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Biomedical subjects

S Yoshimura

Publications and source records attributed to S Yoshimura.

At least 271 records · Page 15Linked to original sources
PubMed1984

[A retrospective evaluation of suprapubic prostatectomy].

Suprapublic prostatectomies performed on 89 patients from January 1977 to December 1981 were retrospectively evaluated as regard to operative risk, preoperative and postoperative complications. The patients were from 56 to 87 years old with a peak distribution in the seventies. Common preoperative complications were urinary tract infection, coronary sclerosis followed by hypertension. The operation time for resection and weight of resected adenoma averaged 46.5 minutes and 35.1 g, respectively. Average blood loss during operation was 179 ml. The most frequent postoperative complication was acute epididymitis. There were no operative deaths. Prostatic incidental carcinoma was found in 3 cases of resected adenomas by histological examination. There were statistically significant correlations between adenoma weight and duration of postoperative urinary tract infection (r = 0.38, p less than 0.01) and between operation time and blood loss (r = 0.42, p less than 0.01). In contrast, preoperative urinary tract infection had no influenced on duration of postoperative urinary tract infection or postoperative gross hematuria.

Acute Disease↗
PubMed1984

[Continuous measurement of cardiac output during ergometer exercise using an ultrasonic pulsed Doppler flowmeter].

A method for determining cardiac output was developed, which provided the product of the absolute blood flow rate at the aortic arch (as measured non-invasively from the suprasternal notch by an ultrasonic pulsed Doppler flowmeter: UPDF) and the cross-sectional area of the aorta at that level, as estimated from two-dimensional echograms. Using this method, non-invasive continuous measurements of cardiac output were made during bicycle ergometer exercise. The results were summarized as follows: Resting cardiac output values obtained by this method correlated reasonably well with those by the thermodilution method (correlation coefficient r = 0.76). Eight healthy male volunteers exercised for 2 min on a bicycle ergometer at 250 kpm/min, and serial cardiac outputs were determined by this method for five of these subjects during and after the loading. The cardiac output curves thus obtained clearly demonstrated abrupt increases in cardiac outputs initially, followed by rapid returns to resting cardiac output levels after the end of exercise. We intend to conduct additional studies for larger groups of subjects to further evaluate and to establish this method for use in cardiac examinations.

Adult↗
PubMed1983

Subcellular location of the major protein antigens of paramyxoviruses revealed by immunoperoxidase cytochemistry.

The major structural proteins of Newcastle disease virus and Sendai virus were localized in infected BHK-21 and MDBK cells by ultrastructural immunoperoxidase cytochemistry using antibodies against the individual viral protein antigens. The intracellular glycoproteins were strictly membrane bound, being localized in the rough endoplasmic reticulum (RER), perinuclear spaces, smooth membrane vesicles, and presumed Golgi apparatus. The nucleocapsid proteins were detected exclusively in membrane free cytosol and accumulated there, forming inclusions. The membrane (M) protein was found both in cytosol and on RER. The viral proteins on RER exhibited a distinct site specificity; the glycoproteins were facing the lumen of RER whereas M protein was present at the outer cytoplasmic surface. All the viral proteins were detectable at the plasma membrane where virus assembly takes place. However, their modes of distribution differed remarkably. The glycoproteins were spread widely over the entire cell surface including the areas of virus budding and those of normal morphology, whereas M protein was localized in restricted areas of the membrane, frequently forming a patch of virus specific membrane. The presence of nucleocapsids was confined to the virus particles budding from the plasma membrane. These results complement and extend the earlier morphological and biochemical data on the assembly or morphogenesis of paramyxoviruses.

Animals↗
PubMed1983

Non-invasive quantitative evaluation of aortic regurgitation using an ultrasonic pulsed Doppler flowmeters.

The severity of aortic regurgitation (AR) is usually evaluated using cineaortography, but this procedure cannot be carried out easily because of its invasive nature. For estimating the severity of AR non-invasively as well as quantitatively, we measured the blood flow in the aortic arch using an ultrasonic pulsed Doppler flowmeter (UPDF) from a suprasternal notch. The rugurgitant ratio was calculated from the waves of relative flow volume and compared with the severity determined cineaortographically. The following results were obtained: 1) Reproducible waves of the aortic arch flow were recorded in all normal subjects and also in 19 out of 23 patients with AR. 2) Distinctive waves of the regurgitant flow, which could not be seen in normal subjects, were recorded in AR except for some mild cases. 3) The regurgitant ratio obtained from the UPDF corresponded well with the severity based on the cineaortogram. It may be concluded that the UPDF is clinically useful and reliable for quantifying AR non-invasively.

Adult↗
PubMed1983

[Response of subunits of human pituitary gonadotropins to LH-RH 2 step stimulations in ovarian dysfunction].

