Biomedical subjects

Susan E Lanzendorf

Publications and source records attributed to Susan E Lanzendorf.

3 recordsLinked to original sources

PubMed2007

A randomized, prospective study comparing laser-assisted hatching and assisted hatching using acidified medium.

OBJECTIVE: To compare the clinical outcomes of patients whose transferred embryos underwent either laser-assisted hatching or hatching with acidified medium. DESIGN: Randomized, prospective, double-blinded study. SETTING: University-based IVF center. PATIENT(S): Clinical outcomes following IVF were compared in 159 patient cycles who randomly had all transferred embryos undergo laser-assisted hatching (Laser; n = 57) or hatching with acidified medium (Acid; n = 54). Patients whose embryos had zonae thickness <13 mum were not hatched (Thin; n = 8) and patients with > or =4 embryos at the 7- to 8-cell stage or higher on Day 3 were transferred on Day 5 (Day 5; n = 40). MAIN OUTCOME MEASURE(S): Implantation rates, clinical pregnancy rates, ongoing pregnancy rates, multiple pregnancy rates. RESULT(S): No significant differences were noted in clinical, ongoing, or multiple pregnancy rates between the four groups. The implantation rate for Day 5 transfers was significantly greater than both the Laser and Acid treatment groups. CONCLUSION: The use of a 1.48-micron infrared diode laser beam provides a safe and rapid method for performing assisted hatching and did not have a negative impact on patient care when compared to outcomes achieved using acidified medium.

Adult↗

PubMed2006

Developmental potential of in vitro- and in vivo-matured human oocytes collected from stimulated and unstimulated ovaries.

The study by Li and coworkers demonstrated that human oocytes collected from the unstimulated ovary have high rates of meiotic spindle and chromosome abnormalities. Until improvements can be made to culture conditions, in vitro maturation protocols for patient treatment should be considered experimental.

Female↗

PubMed2003

Randomized controlled study of human zona pellucida dissection using the zona infrared laser optical system: evaluation of blastomere damage, embryo development, and subsequent hatching.

OBJECTIVE: To assess the effect of laser hatching on human embryo damage and subsequent development using the Zona Infrared Laser Optical System (ZILOS). DESIGN: Randomized controlled study. SETTING: Tertiary care fertility clinic. PATIENT(S): One hundred fourteen donated and discarded frozen human embryos. INTERVENTION(S): Embryos were thawed, cultured with cleavage and morphology evaluated periodically, and randomized into control, partial hatching, or complete hatching groups. The laser hatching procedure was performed by ZILOS. Zona thickness and embryo diameter were recorded. Complete hatching involved the production of a full-thickness defect in the zona and partial hatching, a defect in the outer half of the zona. No laser treatment was administered to the control group. MAIN OUTCOME MEASURE(S): Blastocyst development and completion of hatching process. RESULT(S): No significant difference was noted between the three study groups for their baseline characteristics. There was no significant difference in blastocyst development among the three groups. However, the complete hatching group showed a significant increase in hatching compared to the control group. CONCLUSION(S): Complete laser hatching of human embryos using the ZILOS does not have an adverse effect on subsequent development and increases the rate of completion of hatching.

Blastomeres↗