/- 20.9 U/ml and 1024 +/- 83.2 and 187 +/- 16.9 U/ml, respectively. The lower levels of alpha- and gamma-IF were found in patients with multiorgan damages including the lungs, as compared with the liver and abdomen infestation: alpha-IF 160 +/- 56.6, gamma-IF 88 +/- 24.0 and 525.7 +/- 124.3 and 109 +/- 18.3 (p less than 0.01), respectively. The similar data have been found in patients with hydatid disease (S. N. Suntsov et al., 1990). After 5 to 6 courses of mebendazole therapy in a dose of 50 mg/kg body weight dai y for 30 days with 30-day intervals there was a tendency to a slight elevation of cytokines levels (S. N. Suntsov et al., 1990), as seen in hydatid disease. The estimation of IF levels in multilocular echinococcosis infection may be of prognostic value and perhaps for the evaluation of the efficacy of surgical and chemotherapeutic treatment.

Adult↗

PubMedDate not supplied

[The antiviral activity of amiksin and its effect on the interferon status in hepatitis in mice].

A single administration of amixin followed by oral infection with mouse hepatitis virus provided protection of 40%-50% animals for 72 hours and normalized the interferon status. In parallel with decreasing level of virus-induced endogenous interferon the interferon-inhibiting activity of lymphocytes of the infected animals increased and approached normal levels.

Animals↗

PubMedDate not supplied

[Mathematical model of intracranial blood-cerebrospinal fluid dynamics system applied to the study of extreme conditions].

A mathematical model of cerebral blood and lymph circulation that can be used to study various effects (simulated space flight factors, functional loads) on the human body was developed. Biophysical patterns that determine relationships between cerebral blood and lymph circulation as well as between fluid volumes and pressures (P and V) are discussed. Involvement of the mechanism of brain circulation autoregulation in cerebral blood and lymph circulation is considered. The model was applied to study responses of cerebral blood and lymph circulation to various exposures such as orthostatic and antiorthostatic tests and provocative tests which led to intracranial pressure shifts and modified the ratio between volumes and pressures in different hydrodynamic systems. The modelling data were consistent with clinical, physiological and experimental observations which demonstrate an adequacy of the above model.

Aerospace Medicine↗

PubMedDate not supplied

[Interferon induction via peroral administration of high-molecular polynucleotides].

High molecular polynucleotides, poly(I).poly(C) and poly(G).poly(C), incorporated into liposomes may be used for serum interferon induction when administered orally. Titres of interferon induced by this method are sufficiently high (320-640 units/ml) and close to those induced by the same preparations administered parenterally (640-1280 units/ml). The use of liposomal polynucleotides results in prolonged (up to 24 hours) circulation of interferon in the blood of mice at a sufficiently high level. Liposomal polynucleotides are low-toxic upon oral intake. The liposome-polynucleotide complex is sufficiently stable on storage, and retains the interferon-inducing activity, if given orally, after 6 months of storage at 4 degrees C in the liquid form.

Administration, Oral↗

PubMedDate not supplied

[Vasodilators as interferon inducers].

Vasodilating drugs, inhibitors of cAMP phosphodiesterase activity (theophylline, caffeine, theobromine, etc.) and other vascular drugs (papaverin, dibasol, no-spa, etc.) were found in experiments in vivo to be capable of inducing interferon the peak of whose production was determined in the blood of animals 24 hours after drug administration (the observation period). The interferon production is critically dependent upon the dose and routes of administration of the drugs. The interferon induced by vasodilating drugs belongs to interferon of the 1st species. It is assumed that both endothelial cells of vessel walls and lymphocytes may take part in interferon production.

Administration, Oral↗

PubMedDate not supplied

[Interferon-inducing activity of liposome-incorporated double-stranded polynucleotides and the means for its enhancement].

Natural double-stranded RNA (dsRNA) incorporated into liposomes upon parenteral inoculation induces 4 times as much amounts of interferon as inoculation of the equal amount of dsRNA without liposomes. Oral administration of liposome-incorporated dsRNA induces in animals serum interferon in amounts similar to those induced by parenteral inoculation of dsRNA without liposomes (320-640 units/ml). When liposome-incorporated dsRNA is used, interferon induction is prolonged to 24 hours. The prolongation period increases to 5 days after preliminary treatment of animals with "empty" liposomes. In M-19 cell culture, 2-hour treatment with liposome-incorporated dsRNA in a dose of 5-10 micrograms/ml induces a yield of 640-1280 units/ml interferon and 100% antiviral effect. The L-929 culture is more sensitive to dsRNA in liposomes. Even its minimal amounts (0.1-1 microgram/ml) after 2-3-hour contact produce a 100% antiviral effect in the presence of low amounts of interferon in the culture fluid (20-40 units/ml) or in its complete absence.

Animals↗

PubMedDate not supplied

[Characteristics of interferon production from the enteral administration of inducers].

When interferon inducers are administered to animals, autonomous local production of interferon by organs (intestine, liver) occurs alongside with the production of serum interferon. After oral administration of a low molecular interferon inducer, tilorone, the intestine is the main site of interferon synthesis. Here the peak of interferon production precedes that in the serum by 20 hours and exceeds it 16-fold. The sequence of events follows the scheme: intestine--liver--serum. Upon oral administration of high molecular inducers incorporated into liposomes (polyguacyl, lafarin) the predominant site of interferon production is the liver where over 80% of the administered inducer is localized and interferon production is 2-4-fold higher than in the intestine. The sequence of events follows the scheme: liver--intestine--serum.

Administration, Oral↗

PubMedDate not supplied

[Antiviral activity of amiksin incorporated into liposomes].

The results of a comparative study of the antiviral activity of amyxin (tilorone) without liposomes and incorporated into liposomes on the model of experimental enteral hepatitis of mice are presented. The use of amyxin in liposomes was shown to prolong interferon production and to provide significant 60% protection of the animals. The possible mechanism of increasing the antiviral effectiveness of amyxin upon its incorporation into liposomes, enteral administration and subsequent enteral infection of mice with hepatitis virus is discussed. It is assumed that use of amyxin in liposomes may increase its effectiveness in prevention of human hepatitis A.

Animals↗

PubMedDate not supplied

[Evaluation of the interferon status of human subjects by whole-blood specimens].

A simple method for the determination of three parameters of human interferon status: serum interferon (IFN) and induction of alpha- and gamma-IFN in specimens of 10-fold-diluted whole blood in a volume of 200 microliters is described. Standard parameters of interferon status of normal subjects in the whole blood are given which can be visually presented as a triangle of interferon status, and its deviations in various pathological conditions. The whole-blood method is recommended for large-scale clinical surveys, especially in pediatrics.

Biological Assay↗

PubMedDate not supplied

[Disordered interferon status in patients with urticaria and angioneurotic edema against a background of frequent ARVI: its correction with reaferon].

The results of clinical, allergological, virological, and immunological examinations of patients with urticaria and angioneurotic edema against the background of frequent ARVI are presented. Disorders in the interferon system in these patients were studied. A positive anti-recurrent effect of prophylactic parenteral course of a national recombinant alpha 2-interferon (reaferon) in this pathology has been demonstrated.

Acute Disease↗

PubMedDate not supplied