To investigate the alteration of pituitary gonadotropin subunits in ovarian dysfunction by two step administration of LH-RH at 60 minutes interval, specific and sensitive radioimmunoassays of the subunits were utilized for this study. Blood samples were obtained from thirteen women of ovarian dysfunction and from fifteen normal women of subunits. The definition of delta 1 and delta 2 was as follows. delta 1(hormone release) = 30 (or 60) min value-0 min value. delta 2(hormone synthesis and release) = 90 (or 120, 180)-60 min value. delta 1 delta 2 ratio = delta 2/delta 1. Correlation was observed between the value of native gonadotropic hormones and the value of their subunits (LH-alpha and LH-beta) in normal women but no correlation was detected in delta 1 and delta 2 values in ovarian dysfunction. Thus LH-RH 2 step test suggest the possibility to differentiated pituitary function in both normal and abnormal subjects. The analysis of gonadotropin subunits was useful to develop new diagnostic criteria of ovarian dysfunction.

Adult↗
PubMed1983

[Angiomyolipoma: a case report and a statistical study of 147 cases in Japanese literature].

A 32-year-old housewife with gross hematuria and right flank pain had excretory urography and angiography performed. A large right renal mass with neovascularity was demonstrated. Computerized tomography also revealed a large well-demarcated renal mass with low value of HU. Right nephrectomy was done and histopathological diagnosis was angiomyolipoma weighing 1100 g. A statistical study was made on 147 cases of renal angiomyolipoma in the Japanese literature including this case. The male to female ratio was 1 to 2.9, and the average age of male and female patients was 37.8 and 39.0 years, respectively. Thirty-eight per cent of the cases were associated with tuberous sclerosis. The main clinical signs were flank pain, flank mass and hematuria. In 80% of the cases, nephrectomy was done because of the difficulty of preoperative differential diagnosis from renal cell carcinoma. Recently, CT and sonography have become a great help in diagnosing angiomyolipoma, because they can demonstrate the fat in the renal mass. Conservative or surgical treatment to save the kidney can be used more often when it becomes possible to make a clear differential diagnosis between angiomyolipoma and other malignant diseases.

Adenocarcinoma↗
PubMed1982

Rapid and highly sensitive method for determination of methamphetamine and amphetamine in urine by electron-capture gas chromatography.

A rapid and sensitive method for determination of methamphetamine and amphetamine in urine was developed by using electron-capture gas chromatography. The extraction procedure, the experimental conditions for pentafluorobenzoyl derivative formation and the percentage recovery of the drugs from urine are described. The pentafluorobenzoyl derivative of methamphetamine showed a higher electron-capture sensitivity and was detected in at least 23-fold lower concentration than the heptafluorobutyryl derivative which is commonly used as a derivatizing agent for the amine. The detection limit of pentafluorobenzoyl derivatives of methamphetamine and amphetamine was ca. 10 pg. A concentration as low as 10 ng/ml of methamphetamine and amphetamine in urine was easily detected by this method.

Amphetamine↗
PubMed1982

Surgical anatomy of the nasal cavity and paranasal sinuses.

The knowledge acquired by the following method can be useful not only comparison with computerized tomographs which are frequently used in otorhinolaryngology, but also will make a highly effective contribution to surgical anatomy and the prevention of complications in surgery of the nose and paranasal sinuses. The sphenoid sinus and the adjacent tissues were studied on specimens removed en bloc from 12 cadavers. Horizontal sections were made and were macroscopically and microscopically observed for surgical and anatomical studies. The optic nerve was frequently in contact with the lateral wall of the paranasal sinus which was paper-thin in sinuses with marked pneumatization. In some sinuses with marked pneumatization, the course of the internal carotid artery was in contact with the posterior wall of the sphenoid sinus. The clinical anatomy should be kept in mind to avoid complications during operative procedures. Knowledge of clinical anatomy is also useful in understanding the images of CT scanning of the paranasal sinuses.

Dissection↗
PubMed1982

Ultrastructural localization of prolactin in the rat anterior pituitary glands by preembedding peroxidase-labeled antibody method: observations in normal, castrated, or estrogen-stimulated specimen.

Ultrastructural localization of prolactin (PRL) was studied immunocytochemically (preembedding peroxidase-labeled antibody method) in a variety of pituitaries, including those from 1) normal, 2) castrated, and 3) castrated and estrogen-stimulated rats. In the normal rat, PRL was observed in cisternae of the rough endoplasmic reticulum (RER), perinuclear spaces, Golgi saccules, and secretory granules. In the castrated rats, PRL cells were rather atrophic and were filled with many small PRL-positive secretory granules. RER and Golgi saccules were rather inconspicuous and were almost devoid of PRL localization. The serum PRL level was markedly lowered. With estrogen stimulation after castration, the serum PRL level was markedly elevated and PRL cells showed a pronounced increase of PRL filled cisternae in the RER. From these observations, the role of secretory granules, Golgi apparatus, and RER in hormonal secretion was defined, and it was postulated that some peptide hormones would be secreted along two alternative pathways, i.e., either 1) a long (regulated) pathway or 2) a short (accelerated) pathway, in accordance with their secretory activities, which could be altered by various stimulations such as the use of estrogen.

Animals↗