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S V Reeh

Publications and source records attributed to S V Reeh.

2 recordsLinked to original sources
PubMed1976

A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.

A specialized transducing bacteriophage lambdadpolCdap D-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and has been verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. Evidence is presented which suggest that the structural genes for ribosomal protein S2 (rpsB) and RNA polymerase sigma factor (sit) also lies on lambdadpolCdap D-9.

Coliphages↗
PubMed1976

Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophages lambdadrifd 18 and lambdadfus-3.

The presence of EF-Tu, RNA polymerase subunit alpha, and EF-G on the lambdadfus-3 genome and EF-Tu, ribosomal proteins L7/L12, and RNA polymerase subunit beta on the lambdadrifd 18 genome has been confirmed using a two-dimensional gel electrophoresis technique sensitive to changes in isoelectric point and molecular weight. In this system two EF-Tu gene products could not be resolved. Following infection of ultraviolet light-irradiated Escherichia coli with either lambdadfus-3 or lambdadrifd18, the EF-Tu gene, tufA, near 65 minutes on the genetic map is expressed as 3-4 copies per EF-G molecule. The EF-Tu gene, tufB, near 79 minutes on the genetic map, is expressed at about one-third of this rate. alpha is expressed as 1 copy per EF-G molecule, beta as 0.14 per EF-G molecule and L7/L12 as 2.5 per EF-G. These figures compare well with the relative amounts found in exponentially-growing cells, in which the ratio of EF-Tu to EF-G is approximately 5. Almost 90% of the total number of proteins (calculated on a molecular weight basis) which theoretically can be encoded on the lambdadrifd18 have been identified on the two-dimensional gel.

Bacterial Proteins↗