PubMed Health⌕ Search

Biomedical subjects

S V Kevy

Publications and source records attributed to S V Kevy.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

A protocol for cryoprecipitate production.

A three-year contract research program to study the production of cryoprecipitate has been completed. The investigators have collaborated to propose changes in the methods of production of cryoprecipitate. The investigators believe that the problem of low and variable yield of cryoprecipitate can be overcome and cryoprecipitate can be produced which contains, on an average, at least 100 Factor VIII units in nearly every bag. It is emphasized that breaches of technique and time delays in cryoprecipitate processing must be scrupulously avoided to achieve optimum, standardized yields.

Blood Donors↗
PubMedDate not supplied

The quality of over- and undercollected blood for transfusion.

Whole blood was collected in increments varying from 100 g to 600 g into plastic containers intended for 450 ml collections. In order to determine the clinical usefulness of over and undercollected bloods, red blood cell survival studies were performed after 21 and 28 days of storage and in vitro measurements were performed weekly throughout the storage period. The red blood cell survival of 21-day-old CPD bloods was acceptable if as little as 300 g of blood were collected whereas a minimum of 400 g of blood was necessary to provide normal survival after 21 days storage in ACD solution. If as much as 600 g of whole blood were collected into a standard ACD or CPD blood pack, the red blood cell survival was within normal limits after 21 days of storage. These data demonstrate the limits to which over- and undercollected units can be utilized in transfusion therapy.

Blood Banks↗
PubMedDate not supplied

Rhesus monkey platelet kinetics and storage.

Rhesus monkey platelets that are separated, 51Cr labeled, and transfused provide yields, 1/2 time and survivals similar to those obtained in man. Rhesus platelets prepared and stored at room temperature for three days have 93 per cent of the yield of fresh platelets. When prepared and stored at 4C, the yield is only 54 per cent of fresh platelets. Rhesus repeatedly transfused with platelets from random unmatched donors always have a significant decrease in the platelet 1/2 time and survival. This was observed after the second homologous transfusion in one and after the third in others. The rhesus provides an excellent platelet model with thrombocyte responses mimicking those of man.

Animals↗
PubMedDate not supplied

An in vitro evaluation of a new plasticizer for polyvinylchloride medical devices.

The rate of Hatcol-200 (H-200) leaching from polyvinylchloride (PVC) by serum is one-hundredth that of di-2-ethylhexyl phthalate (DEHP) as determined by radioassay. There is a proportional decrease in leachability of DEHP but not of H-200 as the plasma proteins are diluted. Saline extracts of DEHP demonstrated a low but progressive rate of leaching with an accumulation of 11 microgram/ml after seven weeks whereas H-200 showed only a constant residue of 0.35 microgram/ml. Normal human serum incubated for three weeks at 37 C with PVC strips plasticized with DEHP produced significant growth-inhibition of human diploid fibroblasts. Human serum incubated in an identical manner with PVC plasticized with H-200 for up to four weeks demonstrated no effect on tissue culture. These studies indicate that H-200 deserves further evaluation as a replacement for DEHP in PVC biomedical devices.

Diethylhexyl Phthalate↗
PubMedDate not supplied

The effects of irradiation on blood components.

The functional properties of formed elements of whole blood were studied following irradiation doses of 500 to 20,000 rads. Irradiated lymphocytes retained only 1.5 per cent of their 3H thymidine uptake after a 5,000-rad exposure and none after 7,500 rads. Red blood cells stored for 21 days and then irradiated with 5,000 rads had the same survival as nonirradiated controls. In contrast, 5,000 rads reduced platelet yields. However, transfused irradiated platelets produced the expected increases in platelet counts and controlled hemostasis in thrombocytopenic patients. After 5,000 rads, granulocytes had normal bacterial killing capacity, chemotactic mobility, and normal superoxide production after high-dose stimulation. Nitroblue tetrazolium reduction and ingestion stimulated by complement opsonized oil droplets were not diminished by 5,000- and 10,000-rad irradiation. The functional qualities of cellular blood components other than lymphocytes are not compromised by 5,000 rads. This irradiation dose may be an effective means of controlling incidence of graft-vs-host disease in immunosuppressed patients.

Blood Cells↗
PubMedDate not supplied

Blood component therapy during the neonatal period: a national survey of red cell transfusion practice, 1985.

A questionnaire to determine patterns of neonatal red cell transfusion practice during 1985 was mailed to 2200 blood banks of American Association of Blood Banks (AABB) institutional members and children's hospitals. There were 915 responses (41.6%); 785 responses (86%) contained sufficient data for analysis. The majority (70.6%) of 785 responding hospitals were community/urban institutions. However, more highly specialized, pediatric hospitals were also represented by 92 university/tertiary-care hospitals (11.7% of respondents) and 29 children's hospitals (3.7% of respondents). Two-thirds of hospitals performed a major antiglobulin crossmatch (rather than an abbreviated one) before all neonatal red cell transfusions. The red cell preparation most frequently selected for small-volume transfusions was ABO and Rh group-specific red cell concentrates. When performing only large-volume exchange transfusions, 19.2 percent of hospitals used whole blood; all others prepared reconstituted units of red cells plus fresh-frozen plasma, a practice that frequently causes exposure to two donors per unit. Another practice likely leading to multiple donor exposure is the use of fresh-frozen plasma to adjust the hematocrit of red cell preparations to a predetermined value prior to a small-volume transfusion. Over one-half of hospitals adjusting hematocrits used plasma, presumably from one donor, to dilute packed red cells from another donor, a practice that has no apparent medical benefit. Most hospitals (63.4%) provided red cells with a reduced risk of transmitting cytomegalovirus; blood from seronegative donors was selected by 65 percent of hospitals. The majority of hospitals, including most of the community/urban hospitals, did not irradiate blood products before transfusion.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Transfusion↗