Biomedical subjects

Sally L Gewalt

Publications and source records attributed to Sally L Gewalt.

4 recordsLinked to original sources

PubMed2002

Magnetic resonance histology for morphologic phenotyping.

Magnetic resonance histology (MRH) images of the whole mouse have been acquired at 100-micron isotropic resolution at 2.0 T with image arrays of 256 x 256 x 1024. Higher resolution (50 x 50 x 50 microns) of limited volumes has been acquired at 7.1T with image arrays of 512 x 512 x 512. Even higher resolution images (20 x 20 x 20 microns) of isolated organs have been acquired at 9.4 T. The volume resolution represents an increase of 625000 x over conventional clinical MRI. The technological basis is summarized that will allow basic scientists to begin using MRH as a routine method for morphologcic phenotyping of the mouse. MRH promises four unique attributes over conventional histology: 1). MRH is non-destructive; 2). MRH exploits the unique contrast mechanisms that have made MRI so successful clinically; 3). MRH is 3-dimensional; and 4). the data are inherently digital. We demonstrate the utility in morphologic phenotyping a whole C57BL/6J mouse.

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PubMed2002

Quantitative anatomy of the guinea pig endolymphatic sac.

The endolymphatic sac is believed to represent one of the primary loci for endolymph volume regulation in the inner ear. Quantitative analysis of physiologic measurements from the endolymphatic sac requires knowledge of the anatomy of the structure, specifically the luminal volume and the variation of cross-sectional area with distance along the sac. Recently techniques have become available to make these measurements. In the present study, fixed, isolated specimens of the guinea pig endolymphatic sac were imaged by high-resolution magnetic resonance microscopy (MRM) or by histological serial sections. Structures were reconstructed and quantified using image analysis software. In specimens imaged by MRM the endolymphatic sac volume, including tissue and lumen, was 359 nl for the intraosseous region and 106 nl for the extraosseous region, totaling 465 nl for the entire structure. The luminal volumes were 131 nl for the intraosseous region and 13 nl for the extraosseous region, totaling 144 nl. In histological specimens the volume, including tissue and lumen, was 414 nl for the intraosseous region and 121 nl for the extraosseous region, totaling 535 nl for the entire structure. The luminal volumes were 152 nl for the intraosseous region and 26 nl for the extraosseous region, totaling 179 nl. Differences in volume estimates obtained by the two methods were not statistically significant and variation was dominated by inter-specimen variation. Pooling the data, the total volume of the endolymphatic sac in the guinea pig including tissue and lumen was 506 nl (S.D. 100, n=17) and the volume of the lumen was 169 nl (S.D. 48, n=14).

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PubMed2002

Developing an anatomical model of the human laryngeal cartilages from magnetic resonance imaging.

The purpose of this work was to construct a three-dimensional anatomical framework of the cartilages of the human larynx. The framework included representative surface models of the four laryngeal cartilages and estimated attachment points for the intrinsic laryngeal muscles. High-resolution magnetic resonance imaging (MRI) was used to scan one female and four male human cadaveric larynges. The cartilages were segmented manually from the MRI volume for analysis. Two of these larynges were subsequently dissected and the landmark distances on the cartilages measured for comparison with the MRI measures and previous studies. The MRI measures were 8% smaller than the anatomical measures and 12% smaller than data reported in the literature. A laryngeal coordinate system was defined using the plane of symmetry of the cricoid cartilage. Measures of cricoid cartilage symmetry had less than 3% difference between the two sides for a series of measures. An algorithm for registering larynges that minimized the root-mean-square distance between the surface of a reference cricoid cartilage and the surfaces of nonisotropically scaled candidate cricoid cartilages was evaluated. This study provided an anatomical framework for registering different larynges to the same coordinate space.

Aged↗

PubMed2002

Morphologic phenotyping with MR microscopy: the visible mouse.

A method for rapid morphologic phenotyping is demonstrated by using magnetic resonance microscopy. Whole fixed C57BL/6J mice were imaged at 110-microm isotropic resolution; limited volumes of the intact specimen, at 50-microm isotropic resolution; and isolated organs, at 25-microm isotropic resolution. The three-dimensional imaging technique was applied to uricase knockout mice to demonstrate the method for the evaluation of morphologic phenotype.

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