Quantitative analysis of proteins by the use of SDS-polyacrylamide-gel electrophoresis.

[A fast and simple micromethod for estimating proteins].

The assay is based on the property of proteins to form a complex with bromophenol blue. In alkaline solution this complex shows an intensive blue colour which is well measurable at 598 nm. The method is especially useful for small volumes (5-50 microliter) with a content of 5-30 micrograms of protein. By modification the test can be also used for the measurement of higher amounts of protein.