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Biomedical subjects

T Hanada

Publications and source records attributed to T Hanada.

At least 181 records · Page 10Linked to original sources
PubMed1983

Sarcomatous Wilms tumor associated with consumption coagulopathy.

This is a case report of a sarcomatous Wilms tumor complicating disseminated intravascular coagulation with gastrointestinal bleeding, ascites, pleural effusion, and hepatitis after the second course of actinomycin D. A sarcomatous Wilms tumor is believed to have an unfavorable prognosis even if multimodal therapy is given. Arteriovenous fistulae which we created for purposes of chemotherapy and hyperalimentation by microvascular-sleeve anastomosis functioned well for three years in spite of repeated punctures.

Arteriovenous Shunt, Surgical↗
PubMed1983

The effect of peripheral blood adherent cells from patients with aplastic anemia on in vitro hematopoiesis.

To investigate the pathogenetic significance of peripheral blood adherent cells in aplastic anemia, the inhibitory effects of adherent cells obtained from the peripheral blood of 10 patients with aplastic anemia on the growth of CFUE and CFUC were compared with those of normal controls. The inhibitory effects of adherent cells were studied by means of co-culture with normal allogeneic bone marrow cells. The suppression of these inhibitory effects was also examined by adding indomethacin to the co-culture system. In addition, PGE2 concentrations in the adherent cell-conditioned media were assayed. The inhibition of the colony growth by peripheral blood adherent cells from the aplastic anemia patients was not significantly different from controls. Both the suppression of the inhibitory effects by indomethacin and the level of PGE2 production showed no significant difference between the patients and controls.

Adult↗
PubMed1982

Inhibitory effects of T cells on in vitro granulopoiesis, erythropoiesis, and immunoglobulin production in patients with aplastic anaemia.

Coculture with mononuclear cells from aplastic anaemia (AA) patients and normal bone marrow cells produced different responses among 10 patients studied. In 4 cases, CFU-E growth was inhibited while in another 2 cases CFU-C growth was inhibited. To determine the suppressive effects of mononuclear cells from 6 AA patients, mononuclear cells were separated into T cells, B cells, and monocytes. It was found that T cells from 6 AA patients exhibited suppressive effects on normal CFU-C or CFU-E growth, whereas T cells from normal subjects enhanced the colony formation of CFU-C and CFU-E. On the other hand, monocytes fom 6 AA patients and normal subjects strongly suppressed normal CFU-C and CFU-E growth, although no significant difference was observed in both groups. The immune system in 10 AA patients was evaluated, 3 AA patients (Cases No. 1, 6, 8) exhibited an increase in Tr cells, impairment of PHA responsiveness, and low level of immunoglobulin. T cells from these patients showed a significant suppressive effect on PWM-induced differentiation of allogenic normal B cells. Thus, T cells from 3 out of 10 AA patients exhibited suppressor activities on both haematopoietic cell differentiation and B cell differentiation into plasma cells in vitro.

Adolescent↗
PubMed1982

Dual effect of monocyte-conditioned medium on in vitro hematopoiesis.

To investigate the effects of the conditioned medium on regulation of hematopoietic stem cells, serial volumes of monocyte-conditioned medium were added to the culture systems of CFUE and CFUC. Small volumes of the conditioned medium possessed stimulatory activity and large volumes inhibitory activity for both CFUE and CFUC. These findings suggest that the monocyte-conditioned medium regulates both CFUE and CFUC dualistically in accordance with the volume of the medium added to the culture system. The suppression of the growth of CFUE and CFUC by monocyte-conditioned medium was completely protected when the medium made in the presence of indomethacin was added. Monocyte mediated suppression was partially recovered when both monocytes and indomethacin were added. E series prostaglandins appear to be inhibitory mediators of monocyte and monocyte-conditioned medium mediated suppression, but some other mechanism seems to be involved in the monocyte mediated suppression.

Colony-Forming Units Assay↗
PubMed1982

Severe aplastic anaemia treated with anti-lymphocyte globulin. The relationship between clinical course and erythroid colony suppression by T cells.

A 6-year-old girl with severe aplastic anaemia improved promptly after treatment with anti-human lymphocyte globulin (ALG). Blood T lymphocytes were proved to have a suppressive effect on erythroid colony formation. ALG was administered intravenously at a dose of 15 mg/kg/d for 5 d. By the 14th d she showed a prompt increase in the reticulocyte count. Within the next 2 weeks slight increase of the platelet count was observed while the neutrophil count remained unchanged. The suppressive effect of T cells on erythroid colony formation weakened when the reticulocyte count exceeded 100 X 10(9)/l. About 4 months after administration of ALG, a decreased reticulocyte count was observed along with frequent nasal bleeding. Again the T cells exhibited a suppressive effect on erythroid colony formation. The results of serial co-culture studies revealed a close correlation between the T-cell suppressive effect on erythroid colony formation and reticulocyte response. The findings suggest an immune-mediated mechanism for the haematopoietic disorder in this patient.

Anemia, Aplastic↗