Relation of nm23 gene expression to CT sign and prognosis in peripheral nonsmall cell lung cancer.
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Adult↗
Biomedical subjects
Shu-hua Ma
Publications and source records attributed to Shu-hua Ma.
[Optimization of hybridization efficiency in cDNA chip technology].
AIM: There are many factors affecting hybridization in cDNA chip: DNA concentration immobilized on glass surface, spotting solution dissolving DNA, the concentration and purity of fluorescence-labeled probe, hybridization solution, hybridization temperature, hybridization time, Cy3- and Cy5-labeled probes, etc. In order to improve hybridization efficiency, tests were designed to optimize these factors. METHODS: Factors are changed one by one. Optimal values are selected by comparing those of different groups. RESULTS AND CONCLUSION: The efficient hybridization condition is as follows: 0.50 microgram.microL-1 DNA fragments resolved in 1 x Micro Spotting Solution (ArrayIt) hybridize with purified fluorescent probe in 0.5% SDS/10 x SSC at 42 degrees C.
Brain Chemistry↗