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Biomedical subjects

S Shioda

Publications and source records attributed to S Shioda.

At least 163 records · Page 9Linked to original sources
PubMed1989

Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas.

Variant sublines LMF3, LMF4 and LMF5 with high metastatic potential were established from a human oral squamous carcinoma cell line HSC-3. These sublines metastasized to the draining lymph nodes after subcutaneous inoculation into nude mice. They were obtained by sequential selection in vivo from the parent HSC-3. At each step, the cells which metastasized to lymph nodes were cultured and reinoculated into nude mice. Two other cell lines HSC-2 and HSC-4 were also established from other patients, that had neither invasive nor metastatic potential. Biologic properties were compared among high metastatic, low metastatic and non-metastatic cells. Metastatic cells grew rapidly and invaded into surrounding tissues at the inoculated site. The incidence of pulmonary colonization after intravenous injection of tumor cells was high in selected variants. Metastatic cells formed diffuse colonies in type I collagen matrix and had a higher tendency to adhere to type IV collagen network.

Animals↗
PubMed1989

[Reconsideration of the bilaminar zone in the retrodiscal connective tissue of the TMJ. 1. Relation between discal fiber and the condyle and components around the disk].

The fibrous connection of the discal attachment to the condyle and to the other structures around the disk was investigated histologically in order to reconfirm what Rees calls the bilaminar zone. Eleven TMJs were taken from fresh cadavers. Sections, obtained from six materials with condyle protruded, were observed. The Results were as follows: Most of the collagen fibers composing the articular disk attached to the area from the medial and lateral pole to the corner region of the posterior aspect of the condyle. But these fibers were not recognized on the posterior aspect of the condyle. A few of the fiber bundles composing the supra-lateral part of the disk were linked with the fibers of the periosteum of the lateral end of the articular tubercle. The fibers of the superficial layer of the posterior band linked with the fibers beneath the synovial membrane of the retrodiscal area, but these fibers were not observed to connect the disk with the posterior wall of the fossa. At the medial end of the disk, the elastin rich fibers originating from the petrotympanic fissure attached to the disk. There were no other fibrous structures directly connecting the disk with the posterior wall of the fossa.

Cartilage, Articular↗
PubMed1989

[Reconsideration of the bilaminar zone in the retrodiscal connective tissue of the TMJ. 2. Fibrous structure of the retrodiscal connective tissue and relation between those fibers and the disk].

The fibrous structure of the retrodiscal connective tissue was investigated histologically in order to reconfirm what Rees calls the bilaminar zone. Eleven TMJs were taken from the fresh cadavers. The sections, obtained from six materials with condyle protruded, were observed mainly. The Results were as follows: The retrodiscal area was composed of the collagen fibers originating into this area from the petrotympanic fissure, from the conjunctive area of the posterior wall of the fossa with the lateral wall of the articular cavity, and from the conjunctive area of the posterior slope of the auricular tubercle with the lateral wall of the articular cavity. In the superficial layer of the retrodiscal area, these fibers crossed each other, and a few fibers jointed with these fibers from the superficial layer of the disk and from the posterior wall of the articular fossa. The arrangement of these fibers had various directions, however most of fibers tended to run medio-laterally. The fiber bundles containing many elastic fibers ran into the retrodiscal area from the petrotympanic fissure. Although these fibers connected the most medial side of the disk with the medial side of the posterior wall of the articular fossa, they had many branches composing the retrodiscal area as stayed above. The fibers were very wavy, in spite of the protruded position of the disk. So these fibers were not considered to have a role in pulling back the disk in the retruded phase of the mouth closing. New findings as to the retrodiscal connective tissue were obtain in this research. They were different from Ree's description.

Cartilage, Articular↗
PubMed1989

[Clinical application of arthroscopy on temporomandibular joint].

We present the anatomy, methodology, procedure for arthroscopy on temporomandibular joint. Arthroscopic view of normal and pathologic view on superior articular cavity of temporomandibular joint are presented. There were no serious complications except slight swelling on temporomandibular joint.

Arthroscopy↗
PubMed1989

[Arthroscopic surgery for habitual dislocation of the temporomandibular joint].

This study is to evaluate the effect of the arthroscopic surgery for habitual dislocation of the temporomandibular joint. Conclusively, it is believed that arthroscopic surgery for habitual dislocation of the temporomandibular joint is easier method, has fewer complications, and lower recurrence rate, when compared with the conventional temporomandibular joint surgery.

Arthroscopy↗
PubMed1989

A case report of papillary adenocarcinoma in sublingual region--ultrastructural and histochemical study.

A rare case of papillary adenocarcinoma located in the sublingual region is presented. This case was initially considered to be a hemangioma and then the tentative clinical diagnosis was thyroglossal duct cyst at the time of the operation under general anesthesia. However, the final histopathologic diagnosis was papillary adenocarcinoma. We investigated its origin by histological, ultrastructural and histochemical techniques and it was suggested that this tumor arose from the minor salivary gland.

Adenocarcinoma, Papillary↗
PubMed1989

Ultrasonic evaluation of cervical lymph node metastasis of squamous cell carcinoma in oral cavity.

Forty-four patients with oral cancer were examined by ultrasonography to detect cervical lymph nodal metastases. The lymph nodes, which were histopathologically or clinically confirmed to have cancer metastases, showed the following characteristic echogram findings: distinctive nodal boundary, posterior echo enhancement and an increased ratio of the shortest and the longest diameters of the node. It is suggested that these three peculiar ultrasonographic findings are considered to be important items for the differential diagnosis of benign nodes from the cervical metastasis of oral cancer.

