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T Höhler

Publications and source records attributed to T Höhler.

53 records · Page 3Linked to original sources
PubMed1996

TAP-polymorphisms in juvenile onset psoriasis and psoriatic arthritis.

Juvenile onset psoriasis is strongly associated with the HLA-class I genes Cw6 and B57 whereas patients with psoriatic arthritis show an increased frequency of HLA-B27. It is unclear whether additional major histocompatibility genes also increase disease susceptibility. The TAP genes (transporter associated with antigen processing) encode two membrane-spanning proteins that translocate antigenic peptides from the cytoplasm into the endoplasmic reticulum. Comparison of 60 patients with juvenile onset psoriasis, 63 psoriatic arthritis patients, and 101 caucasoid controls revealed an increase of the TAP1*0101 allele in the psoriasis group, that could not be explained by linkage to other investigated HLA genes. There were no differences for TAP2 alleles.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1996

Antigen-processing polymorphisms in chronic hepatitis C infection.

BACKGROUND: Cytotoxic T cell (CTL) responses may influence the course of a hepatitis C infection. CTL recognize viral peptides on HLA class I molecules. Antigenic peptides for class I presentation are generated by proteasomes and transported into the endoplasmic reticulum by the TAP proteins. It is unknown whether polymorphisms of the antigen-processing genes might influence the development of chronic hepatitis C. METHODS: 75 patients with chronic hepatitis C and 99 unrelated controls were typed for TAP and LMP2 polymorphisms. Typing was done by allele-specific PCR techniques and PCR with subsequent single-stranded confirmation polymorphism (SSCP) analysis. RESULTS: Apart from minor increases of the TAP2*0101 allele and LMP2 heterozygotes in the chronic hepatitis group there were no statistically significant differences between the two groups. CONCLUSIONS: These findings suggest, that disease association for chronic hepatitis C does not extend beyond the HLA-DQ locus. Although TAP and LMP play an important role in the processing of viral antigens, their limited polymorphisms do not show any association with the development of chronic hepatitis C.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1995

Complement component C3: molecular basis of the C3*S025 variant and evidence for molecular heterogeneity of other variants.

Complement component 3 (C3) is the central molecule of the complement system. It displays a number of polymorphic variants with, as yet, unclear functional consequences. We have investigated a number of rare C3 variants by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) analysis and could identify the molecular basis of a C3*S025 variant. The decreased electrophoretic mobility of this protein is caused by the exchange of a neutral serine residue to an arginine residue (positively charged). This exchange is unlikely to have functional consequences as it maps to the C-terminus of the alpha-chain. C3 variants appear to have originated from various independent mutations as we could not detect this mutation in different allotypes.

Base Sequence↗
PubMed1995

Cholinesterase variants: rapid characterisation by PCR/SSCP and evidence for molecular homogeneity.

We have applied the technique of PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism) to characterise the molecular basis of cholinesterase deficiency and variants in a Jordanian family. PCR-SSCP proved to be a quick and sensitive method of screening cholinesterase variants in a clinical setting. An AG insertion at position 351 was found to cause a silent allele, for which the parents were heterozygous and three children homozygous. In addition, the father and two sons were heterozygous for an A to G transition at position 209, known to cause the dibucaine resistant variant. No linkage to the K variant was found, which has been reported previously in white populations. These findings suggest considerable homogeneity in the molecular basis of CHE variants between different ethnic groups.

Adult↗
PubMed1995

Pulmonary aspergilloma in a patient with AIDS.

Aspergillus infections are rare in the course of AIDS. They mostly occur as invasive destructive disease in patients with severe CD4 cell depletion. An unusual case of a homosexual AIDS patient who developed a pulmonary aspergilloma is presented.

AIDS-Related Opportunistic Infections↗
PubMed1994

Indomethacin treatment in amphotericin B induced nephrogenic diabetes insipidus.

Nephrogenic diabetes insipidus (NDI) is a serious side effect of various drugs. Elevated renal prostaglandin E2 levels have been found in patients with lithium-induced NDI and have been implicated in the pathogenesis. We report the case of a patient who developed NDI following treatment with amphotericin B. Prostaglandin levels were elevated. Indomethacin had an antidiuretic effect and normalized prostaglandin levels.

Amphotericin B↗
PubMed1994

Drug-induced hepatitis: a rare complication of oral anticoagulants.

Hepatotoxicity is a rare complication of coumarin anticoagulants. We present the case of a 56-year-old woman who developed a viral-hepatitis-like picture 8 months after mitral valve replacement and oral anticoagulation. Phenprocoumon-induced hepatitis was diagnosed after positive reexposure and improvement following withdrawal of the drug. There appeared to be cross-reactivity to warfarin since this drug led to a similar increase in alkaline phosphatase and gamma-glutamyl transferase after a few days of administration. Liver biopsy showed an acute viral-hepatitis-like picture. Anticoagulation was changed to a subcutaneous low molecular weight heparin and low-dose aspirin. Because of the widespread use of coumarin anticoagulants, physicians should be aware of the hepatotoxic potential of these drugs, which most frequently mimics the clinical presentation of viral hepatitis.

Administration, Oral↗
PubMed1993

[Topical therapy of onychomycoses with 8% ciclopirox laquer. An open, non-comparative study].

