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Biomedical subjects

T Asao

Publications and source records attributed to T Asao.

7 recordsLinked to original sources

Preparation polyacrylamide-agarose gel electrophoresis of proteins and RNAs by the use of new device.

Quantitatively reproducible results were obtained by using a new device for preparation gel electrophoresis combined with polyacrylamide-agarose composite gel. When an adequate gel-buffer system was selected according to the procedure described in this paper, proteins and RNA's were well separated and recovered. The new device for preparative gel electrophoresis and the method for preparation of polyacrylamide-agarose composite gel are presented together with the elution profiles of the recovered substances.

Blood Proteins

Chordoid sarcoma of the soft tissue of the nape of the neck: a case with a 20 year follow-up.

A huge tumor mass arising from the soft tissue of the nape of the neck, which histologically resembled chordoma is reported. This case had a history of over 30 years after onset at around 37 years of age. With frequent recurrence and re-excision after en bloc radical resection, performed at 47 years of age, this female patient expired at the age of 66, 20 years after the first radical operation. At operation, the mass was found to be firmly attached to the dorsal soft tissue. There was no connection with any bony structure, such as the cervical vertebrae or skull. Chordoma-like histological features were unchanged throughout the next 20 years. This unusual malignant neoplasm appears to correspond to the "chordoid tumor" or "chordoid sarcoma" of soft tissue, a term coined by F.W. Stewart in 1948. Alternatively the name "chondroid chordoma" used by Heffelfinger, Dahlin and others may be employed.

Aged

Sinus histiocytosis with massive lymphadenopathy (Rosai and Dorfman) and significant skin involvement.

Clinicopathological features of this female patient described here, showing unusual and extremely marked sinus histiocytosis of lymph nodes with considerable systemic lymphadenopathy, accompanying fever as well as acceleration of erythrocyte sedimentation rate fairly well coincided with the symptoms of "Sinus Histiocytosis With Massive Lymphadenopathy" (Rosai and Dorfman). The onset of the present case was in 1966 when the patient was 10 years of age, and she has been followed-up for over 10 years. While persisting to display active clinical manifestations, immunological abnormality with thymus involution was demonstrated--deterioration of cell-mediated immunity, but with non-remarkable humoral immunological data, except for hypergammaglobulinemia with elevation of IgG. In 1973 lymphadenopathy as well as skin eruption extended over the extremities and abnormalities of hematological and serological nature began to improve and at the present date the patient is uneventful. This is the first recorded case of "Sinus Histiocytosis With Massive Lymphadenopathy" (Rosai and Dorfman) in Japan. Considerable skin involvement in the upper and lower extremities was a significant feature and unique in this case.

Adult