Carcinoma, Squamous Cell↗
PubMed1988

Thyrotropin-releasing hormone-like immunoreactive neurons in rabbit medulla oblongata.

Thyrotropin-releasing hormone-like immunoreactive (TRH-LI) neuronal cell bodies and processes were identified by using the peroxidase-antiperoxidase method in the medullar oblongata of rabbits. TRH-LI cell bodies were mainly distributed in the ventral medulla (paraolivary and parapyramidal regions), and caudal raphe nuclei (nucleus raphe obscurus and nucleus raphe pallidus). TRH-LI processes with varicosities were densely distributed in the solitary nucleus, dorsal motor nucleus of the vagus nerve and area postrema. TRH-LI processes appeared to be in contact with unlabelled cell bodies in the dorsal motor nucleus of the vagus nerve.

Animals↗
PubMed1988

Electron microscopic studies of primary culture cells from ameloblastoma.

Primary culture cells from eight cases of ameloblastoma were obtained and observed by means of light microscopy. Cells in four cases were observed by means of electron microscopy. Cultured cells in all cases resembled epithelial cells with the cells from follicular ameloblastoma more tightly packed than those from the plexiform types. Ultrastructurally, cells from follicular ameloblastoma contained many lysosomes, whereas cells from the plexiform type contained many vacuoles.

Ameloblastoma↗
PubMed1988

[Results of surgical treatment of tongue cancer].

Fourty-four patients with squamous cell carcinoma of the tongue have been treated by surgery. Twenty-two out of 44 patients were treated by a glossectomy alone and the remaining 22 by en bloc dissection with a glossectomy and neck dissection. The 5-year cumulative survival rate of these 44 cases was 86.1%, broken down as follows: stage I (20 cases), 100%; stage II (9 cases), 88.9%; stage III (11 cases), 72.7%; and, stage IV (4 cases), 50%. Four cases had a recurrence in the primary site, and 6 in the cervical region. Five out of 44 cases died (4 from tongue cancer, and one from other causes). Further, 3 out of 5 cases died of a recurrent cancer in the primary focus or in the neck. Thus, it has been concluded that control of the primary focus and neck metastasis is important for the treatment of tongue cancer, and that surgery also is an excellent therapy for tongue cancer.

Carcinoma, Squamous Cell↗
PubMed1988

Response of the mouse femoral muscle to an implant of a composite of bone morphogenetic protein and plaster of Paris.

A composite consisting of 1 mg of bone morphogenetic protein (BMP) and 5 mg of plaster of Paris (PLP) was implanted into the mouse femoral muscle. Control PLP without BMP was implanted into the contralateral muscle. The BMP/PLP composite induced cartilage formation within two weeks, trabecular bone within three weeks, and lamellar bone including bone marrow within six weeks. PLP did not disturb BMP-induced bone formation. In addition, the BMP/PLP composite was resorbed early after implantation. These results suggest that PLP composite system is one of the most suitable BMP delivery systems currently available.

Animals↗
PubMed1987

Innervation of thyrotropin-releasing hormone-containing neurons in the bullfrog pars intermedia.

We studied immunocytochemical localization of thyrotropin-releasing (TRH) in the bullfrog pats intermedia. Many TRH-like immunoreactive terminals containing immunoreactive granular vesicles 80-150 nm in diameter (116 nm mean diameter) were found in the pars intermedia. Some TRH-like immunoreactive terminals were in synaptic contact with the intermedia cells. These findings support the hypothesis that TRH directly activates the intermedia cells via synapses to secrete alpha-melanocyte-stimulating hormone.

Animals↗
PubMed1987

TRH axon terminals in synapsis with GRF neurons in the arcuate nucleus of the rat hypothalamus as revealed by double labeling immunocytochemistry.

Synaptic input to neurons containing growth hormone-releasing factor (GRF) by axon terminals containing thyrotropin-releasing hormone (TRH) in the arcuate nucleus (AN) of the rat hypothalamus was examined using a method combining pre-embedding peroxidase-anti-peroxidase for GRF with postembedding immunocolloidal gold staining for TRH. The TRH-like immunoreactive axon terminals were found to make synaptic contact with GRF-like immunoreactive neurons with unlabeled axon terminals. From these findings, TRH-containing neurons in the hypothalamic AN of the rat may be considered to innervate GRF neurons, to regulate GRF secretion or to have some other functions via synapses.

Animals↗
PubMed1987

Catecholaminergic innervation of neurons containing corticotropin-releasing factor in the paraventricular nucleus of the rat hypothalamus.

Catecholaminergic synaptic input to neurons containing corticotropin-releasing factor (CRF) in the parvocellular portion of the paraventricular nucleus (PVN) in the rat hypothalamus was observed. The experimental techniques used combine autoradiography after 3H-noradrenaline (3H-NA) injection or uptake of 5-hydroxydopamine (5-OHDA) with immunocytochemistry using CRF antiserum. CRF-like immunoreactive cell bodies and fibers in the PVN received synaptic inputs from the axon terminals in which a selective accumulation of 3H-NA or 5-OHDA was found. This finding suggests that the secretion of CRF neurons may be regulated via synapses by catecholaminergic neurons.

Animals↗