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PubMed · 12579965

[Optimization of hybridization efficiency in cDNA chip technology].

Shu-hua Ma·Sheng-qi Wang
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Abstract

AIM: There are many factors affecting hybridization in cDNA chip: DNA concentration immobilized on glass surface, spotting solution dissolving DNA, the concentration and purity of fluorescence-labeled probe, hybridization solution, hybridization temperature, hybridization time, Cy3- and Cy5-labeled probes, etc. In order to improve hybridization efficiency, tests were designed to optimize these factors. METHODS: Factors are changed one by one. Optimal values are selected by comparing those of different groups. RESULTS AND CONCLUSION: The efficient hybridization condition is as follows: 0.50 microgram.microL-1 DNA fragments resolved in 1 x Micro Spotting Solution (ArrayIt) hybridize with purified fluorescent probe in 0.5% SDS/10 x SSC at 42 degrees C.

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Brain ChemistryDNA, ComplementaryHumansNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisRNAEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Shu-hua Ma, Sheng-qi Wang. 2002. [Optimization of hybridization efficiency in cDNA chip technology].. https://pubmed.ncbi.nlm.nih.gov/12579965/

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