A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.

J D Friesen; J Parker; R J Watson; D Bendiak; S V Reeh; S Pedersen; N P Fiil

doi:10.1007/bf00268549

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A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.

J D Friesen·J Parker·R J Watson·D Bendiak·S V Reeh·S Pedersen·N P Fiil

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Abstract

A specialized transducing bacteriophage lambdadpolCdap D-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and has been verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. Evidence is presented which suggest that the structural genes for ribosomal protein S2 (rpsB) and RNA polymerase sigma factor (sit) also lies on lambdadpolCdap D-9.

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Coliphages DNA Viruses DNA-Directed RNA Polymerases Electrophoresis Escherichia coli Genes Peptide Elongation Factors RNA, Bacterial Ribosomal Proteins Transduction, Genetic Ultraviolet Rays Viral Proteins Journal Article

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J D Friesen, J Parker, R J Watson, D Bendiak, S V Reeh, S Pedersen, N P Fiil. 1976-10-18. A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.. https://doi.org/10.1007/bf00268549

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