AIM: To investigate the efficacy and tolerability of 8% Ciclopirox-containing nail varnish used for topical treatment of onychomycoses in an open single-center study. METHOD: Forty-seven patients with onychomycoses affecting a finger and/or toe nail were treated with nail varnish containing Ciclopirox once daily for 6 months. The results of treatment were monitored at regular intervals during treatment, and for 6 months after termination of therapy. RESULTS: By the end of treatment, culture for fungi was negative in all the patients investigated; clinical healing was observed in 26 patients (56.5%) and clinical improvement in 13 (28%). In seven patients, however, the clinical findings had hardly changed. By the end of the 6-month follow-up phase, the healing rate (mycological and clinical) was 59.5% (28/47 patients), improvement (mycological healing, clinical improvement) was 12.8% (6/47 patients) and failures (neither mycological nor clinical healing) was 27.7% (13/47 patients). Mild distal subungual onychomycoses and superficial white onychomycoses are the main indications for topical antimycotic treatment. In contrast, extensive distal subungual nail mycoses, proximal subungual and total dystrophic onychomycoses in which the matrix of the nail already appears to be involved, represents the limitations of topical treatment. In addition to the high level of efficacy, which depends upon the extent and form of nail involvement, the substance was very well tolerated; in none of the patients were any side effects observed.

Administration, Topical↗
PubMed1993

Studies on the pharmacokinetics and metabolism of prednicarbate after cutaneous and oral administration.

Prednicarbate (PC) is a nonhalogenated derivative of prednisolone which is used for the local treatment of corticoid-sensitive skin diseases. In this study, the pharmacokinetics and the metabolism of PC in humans are investigated after cutaneous ointment application (75 mg PC) and after systemic oral administration (40 mg PC) in 8 healthy volunteers. In addition, the possible suppression of endogenous cortisol secretion by both application forms was monitored. After oral administration no intact PC, but significant levels of the first metabolite prednisolone-17-ethylcarbonate (PRED-17-EC) were determined. PRED-17-EC was further metabolized with a half life of 1.6 h to prednisolone. After percutaneous administration neither PC nor other known metabolites could be detected systemically. The low systemic bioavailability after dermal application was also reflected in an unchanged cortisol secretion pattern. According to animal studies our metabolic studies in humans suggest that the prednisolone-17-ester PRED-17-EC, which has a receptor binding affinity comparable to that of dexamethasone is the pharmacologically active compound. As PRED-17-EC subsequently undergoes an inactivation step to the low active prednisolone this may be the reason for the dissociation of good local efficacy and low systemic side effects.

Administration, Oral↗
PubMed1990

[The psychosocial status of patients with endogenous eczema. A study using cluster analysis for the correlation of psychological factors with somatic findings].

The present study was performed to investigate whether patients with atopic dermatitis differ as a group from controls on psychological measures of mood and personality or whether psychologically deviant and normal patient subgroups can be distinguished. Furthermore, we were interested in what clinical characteristics might co-vary with psychological disability in patients with atopic dermatitis. In all, 93 patients filled in a standardized mood scale (Hamburg-Erlanger-Stimmungsbarometer) and a personality scale (Kurztest zur Erfassung der Persönlichkeitsstruktur). Compared with matched controls, patients described themselves as being more anxious, more aroused, more depressed and less energetic, and they reached higher neuroticism scores. A cluster analysis identified four patient subgroups. Only one of the subgroups (n = 17) was psychologically disabled according to the questionnaire scores. In contrast to a psychologically stabile patient group, the psychologically disabled patients showed an earlier age of onset of dermatitis, but less intense itching and scratching. They reported more somatic complaints and a higher level of familial stress, were more dissatisfied with their life situation and work, had fewer friends and experienced more losses of significant others. Furthermore, they more frequently rated their disorder as being determined by psychological factors and were more intelligent. Thus, the questionnaires identified a subgroup of patients who may need psychotherapeutic interventions.

Adolescent↗
PubMed1981

[On the local efficacy of ciclopiroxolamine in onychomycoses (author's transl)].

An open, multicenter clinical trial of 6-cyclohexyl-1-hydroxy-4-methyl-2(1H)-pyridone, 2-aminoethanol salt (ciclopiroxolamine, Cic, Hoe 296, Batrafen) was performed in 150 patients with 246 nails afflicted by mycotic infection. Dermatophytes were observed in 96% of the initial cultures. A combination of 1% Cic solution and cream was applied, and a very strict nail toilet was performed. The mean duration of study was 12.7 +/- 5.6 weeks, followed by a 6 week follow-up period on the average. The share of pathologically changed nails of 35 +/- 16% was reduced to 8 +/- 10% of nail plate. In a total of 96% of patients, some of whom had only been treated for a brief period, a measurable improvement was observed, and 57% of the patients were free from signs of infection. Signs of intolerance or definite relapses were not observed.

Adolescent↗
PubMedDate not supplied

LMP polymorphisms do not influence disease expression in psoriatic arthritis.

OBJECTIVES: To investigate the potential role of the HLA-linked LMP2 (low molecular weight protein) and LMP7 gene polymorphisms in conjunction with HLA class I and class II genes on the disease pattern in individuals with psoriatic arthritis (PsA). METHODS: Sixty-three patients with PsA and 99 unrelated controls were typed for HLA-class I and II antigens. LMP2 and LMP7 polymorphisms were determined by PCR and subsequent single stranded conformation polymorphism (SSCP) analysis or restriction enzyme digestion. RESULTS: PsA was associated with B27 (p < 0.0004), B57 (p < 0.002) and Cw2 (p < 0.008). Spondylarthritis was strongly associated with HLA-B27 (p < 0.000001), Cw2 (p < 0.0003) and DR4 (p < 0.008). For the polyarthritic pattern the only association was with B57 (p < 0.007). There was no association between the LMP2 or LMP7 genotypes and any particular disease pattern. CONCLUSIONS: These findings do not support an involvement of the HLA linked LMP2 and LMP7 gene polymorphisms in disease expression in psoriatic arthritis in addition to the known HLA class I and II associations.

Adult↗